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SMARCA4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6245727

MBS6245727 is an antibody targeting SMARCA4. Also known as SMARCA4 (Transcription Activator BRG1, ATP-dependent Helicase SMARCA4, BRG1-associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1,… More details

$1335 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SMARCA4, Monoclonal Antibody
Full Product Name
SMARCA4 (Transcription Activator BRG1, ATP-dependent Helicase SMARCA4, BRG1-associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-beta, SWI/SNF-related Matrix-associated Actin-dependent
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
13A219
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human SMARCA4. Species Crossreactivity: rat.
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Molecular Weight
184
OMIM
603254
3D Structure
ModBase 3D Structure for P51532
Immunogen
Recombinant protein corresponding to human SMARCA4 fused to GST-tag.
Conjugate
MaxLight490

Product Categories/Family for anti-SMARCA4 antibody

Applications Tested/Suitable for anti-SMARCA4 antibody
Immunocytochemistry (ICC), Western Blot (WB)

Immunocytochemistry (ICC) of anti-SMARCA4 antibody

Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of HeLa and A431 cells using SMARCA4 (Red). Actin filaments have been labeled with AlexaFluor® 488 dye Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains nucleus.
Mouse Anti-SMARCA4 Monoclonal Antibody – Cat# MBS6245727

Western Blot (WB) of anti-SMARCA4 antibody

Western Blotting Analysis: Representative lot data. HeLa nuclear extract was probed with SMARCA4, clone 20C3.2 (0.5ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SMARCA4 (~184kD).
Mouse Anti-SMARCA4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6245727

Product References and Citations for anti-SMARCA4 antibody

1. Schneppenheim, R., et al. (2010). Am J Hum Genet. 86(2):279-284. 2. Fujimoto, M., et al. (2010). Mol Biol Cell. 21(1):106-116. 3. Zager, R.A., and Johnson, A.C. (2009). Am J Physiol Renal Physiol. 296(5):F1032-1041. 4. Kenneth, N.S., et al. (2009). J Biol Chem. 294(7):4123-4131. 5. Voss, T.C., et al. (2009). J Cell Sci. 122(Pt 3):345-356. 6. Osipovich, O., et al. (2007). Nat Immunol. 8(8):809-816. 7. Dammer, E.B., Leon, A., and Sewer, M.B. (2007). Mol Endocrinol. 21(2):415-438. 8. Ramirez-Carrozzi, V.R., et al. (2006). Genes Dev. 20(3):282-296. 9. Sif, S, et al. (1998). Genes Dev. 12(18):2842- 2851. 10. Schnitzler, G., Sif, S., and Kingston, R.E. (1998). Cell. 94(1):17-27.

Other Notes

Small volumes of anti-SMARCA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SMARCA4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription activator BRG1 isoform B
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
NCBI Official Symbol
SMARCA4
NCBI Official Synonym Symbols
BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; SNF2-beta
UniProt Protein Name
Transcription activator BRG1
UniProt Synonym Protein Names
ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
UniProt Gene Name
SMARCA4
UniProt Synonym Gene Names
BAF190A; BRG1; SNF2B; SNF2L4; BAF190A
UniProt Entry Name
SMCA4_HUMAN
NCBI Protein Information
transcription activator BRG1

NCBI Summary for SMARCA4

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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