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CD14 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6251441

CD14 is found chiefly on monocytes. UCHM1 was clustered at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens, Oxford 1986, as CDw14, group A. More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD14, Monoclonal Antibody
Full Product Name
CD14 (CD14 Antigen, Lipopolysaccharide Receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid Cell Specific Leucine Rich Glycoprotein) (PE)
Product Gene Name
Product Synonym Names
CD14 antigen; Lipopolysaccharide receptor; LPSR; Monocyte Differentiation Antigen 14; Myeloid cell specific leucine rich glycoprotein
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3H493 (UCHM1)
Host
Mouse
Species Reactivity
Human, Monkey
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified by Protein A affinity chromatography from tissue culture supernatant.
Concentration
1mg/ml (lot specific)
Specificity
Recognizes human CD14. Recognizes a monocyte surface antigen of 55kD found chiefly on monocytes. Gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells in tonsil tissue sections. Skin Langerhans cells are always negative. Also reacts with Kupffer cells and sinus lining cells on the liver. Species Crossreactivity: Cynomolgus monkey, Rhesus monkey, fish, trout.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
3D Structure
ModBase 3D Structure for P08571
Immunogen
Human Thymocytes followed by peripheral blood mononuclear cells.
Hybridoma
NS1-Ag4/1myeloma cells with human thymocytes and PBLs
Immunohistochemistry Positive Control
Tonsil

Product Categories/Family for anti-CD14 antibody

Applications Tested/Suitable for anti-CD14 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-CD14 antibody
Immunohistochemistry (Frozen): The epitope recognized by this antibody is sensitive to formaldehyde fixation and tissue processing. Acetone fixation for frozen sections is recommended. Not suitable for use with paraffin sections.
Optimal dilutions to be determined by the researcher.

Testing Data of anti-CD14 antibody

Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD14-IgG:AZIDE FREE
Mouse Anti-CD14 Monoclonal Antibody – Cat# MBS6251441

Product References and Citations for anti-CD14 antibody

1. Hogg, N. et al. (1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunol. 53: 753-768, 2. Linch, D.C. et al. (1984) Monoclonal antibodies differentiation between monocyte and non-monocytic varients of AML. Blood. 63: 566-573, 3. Hogg, N. and Horton, M.A. (1987) Myeloid antigens: new and previously defined clusters in Leucocyte Typing III White Cell differentiation antigens. Edited by McMichael, A.J., Beverley, P.C.L., Cobbold, S., Crumpton, M.J., Gilks, W., Gotch, F.M., Hogg, N., Horton, M., Ling, N., Mackenna, I.C.M., Mason, D.Y., Milstein, G., Speghelhalter., D. and Waldmann, H. et al, 4. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes. Leucocyte Typing IV. Oxford University Press p 1058-1063, 5. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97-110. 6. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands. J. Immunol. 176: 5730-5734.7. Bournazos, S. et al. (2008) Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status. Arterioscler Thromb Vasc Biol. 28: 1491-8 8. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 12:2. 9. Hsu, T.L. et al. (2002) Modulation of dendritic cell differentiation and maturation by decoy receptor 3. J Immunol. 168: 4846-53. 10. Karlsson, H. et al. (2002) Innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora. Infect Immun. 70: 6688-96.

Other Notes

Small volumes of anti-CD14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD14 purchase

MBS6251441 is an antibody targeting CD14. Also known as CD14 (CD14 Antigen, Lipopolysaccharide Receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid Cell Specific Leucine Rich Glycoprotein) (PE). Reactive in Human, Monkey. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD14. It may not necessarily be applicable to this product.

UniProt Primary Accession #

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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