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mTOR Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6242459

MBS6242459 is an antibody targeting mTOR. Also known as mTOR (Mammalian target of rapamycin, mechanistic target of rapamycin (serine/threonine kinase), FK506-binding Protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated… More details

$1250 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
mTOR, Monoclonal Antibody
Full Product Name
mTOR (Mammalian target of rapamycin, mechanistic target of rapamycin (serine/threonine kinase), FK506-binding Protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, Rapamycin Ta
Product Gene Name
anti-mTOR antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
9H256
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human mTOR, ~289kD.
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Molecular Weight
288,892 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (11166588..11322608, complement). Location: 1p36.2
OMIM
609022
3D Structure
ModBase 3D Structure for Q6R327
Immunogen
Full-length human mTOR (NP_004949.1).
Conjugate
APC

Product Categories/Family for anti-mTOR antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-mTOR antibody
Immunocytochemistry (ICC), Western Blot (WB)

Immunofluorescence (IF) of anti-mTOR antibody

Confocal Immunofluorescent Immunocytochemistry on HeLa cells. mTOR is in red using M4696-10D.
Mouse Anti-mTOR Monoclonal Antibody – Cat# MBS6242459

Immunocytochemistry (ICC) of anti-mTOR antibody

Confocal Immunofluorescent Immunocytochemistry Representative lot data using M4696-10D HeLa cells (top) and A431 cells (bottom) were triple stained with M4696-10D (Red, Cy3) (1:500), Actin (Phalloidin, Green), and DAPI (nuclei, blue). The figures on the left are with the green 488 filter off.
Mouse Anti-mTOR Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS6242459

SDS-Page of anti-mTOR antibody

Representative lot data using M4696-10D HEK293 cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane, and probed with M4696-10D (1:2000). Proteins were visualized using an IgG (HRP) donkey anti-mouse secondary antibody and a chemiluminescence detection system. Arrow indicates mTOR (289kD).
Mouse Anti-mTOR Monoclonal Antibody, SDS-Page – Cat# MBS6242459

Product References and Citations for anti-mTOR antibody

1. Lawrence, JC., et al. (2002). JBC. Vol. 277, No. 20: 17657-17662. 2. Sarbassov, DD., et al., (2005). Science, Vol. 307, No. 5712: 1098-1101. 3. Sabatini, D. (2006). Nature Reviews Cancer, Vol. 6, 729-734.

Other Notes

Small volumes of anti-mTOR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for mTOR. It may not necessarily be applicable to this product.

NCBI GI #
4826730
NCBI GeneID
2475
NCBI Accession #
NP_004949.1
UniProt Primary Accession #
Q6R327
UniProt Secondary Accession #
Q4LE76; Q5TER1; Q6LE87; Q96QG3; Q9Y4I3
UniProt Related Accession #
P42345
GenBank Nucleotide #
NM_004958.3
NCBI Official Full Name
serine/threonine-protein kinase mTOR
NCBI Official Synonym Full Names
mechanistic target of rapamycin (serine/threonine kinase)
NCBI Official Symbol
MTOR
NCBI Official Synonym Symbols
FRAP; FRAP1; FRAP2; RAFT1; RAPT1
UniProt Protein Name
Rapamycin-insensitive companion of mTOR
UniProt Synonym Protein Names
AVO3 homolog; hAVO3
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
RICTOR
UniProt Synonym Gene Names
hAVO3
UniProt Entry Name
RICTR_HUMAN
NCBI Protein Information
serine/threonine-protein kinase mTOR; rapamycin target protein 1; mammalian target of rapamycin; rapamycin and FKBP12 target 1; FKBP-rapamycin associated protein; rapamycin associated protein FRAP2; FKBP12-rapamycin complex-associated protein 1; FK506 bin

NCBI Summary for mTOR

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
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UniProt Comments for mTOR

RICTOR: a regulatory binding partner of the kinase mTOR. Part of a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Rictor interacts with Cullin1-Rbx1 to form an E3 ubiquitin ligase complex, and promotes ubiquitination and degradation of SGK1. Shares homology with pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae.

Protein type: Cell cycle regulation; Ubiquitin conjugating system; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: TORC2 complex; cytosol

Molecular Function: protein binding; ribosome binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; TOR signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; regulation of protein kinase B signaling cascade; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; embryonic development; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; actin cytoskeleton reorganization; innate immune response; positive regulation of endothelial cell proliferation; vascular endothelial growth factor receptor signaling pathway; positive regulation of TOR signaling pathway
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Research Articles on mTOR

1. The TNF-alpha-mediated in vitro RPE cell migration mainly requires Akt/mTORC1, but not mTORC2 signaling. The results of this study may lead to indentify novel signaling targets against PVR.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-mTOR antibody

 Reference Product  PubMed Publications
 EIF4EBP1 antibody  >444 publications with mTOR and EIF4EBP1
 AKT1 antibody  >342 publications with mTOR and AKT1
 RICTOR antibody  >243 publications with mTOR and RICTOR
 RHEB antibody  >233 publications with mTOR and RHEB
 STAT3 antibody  >155 publications with mTOR and STAT3
 IRS1 antibody  >117 publications with mTOR and IRS1
 RPTOR antibody  >109 publications with mTOR and RPTOR
 RPS6KA1 antibody  >74 publications with mTOR and RPS6KA1
 AKT1S1 antibody  >21 publications with mTOR and AKT1S1
 TERT antibody  >19 publications with mTOR and TERT

Pathways associated with anti-mTOR antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram

Diseases associated with anti-mTOR antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4311 publications with mTOR and Neoplasms
 Nervous System Diseases Antibodies  >776 publications with mTOR and Nervous System Diseases
 Adenocarcinoma Antibodies  >772 publications with mTOR and Adenocarcinoma
 Kidney Diseases Antibodies  >635 publications with mTOR and Kidney Diseases
 Breast Neoplasms Antibodies  >520 publications with mTOR and Breast Neoplasms
 Drug Toxicity Antibodies  >435 publications with mTOR and Drug Toxicity
 Cardiovascular Diseases Antibodies  >424 publications with mTOR and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >379 publications with mTOR and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >363 publications with mTOR and Lung Neoplasms
 Hypertrophy Antibodies  >336 publications with mTOR and Hypertrophy

Organs/Tissues associated with anti-mTOR antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1270 publications with mTOR and Blood
 Muscle Antibodies  >1066 publications with mTOR and Muscle
 Kidney Antibodies  >1032 publications with mTOR and Kidney
 Vascular Antibodies  >930 publications with mTOR and Vascular
 Brain Antibodies  >893 publications with mTOR and Brain
 Liver Antibodies  >692 publications with mTOR and Liver
 Lung Antibodies  >601 publications with mTOR and Lung
 Heart Antibodies  >430 publications with mTOR and Heart
 Bone Antibodies  >321 publications with mTOR and Bone
 Prostate Antibodies  >309 publications with mTOR and Prostate
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