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CAPZA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625492

CAPZA1 is a member of the F-actin capping protein alpha subunit family. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CAPZA1, Polyclonal Antibody
Full Product Name
CAPZA1, NT (F-actin-capping Protein Subunit alpha-1, CapZ alpha-1)
Product Gene Name
Product Synonym Names
Anti -CAPZA1, NT (F-actin-capping Protein Subunit alpha-1, CapZ alpha-1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human CAPZA1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
32,923 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (113162075..113214241). Location: 1p13.2
OMIM
601580
3D Structure
ModBase 3D Structure for P52907
Immunogen
Synthetic peptide selected from the N-terminal region of human CAPZA1 (KLH).
Applications Tested/Suitable for anti-CAPZA1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-CAPZA1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-CAPZA1 antibody

Western blot analysis of CAPZA1 antibody (N-term) in HL60 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.
Rabbit Anti-CAPZA1 Polyclonal Antibody – Cat# MBS625492

Immunohistochemistry (IHC) of anti-CAPZA1 antibody

Immunofluorescence analysis of CAPZA1 Antibody (N-term) with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Rabbit Anti-CAPZA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS625492

Flow Cytometry (FC/FACS) of anti-CAPZA1 antibody

CAPZA1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CAPZA1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS625492

Other Notes

Small volumes of anti-CAPZA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CAPZA1 purchase

MBS625492 is an antibody targeting CAPZA1. Also known as CAPZA1, NT (F-actin-capping Protein Subunit alpha-1, CapZ alpha-1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CAPZA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
CAPZA1
NCBI Official Synonym Full Names
capping protein (actin filament) muscle Z-line, alpha 1
NCBI Official Symbol
CAPZA1
NCBI Official Synonym Symbols
CAPZ; CAZ1; CAPPA1
UniProt Protein Name
F-actin-capping protein subunit alpha-1
UniProt Synonym Protein Names
CapZ alpha-1
UniProt Gene Name
CAPZA1
UniProt Entry Name
CAZA1_HUMAN
NCBI Protein Information
F-actin-capping protein subunit alpha-1; Cap Z; capZ alpha-1; OTTHUMP00000013512

NCBI Summary for CAPZA1

CAPZA1 is a member of the F-actin capping protein alpha subunit family. This gene encodes the alpha subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments. [provided by RefSeq]

UniProt Comments for CAPZA1

CAPZA1: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Belongs to the F-actin-capping protein alpha subunit family.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: F-actin capping protein complex; cytoskeleton; extracellular region; cytosol; actin cytoskeleton

Molecular Function: protein binding; actin binding

Biological Process: innate immune response; protein complex assembly; blood coagulation; cell motility; barbed-end actin filament capping
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CAPZA1 antibody

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