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WTX Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS624482

MBS624482 is an antibody targeting WTX. Also known as WTX, ID (Protein FAM123B, Wilms Tumor Gene On The X Chromosome Protein, FAM123B). Reactive in Human, Mouse. Host: Rabbit. WTX is involved in kidney development. More details

$895 / 0.2 mL +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
WTX, Polyclonal Antibody
Full Product Name
WTX, ID (Protein FAM123B, Wilms Tumor Gene On The X Chromosome Protein, FAM123B)
Product Synonym Names
Anti -WTX, ID (Protein FAM123B, Wilms Tumor Gene On The X Chromosome Protein, FAM123B)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human WTX. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the Center region of human WTX (KLH).

Product Categories/Family for anti-WTX antibody

Antibodies; Abs to Cancer Markers
Applications Tested/Suitable for anti-WTX antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-WTX antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-WTX antibody

Western blot analysis of WTX antibody (Center) in mouse kidney tissue lysates (35ug/lane). WTX (arrow) was detected using the purified Pab.
Rabbit Anti-WTX Polyclonal Antibody – Cat# MBS624482

Immunohistochemistry (IHC) of anti-WTX antibody

WTX Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WTX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-WTX Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS624482

Flow Cytometry (FC/FACS) of anti-WTX antibody

WTX Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-WTX Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS624482

Other Notes

Small volumes of anti-WTX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WTX. It may not necessarily be applicable to this product.

NCBI GI #
121556878
NCBI Official Full Name
WTX
Protein Family
Weak toxin

NCBI Summary for WTX

The protein encoded by this gene upregulates trancriptional activation by the Wilms tumor protein and interacts with many other proteins, including CTNNB1, APC, AXIN1, and AXIN2. Defects in this gene are a cause of osteopathia striata with cranial sclerosis (OSCS). [provided by RefSeq, May 2010]

UniProt Comments for WTX

WTX: a peripheral membrane protein on the cytoplasmic side that shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Enhances trancription activation by WT1. Promotes CTNNB1 ubiquitination and degradation. Antagonizes Wnt and CTNNB1 signaling. Inactivated in approximately one-third of Wilms tumors. Detected in fetal and adult kidney, brain and spleen. Involved in kidney development. Interacts with CTNNB1, AXIN1, KEAP1, APC and BTRC. Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes containing BTRC and/or FBXW11. Identified in a complex containing CTNNB1, APC, AXIN1 and AXIN2. Two alternatively spliced human isoforms have been reported.

Chromosomal Location of Human Ortholog: Xq11.2

Cellular Component: plasma membrane; nucleus; cytosol

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; beta-catenin binding

Biological Process: Wnt receptor signaling pathway

Disease: Osteopathia Striata With Cranial Sclerosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-WTX antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with WTX and FLNA

Pathways associated with anti-WTX antibody

 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram

Organs/Tissues associated with anti-WTX antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with WTX and Bone
 Connective Tissue Antibodies  >4 publications with WTX and Connective Tissue
 Heart Antibodies  >3 publications with WTX and Heart
 Muscle Antibodies  >3 publications with WTX and Muscle
 Brain Antibodies  >3 publications with WTX and Brain
 Vascular Antibodies  >2 publications with WTX and Vascular
 Embryonic Tissue Antibodies  >2 publications with WTX and Embryonic Tissue
 Ear Antibodies  >1 publications with WTX and Ear
 Kidney Antibodies  >1 publications with WTX and Kidney
 Umbilical Cord Antibodies  >1 publications with WTX and Umbilical Cord
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