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Lysine Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6242398

MBS6242398 is an antibody targeting Lysine. Also known as Lysine, Acetylated (Acetyl-Lysine) (APC). Reactive in Human. Host: Mouse. More details

$1320 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Lysine, Monoclonal Antibody
Full Product Name
Lysine, Acetylated (Acetyl-Lysine) (APC)
Clonality
Monoclonal
Isotype
IgG
Clone Number
4H349
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes acetylated lysine-containing proteins including histones. Of all the possible H3 and H4 acetylation sites, this antibody exhibits highest affinity for histone H4 acetylated on lysine 8 and histone H3 acetylated on lysine 14. Species Crossreactivity: human. Species Sequence Homology: rat and mouse
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Immunogen
Mixture of chemically acetylated antigens
Conjugate
APC

Product Categories/Family for anti-Lysine antibody

Applications Tested/Suitable for anti-Lysine antibody
Immunoprecipitation (IP), Western Blot (WB)

Testing Data of anti-Lysine antibody

Acid-extracted proteins from untreated (lane 1) or sodium butyrate treated (lane 2) HeLa cells probed with MBS6013338 (4ug/ml).
Mouse Anti-Lysine Monoclonal Antibody – Cat# MBS6242398

Testing Data of anti-Lysine antibody

Representative lot data. Untreated and sodium butyrated treated HeLa lysate was resolved by electrophoresis, transferred to PVDF membrane and probed withMBS6013338. Proteins were visualized using a IgG (HRP)goat anti-mouse secondary antibody and a chemiluminescence detection system.
Mouse Anti-Lysine Monoclonal Antibody, Testing Data – Cat# MBS6242398

Testing Data of anti-Lysine antibody

Proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS6013338 (4ug/ml). Panel A: RIPA lysates of untreated (Lane 1) and Trichostatin A treated (5um, 24 hours) Cos-1 cells (Lane 2). Panel B: In vitro acetylation of GST-p53 in the absence (Lane 1) or presence of recombinant p300 (Lane 2).
Mouse Anti-Lysine Monoclonal Antibody, Testing Data – Cat# MBS6242398

Product References and Citations for anti-Lysine antibody

1. Pennisi, E., Science (1997) 275: 155. 2. Imhof, A., et al., (1997) Current Biology 7: 689. 3. Munshi, N., et al., (1998) Mol. Cell. 2: 457. 4. Liu, L., et al., (1999) Mol. Cell. Biol. 19: 1202. 5. Cesena, Teresa, et al. (2007) J. Biol. Chem. 282: 956-967.

Other Notes

Small volumes of anti-Lysine antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Lysine. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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