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Histones Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6242234

MBS6242234 is an antibody targeting Histones. Also known as Histones, pan (APC). Reactive in Bovine, Hamster, Human, Opossum, Rat, Xenopus. Host: Mouse. More details

$1435 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histones, Monoclonal Antibody
Full Product Name
Histones, pan (APC)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q2283
Host
Mouse
Species Reactivity
Bovine, Hamster, Human, Opossum, Rat, Xenopus
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography
Specificity
Recognizes human histones H1, H2A, H2B, H3 and H4 with comparable sensitivity. Binds specifically to an antigen determinant, which is present on all five histone proteins. Species Crossreactivity: bovine, rat, Xenopus, opossum and hamster.
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Immunogen
Purified nuclear fractions of HeLa cells
Conjugate
APC

Product Categories/Family for anti-Histones antibody

Antibodies; Histones
Applications Tested/Suitable for anti-Histones antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)

Western Blot (WB) of anti-Histones antibody

Western Blot analysis in Jurkat lysate (Lane 1) & mouse brain lysate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with H3505 (1:500). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Arrow indicates protein Histone (Pan) (~20kD).
Mouse Anti-Histones Monoclonal Antibody – Cat# MBS6242234

Immunofluorescence (IF) of anti-Histones antibody

Immunofluorescent staining of NIH/3T3 cells using MBS600046 and goat anti-mouse IgG conjugated to Alexa Fluor 350.(blue). Actin filaments have been labeled with Rhodamine Phalloidin (red).
Mouse Anti-Histones Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6242234

Product References and Citations for anti-Histones antibody

1. Kutney, S., et al. (2004). J. Biol. Chem. 279(29): 30850-30855.

Other Notes

Small volumes of anti-Histones antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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