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CD14 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6251438

MBS6251438 is an antibody targeting CD14. Also known as CD14 (PE). Reactive in Human, Porcine. Host: Mouse. More details

$1170 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD14, Monoclonal Antibody
Full Product Name
CD14 (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
MIL2
Host
Mouse
Species Reactivity
Human, Porcine
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes porcine CD14. Species Crossreactivity: human. Demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry. Lymphocytes and eosinophils are negative for staining.
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
3D Structure
ModBase 3D Structure for A2SW51
Immunogen
Porcine peripheral blood lymphocytes
Conjugate
PE

Product Categories/Family for anti-CD14 antibody

Applications Tested/Suitable for anti-CD14 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Testing Data of anti-CD14 antibody

Staining of pig peripheral blood granulocytes with 227403 followed by IgG (FITC) goat anti mouse
Mouse Anti-CD14 Monoclonal Antibody – Cat# MBS6251438

Product References and Citations for anti-CD14 antibody

1. Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues. J. Imm. Methods. 170: 233-245. 2. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers. Vet. Immunol. Immunopathol. 80: 93-109. 3. Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs. FASEB J. 24: 712-22. 4. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys. Kidney Int. 58: 838-50. 5. Li, Y. et al. (2014) Identification of apoptotic cells in the thymus of piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus. Virus Res. pii: S0168-1702(14)00173-7. 6. Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon- producing cells. Immunology. 110: 440-9. 7. Vanderheijden, N. et al. (2003) Involvement of sialoadhesin in entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages. J Virol. 77: 8207-15. 8. Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans. J Immunol. 187: 4913-9. 9. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys. Kidney Int. 62: 654-67. 10. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide. J Immunol. 188: 3382-94. 11. Thorgersen, E.B. et al. (2009) Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood. Infect Immun. 77: 725-32. 12. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining. J Immunol Methods. 327: 18-29. 13. Facci, M.R. et al. (2011) Stability of expression of reference genes in porcine peripheral blood mononuclear and dendritic cells. Vet Immunol Immunopathol. 141: 11-5. 14. Koutna', I. et al. (2012) Flow Cytometry Analysis of Intracellular Protein In: Flow Cytometry - Recent Perspectives, Schmid, I. (Ed.), ISBN: 978-953-51- 15. Facci, M.R. et al. (2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs. Immunology. 129: 396-405. 16. Schierack, P. et al. (2009) Effects of Bacillus cereus var. toyoi on immune parameters of pregnant sows. Vet Immunol Immunopathol.127: 26-37. 17. Lundeland, B. et al. (2011) Severe gunshot injuries in a porcine model: impact on central markers of innate immunity. Acta Anaesthesiol Scand. 55: 28-34. 18. Thorgersen, E.B. et al. (2008) Cyanobacterial LPS antagonist (CyP)-a novel and efficient inhibitor of Escherichia coli LPS-induced cytokine response in the pig. Mol Immunol. 45: 3553-7. 19. Schierack, P. et al. (2007) Bacillus cereus var. toyoi enhanced systemic immune response in piglets. Vet Immunol Immunopathol. 118: 1-11. 20. Ondrackova, P. et al. (2012) Interaction of porcine neutrophils with different strains of enterotoxigenic Escherichia coli. Vet Microbiol. 160: 108-16. 21. Ondrackova, P. et al. (2012) Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and Salmonella-infected piglets by seven-colour flow cytometry. Res Vet Sci. pii: S0034-5288(12)00256-1. 22. Vicenova, M. et al. (2014) Evaluation of in vitro and in vivo anti-inflammatory activity of biologically active phospholipids with anti-neoplastic potential in porcine model. BMC Complement Altern Med. 14: 339.

Other Notes

Small volumes of anti-CD14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD14. It may not necessarily be applicable to this product.

UniProt Primary Accession #
UniProt Protein Name
Monocyte differentiation antigen CD14
UniProt Synonym Protein Names
Myeloid cell-specific leucine-rich glycoprotein PIRNR PIRNR002017
UniProt Gene Name
CD14
UniProt Entry Name
A2SW51_PIG

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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