Mouse Anti-H1-4 Monoclonal Antibody – Cat# MBS6245224

Jurkat cell lysate was probed with MBS602894 at 0.5ug/ml. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Arrow indicates Histone H1 + core proteins (~14-33kD).

Histones (H1-4) Antibody – Mouse Monoclonal

Name: H1-4 Antibody; Monoclonal; Mouse; Bovine/Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6245224
Price: 1275 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6245224

MBS6245224 is an antibody targeting H1-4. Also known as Histones (MaxLight 405). Reactive in Bovine, Hamster, Human. Host: Mouse. More details

$1275 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Histones (H1-4), Monoclonal Antibody

Full Product Name

Histones (MaxLight 405)

Product Gene Name

anti-H1-4 antibody

Clonality

Monoclonal

Isotype

IgG2a,k

Clone Number

2Q2205

Host

Mouse

Species Reactivity

Bovine, Hamster, Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

Purity/Purification

Purified by Protein G Affinity Chromatography

Specificity

Recognizes human Histones, H1 and core proteins H2a, H2b, H3, H4. Species Crossreactivity: bovine and hamster

Preparation and Storage

Store at 4 degree C. Do Not Freeze.

Molecular Weight

1433

OMIM

142220

3D Structure

ModBase 3D Structure for P10412

Immunogen

Histones purified from calf thymus. MW: 15-33kD.

Conjugate

MaxLight405

Product Categories/Family for anti-H1-4 antibody

Antibodies; Histones

Applications Tested/Suitable for anti-H1-4 antibody

FLISA, Western Blot (WB)

Product References and Citations for anti-H1-4 antibody

1. Federation Proceedings (1984). 43:2063. 2. Mizoguchi, E., et al., Antineutrophil Cytoplamic Antibodies in T-Cell Receptor •-Deficient Mice with Chronic Colitis. Gastroenterology (1997) 113:1828-1835. 3. Feng, H., et al., CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans. Nature Cell Biology (1999) 1:486- 492. 4. Brimmell, M., et al., High level expression of differentially localized BAG-1 isoforms in some oestrogen receptor-positive human breast cancers. British Journal of Cancer (1999) 81:1042-1051.

Other Notes

Small volumes of anti-H1-4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for H1-4. It may not necessarily be applicable to this product.

NCBI GI #

20544164

NCBI GeneID

3008

NCBI Accession #

NM_005321.2

UniProt Primary Accession #

P10412

UniProt Related Accession #

P10412

GenBank Nucleotide #

NM_005321.2

NCBI Official Full Name

Homo sapiens H1.4 linker histone, cluster member (H1-4), mRNA

NCBI Official Synonym Full Names

H1.4 linker histone, cluster member

NCBI Official Symbol

H1-4

NCBI Official Synonym Symbols

H1E; H1.4; H1F4; RMNS; H1s-4; HIST1H1E; dJ221C16.5

UniProt Protein Name

Histone H1.4

UniProt Synonym Protein Names

Histone H1b

UniProt Gene Name

HIST1H1E

UniProt Synonym Gene Names

H1F4

UniProt Entry Name

H14_HUMAN

NCBI Protein Information

histone H1.4

NCBI Summary for H1-4

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Research Articles on H1-4

1. Crystallographic studies of an histone H1.4K26me3:lysine demethylase 4A (KDM4A) complex iidicate a conserved binding geometry to that of H3K9me3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.