Rabbit Anti-CFL2 Polyclonal Antibody – Cat# MBS624852

Western Blot of L6 lysate was incubated with (lane 2) or without (lane 1) LIM Kinase at 30ºC for 30 minutes. Aliquots were probed with MBS624852.

Cofilin 2 (CFL2) Antibody – Rabbit Polyclonal

Name: CFL2 Antibody; Polyclonal; Rabbit; Chicken/Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS624852
Price: 1155 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS624852

Cofilin is a small actin-binding protein that plays a pivotal role in cytokinesis, endocytosis, embryonic development, stress response and tissue regeneration. More details

$1155 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cofilin 2 (CFL2), Polyclonal Antibody

Full Product Name

Cofilin 2, phosphorylated (Ser3)

Product Gene Name

anti-CFL2 antibody

Product Synonym Names

Anti -Cofilin 2, phosphorylated (Ser3)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Chicken, Human, Mouse

Form/Format

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human phospho-Cofilin 2, Mr 21kD.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

18,737 Da

Chromosome Location

Chromosome: 14; NC_000014.8 (35179588..35184029, complement). Location: 14q12

3D Structure

ModBase 3D Structure for Q549N0

Immunogen

Synthetic peptide corresponding to aa1-10 (MA[pS]GVTVNDE-C) of human phospho-Cofilin 2, with a C-terminal cysteine added for conjugation purposes, conjugated to KLH. Species Sequence Homology: chicken, mouse; 100%.

Species Sequence Homology

Chicken, mouse; 100%.

Product Categories/Family for anti-CFL2 antibody

Antibodies; Abs to Actin Related Proteins

Applications Tested/Suitable for anti-CFL2 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-CFL2 antibody

Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1-4ug/ml detects phospho-Cofilin 2 in RIPA lysates from L6 cells, treated with LIM Kinase 1, active, Recombinant for 30 minutes. Cofilin 2 is phosphorylated at Ser3 by LIM Kinase. 500ug L6 lysate was incubated with or without 100mU LIM Kinase at 30 degree C for 30 minutes. Phosphorylation was terminated by boiling in reducing sample buffer. Aliquots (10ug) were resolved by electrophoresis and subjected to Western Blot, using 1ug/ml C7506-51. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.

Other Notes

Small volumes of anti-CFL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CFL2 purchase

MBS624852 is an antibody targeting CFL2. Also known as Cofilin 2, phosphorylated (Ser3). Reactive in Chicken, Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CFL2. It may not necessarily be applicable to this product.

NCBI GI #

33946278

NCBI GeneID

1073

NCBI Accession #

NP_619579.1

UniProt Primary Accession #

Q549N0

UniProt Related Accession #

Q549N0; Q9Y281

GenBank Nucleotide #

NM_138638.4

NCBI Official Full Name

cofilin-2

NCBI Official Synonym Full Names

cofilin 2 (muscle)

NCBI Official Symbol

CFL2

NCBI Official Synonym Symbols

NEM7

UniProt Protein Name

Cofilin 2 (Muscle), isoform CRA_a

Protein Family

Cofilin

UniProt Gene Name

CFL2

UniProt Entry Name

Q549N0_HUMAN

NCBI Protein Information

cofilin-2; OTTHUMP00000178805; OTTHUMP00000178806; cofilin, muscle isoform

NCBI Summary for CFL2

This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

UniProt Comments for CFL2

Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Cytoskeletal; Nuclear receptor co-regulator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: I band; extracellular space; nuclear matrix; actin cytoskeleton

Molecular Function: protein binding; actin binding

Biological Process: muscle maintenance; actin filament depolymerization; positive regulation of actin filament depolymerization; sarcomere organization

Disease: Nemaline Myopathy 7

Research Articles on CFL2

1. PHD2 affects cell migration and F-actin formation via RhoA/rho-associated kinase-dependent cofilin phosphorylation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CFL2 antibody

Reference Product	PubMed Publications
LIMK1 antibody	>1 publications with CFL2 and LIMK1

Pathways associated with anti-CFL2 antibody

Products by Pathway	Pathway Diagram
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Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
G13 Signaling Pathway antibodies	G13 Signaling Pathway Diagram
Pertussis Pathway antibodies	Pertussis Pathway Diagram
Pertussis Pathway antibodies	Pertussis Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram

Diseases associated with anti-CFL2 antibody

Disease Name	Pubmed Publications
Myopathies, Nemaline Antibodies	>3 publications with CFL2 and Myopathies, Nemaline
Congenital Abnormalities Antibodies	>2 publications with CFL2 and Congenital Abnormalities
Neoplasms Antibodies	>1 publications with CFL2 and Neoplasms
Anemia Antibodies	>1 publications with CFL2 and Anemia
Neural Tube Defects Antibodies	>1 publications with CFL2 and Neural Tube Defects
Nervous System Malformations Antibodies	>1 publications with CFL2 and Nervous System Malformations
Hyperplasia Antibodies	>1 publications with CFL2 and Hyperplasia
Vascular Diseases Antibodies	>1 publications with CFL2 and Vascular Diseases

Organs/Tissues associated with anti-CFL2 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>15 publications with CFL2 and Muscle
Blood Antibodies	>3 publications with CFL2 and Blood
Vascular Antibodies	>3 publications with CFL2 and Vascular
Heart Antibodies	>3 publications with CFL2 and Heart
Embryonic Tissue Antibodies	>2 publications with CFL2 and Embryonic Tissue
Adipose Tissue Antibodies	>1 publications with CFL2 and Adipose Tissue
Testis Antibodies	>1 publications with CFL2 and Testis