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LEO1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625172

The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. It may be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of… More details

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Product Overview

Online Datasheet in PDF
Product Name
LEO1, Polyclonal Antibody
Full Product Name
LEO1, NT (RDL, RNA Polymerase-associated Protein LEO1, Replicative Senescence Down-regulated leo1-like Protein, RDL)
Product Gene Name
anti-LEO1 antibody
Product Synonym Names
Anti -LEO1, NT (RDL, RNA Polymerase-associated Protein LEO1, Replicative Senescence Down-regulated leo1-like Protein, RDL)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human LEO1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
53,920 Da
3D Structure
ModBase 3D Structure for P38439
Immunogen
Synthetic peptide selected from the N-terminal region of human LEO1 (KLH).

Product Categories/Family for anti-LEO1 antibody

Antibodies; Abs to Enzymes; Polymerase
Applications Tested/Suitable for anti-LEO1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-LEO1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:250

Western Blot (WB) of anti-LEO1 antibody

Western blot analysis of anti-Leo1 Antibody (N-term) in HL60 cell line lysates (35ug/lane). Leo1(arrow) was detected using the purified Pab.
Rabbit Anti-LEO1 Polyclonal Antibody – Cat# MBS625172

Western Blot (WB) of anti-LEO1 antibody

Western blot analysis of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2) (Origene Technologies).
Rabbit Anti-LEO1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS625172

Other Notes

Small volumes of anti-LEO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LEO1 purchase

MBS625172 is an antibody targeting LEO1. Also known as LEO1, NT (RDL, RNA Polymerase-associated Protein LEO1, Replicative Senescence Down-regulated leo1-like Protein, RDL). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LEO1. It may not necessarily be applicable to this product.

NCBI GI #
1420322
UniProt Primary Accession #
P38439
UniProt Secondary Accession #
D6W2I1
NCBI Official Full Name
LEO1
UniProt Protein Name
RNA polymerase-associated protein LEO1
Protein Family
RNA polymerase-associated protein
UniProt Gene Name
LEO1
UniProt Entry Name
LEO1_YEAST

UniProt Comments for LEO1

Function: The PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA-binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of RAD6 ubiquitin conjugate and the BRE1 ubiquitin ligase which ubiquitinate 'Lys-126' histone H2B. Activates the SET1 histone methyltransferase complex for methylation of 'Lys-4' of histone H3 and for methylation of 'Lys-73' of histone H3 by DOT1 and 'Lys-36' of histone H3 by SET2. Ref.9 Ref.10

Subunit structure: Component of the PAF1 complex which consists of at least CDC73, CTR9, LEO1, PAF1 and RTF1.

Subcellular location: Nucleus › nucleoplasm Ref.9.

Miscellaneous: Present with 1110 molecules/cell in log phase SD medium. Ref.8

Sequence similarities: Belongs to the LEO1 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LEO1 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with LEO1 and FLNA

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