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ENPP2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625167

ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses… More details

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Product Overview

Online Datasheet in PDF
Product Name
ENPP2, Polyclonal Antibody
Full Product Name
ENPP2, ID (K416) (Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2, E-NPP 2, Extracellular Lysophospholipase D, LysoPLD, Autotaxin, ATX, PDNP2)
Product Gene Name
anti-Enpp2 antibody
Product Synonym Names
Anti -ENPP2, ID (K416) (Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2, E-NPP 2, Extracellular Lysophospholipase D, LysoPLD, Autotaxin, ATX, PDNP2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ENPP2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
101,576 Da
Chromosome Location
Chromosome: 7; NC_005106.3 (95120407..95202176, complement). Location: 7q31
3D Structure
ModBase 3D Structure for Q64610
Immunogen
Synthetic peptide selected from the Center region of human ENPP2 (KLH).

Product Categories/Family for anti-Enpp2 antibody

Antibodies; Abs to Enzymes; Phosphatase
Applications Tested/Suitable for anti-Enpp2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Enpp2 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-Enpp2 antibody

Western blot analysis of anti-ENPP2 Antibody (Center K416) in Y79 cell line lysates (35ug/lane).ENPP2(arrow) was detected using the purified Pab.
Rabbit Anti-ENPP2 Polyclonal Antibody – Cat# MBS625167

Western Blot (WB) of anti-Enpp2 antibody

Western blot analysis of anti-ENPP2 Antibody (Center K416) in mouse cerebellum tissue lysates (35ug/lane).ENPP2(arrow) was detected using the purified Pab.
Rabbit Anti-ENPP2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS625167

Immunohistochemistry (IHC) of anti-Enpp2 antibody

ENPP2 Antibody (Center K416) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENPP2 Antibody (Center K416) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-ENPP2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS625167

Flow Cytometry (FC/FACS) of anti-Enpp2 antibody

ENPP2 Antibody (Center K416) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ENPP2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS625167

Other Notes

Small volumes of anti-Enpp2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Enpp2 purchase

MBS625167 is an antibody targeting Enpp2. Also known as ENPP2, ID (K416) (Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2, E-NPP 2, Extracellular Lysophospholipase D, LysoPLD, Autotaxin, ATX, PDNP2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Enpp2. It may not necessarily be applicable to this product.

NCBI GI #
86439949
NCBI GeneID
84050
NCBI Accession #
NP_476445.2
UniProt Primary Accession #
Q64610
UniProt Secondary Accession #
Q66HQ0
UniProt Related Accession #
Q64610
GenBank Nucleotide #
NM_057104.2
NCBI Official Full Name
ectonucleotide pyrophosphatase/phosphodiesterase family member 2
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 2
NCBI Official Symbol
Enpp2
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
UniProt Synonym Protein Names
Autotaxin; Extracellular lysophospholipase D
Protein Family
Ectonucleotide pyrophosphatase/phosphodiesterase family
UniProt Gene Name
Enpp2
UniProt Synonym Gene Names
Atx; Npps2; E-NPP 2; LysoPLD
UniProt Entry Name
ENPP2_RAT
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; lysoPLD; autotaxin; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2 (autotaxin)

NCBI Summary for Enpp2

may regulate oligodendrocyte cell-extracellular matrix adhesive interactions during myelination [RGD, Feb 2006]

UniProt Comments for Enpp2

Function: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Ref.4 Ref.6

Catalytic activity: 1-alkyl-sn-glycero-3-phosphoethanolamine + H2O = 1-alkyl-sn-glycerol 3-phosphate + ethanolamine. Ref.7

Cofactor: Binds 2 zinc ions per subunit. Ref.7Binds 1 calcium ion per subunit. Ref.7

Enzyme regulation: Inhibited by lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P). Inhibited by EDTA and EGTA

By similarity.

Subcellular location: Secreted. Note: Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells. Ref.2 Ref.4 Ref.5 Ref.6 Ref.7

Tissue specificity: Abundantly expressed in cerebrum and cerebellum. Localized in secretory epithelial cells in the brain and the eye including choroid plexus epithelial cells, ciliary epithelial cells, iris pigment epithelial cells, and retinal pigment cells. Ref.1 Ref.2

Post-translational modification: N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity

By similarity.It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.

Sequence similarities: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.Contains 2 SMB (somatomedin-B) domains.
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Research Articles on Enpp2

1. A physical interaction between autotaxin (ATX) and P2Y12 receptor provides the basis for an ATX-MORFO-P2Y12 signaling axis crucial for cellular function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Enpp2 antibody

 Reference Product  PubMed Publications
 Enpp6 antibody  >1 publications with Enpp2 and Enpp6

Pathways associated with anti-Enpp2 antibody

 Products by Pathway  Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram

Diseases associated with anti-Enpp2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >14 publications with Enpp2 and Neoplasms
 Inflammation Antibodies  >9 publications with Enpp2 and Inflammation
 Fibrosis Antibodies  >7 publications with Enpp2 and Fibrosis
 Breast Neoplasms Antibodies  >6 publications with Enpp2 and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >4 publications with Enpp2 and Neoplasm Metastasis
 Lung Neoplasms Antibodies  >3 publications with Enpp2 and Lung Neoplasms
 Cardiovascular Diseases Antibodies  >3 publications with Enpp2 and Cardiovascular Diseases
 Necrosis Antibodies  >3 publications with Enpp2 and Necrosis
 Hepatitis Antibodies  >3 publications with Enpp2 and Hepatitis
 Nervous System Diseases Antibodies  >2 publications with Enpp2 and Nervous System Diseases

Organs/Tissues associated with anti-Enpp2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with Enpp2 and Brain
 Lung Antibodies  >6 publications with Enpp2 and Lung
 Liver Antibodies  >2 publications with Enpp2 and Liver
 Heart Antibodies  >2 publications with Enpp2 and Heart
 Eye Antibodies  >1 publications with Enpp2 and Eye
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