Rabbit Anti-DIDO1 Polyclonal Antibody – Cat# MBS625040

Western blot analysis of DIO1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). DIO1 (arrow) was detected using the purified Pab.

DIDO1 Antibody – Rabbit Polyclonal

Name: DIDO1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS625040
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625040

DIO1 is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner… More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DIO1 (DIDO1), Polyclonal Antibody

Full Product Name

DIO1, NT (Type I Iodothyronine Deiodinase, Type-I 5'-deiodinase, Type 1 DI, DIOI, 5DI, TXDI1, ITDI1)

Product Gene Name

anti-DIDO1 antibody

Product Synonym Names

Anti -DIO1, NT (Type I Iodothyronine Deiodinase, Type-I 5'-deiodinase, Type 1 DI, DIOI, 5DI, TXDI1, ITDI1)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A and peptide affinity chromatography.

Specificity

Recognizes human DIO1 at ~28kD.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

243,873 Da

Chromosome Location

Chromosome: 20; NC_000020.10 (61509090..61569304, complement). Location: 20q13.33

OMIM

604140

3D Structure

ModBase 3D Structure for Q9BTC0

Immunogen

KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa36-66 of the N-terminal region of human DIO1.

Product Categories/Family for anti-DIDO1 antibody

Antibodies; Abs to Apoptosis

Applications Tested/Suitable for anti-DIDO1 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-DIDO1 antibody

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1000
Western Blot: 1:100-1:500

Other Notes

Small volumes of anti-DIDO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DIDO1 purchase

MBS625040 is an antibody targeting DIDO1. Also known as DIO1, NT (Type I Iodothyronine Deiodinase, Type-I 5'-deiodinase, Type 1 DI, DIOI, 5DI, TXDI1, ITDI1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DIDO1. It may not necessarily be applicable to this product.

NCBI GI #

301129172

NCBI GeneID

11083

NCBI Accession #

NP_001180299.1

UniProt Primary Accession #

Q9BTC0

UniProt Secondary Accession #

O15043; Q3ZTL7; Q3ZTL8; Q4VXS1; Q4VXS2; Q4VXV8; Q4VXV9; Q96D72; A8MY65; B9EH82; E1P5I1

UniProt Related Accession #

Q9BTC0

GenBank Nucleotide #

NM_001193370.1

NCBI Official Full Name

death-inducer obliterator 1 isoform b

NCBI Official Synonym Full Names

death inducer-obliterator 1

NCBI Official Symbol

DIDO1

NCBI Official Synonym Symbols

BYE1; DIO1; DATF1; DIDO2; DIDO3; DIO-1; FLJ11265; KIAA0333; MGC16140; C20orf158; dJ885L7.8; DKFZp434P1115

UniProt Protein Name

Death-inducer obliterator 1

UniProt Synonym Protein Names

Death-associated transcription factor 1

Protein Family

Death-inducer obliterator

UniProt Gene Name

DIDO1

UniProt Synonym Gene Names

C20orf158; DATF1; KIAA0333

UniProt Entry Name

DIDO1_HUMAN

NCBI Protein Information

death-inducer obliterator 1; DATF-1; hDido1; OTTHUMP00000031517; OTTHUMP00000031518; OTTHUMP00000031519; OTTHUMP00000031520; OTTHUMP00000031521; OTTHUMP00000031522; death-associated transcription factor 1

NCBI Summary for DIDO1

Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms. [provided by RefSeq]

UniProt Comments for DIDO1

DATF1: Putative transcription factor, weakly pro-apoptotic when overexpressed. Tumor suppressor. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: cytoplasm; spindle; nucleus

Molecular Function: zinc ion binding

Biological Process: transcription, DNA-dependent

Research Articles on DIDO1

1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DIDO1 antibody

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Chromosome Maintenance Pathway antibodies	Chromosome Maintenance Pathway Diagram
Meiosis Pathway antibodies	Meiosis Pathway Diagram
Meiotic Synapsis Pathway antibodies	Meiotic Synapsis Pathway Diagram

Diseases associated with anti-DIDO1 antibody

Disease Name	Pubmed Publications
Chromosome Aberrations Antibodies	>2 publications with DIDO1 and Chromosome Aberrations
Breast Neoplasms Antibodies	>1 publications with DIDO1 and Breast Neoplasms
Necrosis Antibodies	>1 publications with DIDO1 and Necrosis

Organs/Tissues associated with anti-DIDO1 antibody

Organ/Tissue Name	Pubmed Publications
Bone Marrow Antibodies	>2 publications with DIDO1 and Bone Marrow
Bone Antibodies	>2 publications with DIDO1 and Bone
Thyroid Antibodies	>1 publications with DIDO1 and Thyroid
Prostate Antibodies	>1 publications with DIDO1 and Prostate
Spleen Antibodies	>1 publications with DIDO1 and Spleen
Liver Antibodies	>1 publications with DIDO1 and Liver
Blood Antibodies	>1 publications with DIDO1 and Blood
Brain Antibodies	>1 publications with DIDO1 and Brain