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EMX1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS624908

EMX1 is a transcription factor, which in cooperation with EMX2. It acts to generate the boundary between the roof and archipallium in the developing brain. It may function in combinations with OTX1/2 to specify cell fates in the developing central nervous… More details

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Product Overview

Online Datasheet in PDF
Product Name
EMX1, Polyclonal Antibody
Full Product Name
EMX1, CT (Empty Spiracles-like Protein 1, Empty Spiracles Homolog 1, Homeobox Protein EMX1)
Product Gene Name
Product Synonym Names
Anti -EMX1, CT (Empty Spiracles-like Protein 1, Empty Spiracles Homolog 1, Homeobox Protein EMX1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human EMX1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
28,039 Da
OMIM
600034
3D Structure
ModBase 3D Structure for Q04741
Immunogen
Synthetic peptide selected from the C-terminal region of human EMX1 (KLH).
Applications Tested/Suitable for anti-EMX1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-EMX1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500

Western Blot (WB) of anti-EMX1 antibody

Western Blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane) using MBS624908. This demonstrates that MBS624908 detected the EMX1 protein (arrow).
Rabbit Anti-EMX1 Polyclonal Antibody – Cat# MBS624908

Flow Cytometry (FC/FACS) of anti-EMX1 antibody

Flow Cytometric analysis of MDA-MB231 cells using MBS624908 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-EMX1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS624908

Immunofluorescence (IF) of anti-EMX1 antibody

Fluorescent confocal image of Hela cell stained with MBS624908. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBS624908 (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1h at 37℃). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10 min). EMX1 immunoreactivity is localized to Nucleus significantly.
Rabbit Anti-EMX1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS624908

Other Notes

Small volumes of anti-EMX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EMX1 purchase

MBS624908 is an antibody targeting EMX1. Also known as EMX1, CT (Empty Spiracles-like Protein 1, Empty Spiracles Homolog 1, Homeobox Protein EMX1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EMX1. It may not necessarily be applicable to this product.

NCBI GI #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
EMX1
UniProt Protein Name
Homeobox protein EMX1
UniProt Synonym Protein Names
Empty spiracles homolog 1; Empty spiracles-like protein 1
Protein Family
UniProt Gene Name
EMX1
UniProt Entry Name
EMX1_HUMAN

UniProt Comments for EMX1

EMX1: Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system. Belongs to the EMX homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p13.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: response to drug; regulation of transcription, DNA-dependent; in utero embryonic development; brain morphogenesis; cerebral cortex regionalization; post-embryonic development; cerebral cortex neuron differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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