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Ovalbumin Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6255193

MBS6255193 is an antibody targeting Ovalbumin. Also known as Ovalbumin (MaxLight 550). Host: Mouse. More details

$1080 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ovalbumin, Monoclonal Antibody
Full Product Name
Ovalbumin (MaxLight 550)
Clonality
Monoclonal
Isotype
IgE
Clone Number
10F254
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight550.
Purity/Purification
Purified from tissue culture supernatant.
Specificity
Recognizes ovalbumin (OVA). Suitable for use as a mouse IgE standard in ELISA assays.
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Immunogen
Ovalbumin
Conjugate
MaxLight550

Product Categories/Family for anti-Ovalbumin antibody

Applications Tested/Suitable for anti-Ovalbumin antibody
FLISA

ELISA (EIA) of anti-Ovalbumin antibody

O8075-07A used to generate a standard curve for murine IgE in a sandwich ELISA detected using biotin conjugated ovalbumin and streptavidin. Analysis of OVA-specific IgE. The levels of IgE in sera obtained before, during, and after ASIT were detected and quantified by ELISA. The results are presented as mean IgE concentration (mean±SE, n = 8 for each). AD without ASIT: OVA-sensitized untreated mice; ASIT with OVA: OVA-sensitized mice treated by ASIT with OVA; ASIT with sOVA: OVA-sensitized mice treated by ASIT with sOVA; placebo: PBS-sensitized mice. (*, p<0.05).
Mouse Anti-Ovalbumin Monoclonal Antibody – Cat# MBS6255193

ELISA (EIA) of anti-Ovalbumin antibody

O8075-07A used in an ELISA assay for the measurement of ovalbumin specific IgG in an experimental mouse model of airway inflammation. Basal and OVA-specific IgE levels are increased in p110y/d-/- mice. Total and OVA-specific IgE was determined in sera of PBS-treated and OVA-treated KO and corresponding WT mice using ELISA. (A) Total serum IgE. (B) OVA-specific serum IgE. Bars express means + SD; n = 4-8 pooled from three independent experiments. Data were analysed by One-way ANOVA followed by Bonferroni's comparison tests for selected pairs of columns; **P<0.01, *P<0.05.
Mouse Anti-Ovalbumin Monoclonal Antibody, ELISA (EIA) – Cat# MBS6255193

Product References and Citations for anti-Ovalbumin antibody

1. Stevens, T. et al. (2008) Increased transcription of immune and metabolic pathways in naive and allergic mice exposed to diesel exhaust. Toxicol Sci. 102: 359-70. 2. Fairley,K.J. et al. (2007) Exposure to the immunosuppressant, perfluorooctanoic acid, enhances the murine IgE and airway hyperreactivity response to ovalbumin. Toxicol.Sci. 97: 375-83 3. Ellertsen,L.K. et al. (2010) Maternal allergen immunisation to prevent sensitisation in offspring: Th2-polarising adjuvants are more efficient than a Th1-polarising adjuvant in mice. BMC Immunol. 11: 8-174. Kambayashi, T. et al. (2008) Indirect involvement of allergen-captured mast cells in antigen presentation. Blood. 111:1489-96. 5. Paliwal, S. et al. (2010) One-step acquisition of functional biomolecules from tissues. Proc Natl Acad Sci U S A. 107: 14627-32. 6. Chida, Y. et al. (2007) Early-life psychological stress exacerbates adult mouse asthma via the hypothalamus-pituitary-adrenal axis. Am J Respir Crit Care Med. 175: 316-22. 7. Suzaki, Y. et al. (2007) A small-molecule compound targeting CCR5 and CXCR3 prevents airway hyperresponsiveness and inflammation. Eur Respir J. 31: 783-9. 8. Suzaki, Y. et al. (2005) A potent antiangiogenic factor, endostatin prevents the development of asthma in a murine model. J Allergy Clin Immunol. 116 (6): 1220-7. 9. Hansen, J.S. et al. (2011) Determinants of experimental allergic responses: interactions between allergen dose, sex and age. Scand J Immunol. 73 (6): 554-67. 10. Nygaard, U.C. et al. (2015) Early life exposure to bisphenol A investigated in mouse models of airway allergy, food allergy and oral tolerance. Food Chem Toxicol. 83: 17-25. 11. Shershakova N et al. (2015) Allergen-Specific Immunotherapy with Monomeric Allergoid in a Mouse Model of Atopic Dermatitis. PLoS One. 10 (8): e0135070. 13. Diesner, S.C. et al. (2016) A distinct microbiota composition is associated with protection from food allergy in an oral mouse immunization model. Clin Immunol. pii: S1521-6616(16)30300-X. 14. Cheung DS et al. (2010) Development of atopy by severe paramyxoviral infection in a mouse model. Ann Allergy Asthma Immunol. 105 (6): 437-443.e1. 15. Garbani, M. et al. (2016) Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice. Allergy. Sep 3. 16. Mothes, B. et al. (2016) p110y/d Double-Deficiency Induces Eosinophilia and IgE Production but Protects from OVA-Induced Airway Inflammation. PLoS One. 11 (7): e0159310. 18. Andreassen, M. et al. (2015) Cry1Ab protein from Bacillus thuringiensis and MON810 cry1Ab-transgenic maize exerts no adjuvant effect after airway exposure. Scand J Immunol. 81 (3): 192-200. 19. Hamada. K, et al. (2003) Allergen-independent maternal transmission of asthma susceptibility. J. Immunol. 170: 1683-1689.

Other Notes

Small volumes of anti-Ovalbumin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Ovalbumin. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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