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ESRRA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625160

ESRRA is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact.… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ESRRA, Polyclonal Antibody
Full Product Name
ESRRA, ID (Steroid Hormone Receptor ERR1, Estrogen-related Receptor, alpha, ERR-alpha, Estrogen Receptor-like 1, Nuclear Receptor Subfamily 3 Group B Member 1, NR3B1, ESRL1, ERR1)
Product Gene Name
Product Synonym Names
Anti -ESRRA, ID (Steroid Hormone Receptor ERR1, Estrogen-related Receptor, alpha, ERR-alpha, Estrogen Receptor-like 1, Nuclear Receptor Subfamily 3 Group B Member 1, NR3B1, ESRL1, ERR1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ESRRA.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
12,276 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (64073044..64084210). Location: 11q13
3D Structure
ModBase 3D Structure for Q6GMQ8
Immunogen
Synthetic peptide selected from the Center region of human ESRRA (KLH).
Applications Tested/Suitable for anti-ESRRA antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-ESRRA antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500

Western Blot (WB) of anti-ESRRA antibody

Western blot analysis of ESRRA Antibody (Center) in A375 cell line lysates (35ug/lane). ESRRA (arrow) was detected using the purified Pab.
Rabbit Anti-ESRRA Polyclonal Antibody – Cat# MBS625160

Immunohistochemistry (IHC) of anti-ESRRA antibody

Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ESRRA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS625160

Flow Cytometry (FC/FACS) of anti-ESRRA antibody

Flow cytometric analysis of 293 cells using ESRRA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ESRRA Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS625160

Western Blot (WB) of anti-ESRRA antibody

Western blot analysis of ESRRA (arrow) using rabbit polyclonal ESRRA Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ESRRA gene.
Rabbit Anti-ESRRA Polyclonal Antibody, Western Blot (WB) – Cat# MBS625160

Immunofluorescence (IF) of anti-ESRRA antibody

Confocal immunofluorescent analysis of ESRRA Antibody (Center) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-ESRRA Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS625160

Other Notes

Small volumes of anti-ESRRA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ESRRA purchase

MBS625160 is an antibody targeting ESRRA. Also known as ESRRA, ID (Steroid Hormone Receptor ERR1, Estrogen-related Receptor, alpha, ERR-alpha, Estrogen Receptor-like 1, Nuclear Receptor Subfamily 3 Group B Member 1, NR3B1, ESRL1, ERR1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ESRRA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
ESRRA protein
NCBI Official Synonym Full Names
estrogen-related receptor alpha
NCBI Official Symbol
ESRRA
NCBI Official Synonym Symbols
ERR1; ERRa; ESRL1; NR3B1; ERRalpha
UniProt Protein Name
ESRRA protein
UniProt Gene Name
ESRRA
UniProt Entry Name
Q6GMQ8_HUMAN
NCBI Protein Information
steroid hormone receptor ERR1; ERR-alpha; OTTHUMP00000198089; OTTHUMP00000198090; OTTHUMP00000198091; OTTHUMP00000237048; estrogen receptor-like 1; estrogen-related nuclear receptor alpha; nuclear receptor subfamily 3 group B member 1

NCBI Summary for ESRRA

The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. [provided by RefSeq]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ESRRA antibody

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