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NEDD8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625548

NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to… More details

$895 / 0.2 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
NEDD8, Polyclonal Antibody
Full Product Name
NEDD8, NT (Ubiquitin-like Protein NEDD8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 8, NEDD-8)
Product Gene Name
anti-NEDD8 antibody
Product Synonym Names
Anti -NEDD8, NT (Ubiquitin-like Protein NEDD8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 8, NEDD-8)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human NEDD8.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
9,072 Da
OMIM
603171
3D Structure
ModBase 3D Structure for Q15843
Immunogen
Synthetic peptide selected from the N-terminal region of human NEDD8 (KLH).

Product Categories/Family for anti-NEDD8 antibody

Antibodies; Abs to Ubiquitin
Applications Tested/Suitable for anti-NEDD8 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NEDD8 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-NEDD8 antibody

Western blot analysis of NEDD8 polyclonal antibody in HeLa cell lysate. NEDD8 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Rabbit Anti-NEDD8 Polyclonal Antibody – Cat# MBS625548

Immunohistochemistry (IHC) of anti-NEDD8 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-NEDD8 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS625548

Other Notes

Small volumes of anti-NEDD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEDD8 purchase

MBS625548 is an antibody targeting NEDD8. Also known as NEDD8, NT (Ubiquitin-like Protein NEDD8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 8, NEDD-8). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NEDD8. It may not necessarily be applicable to this product.

NCBI GI #
47115261
UniProt Primary Accession #
Q15843
UniProt Secondary Accession #
Q3SXN8; Q6LES6
NCBI Official Full Name
NEDD8
UniProt Protein Name
NEDD8
UniProt Synonym Protein Names
Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
Protein Family
NEDD8
UniProt Gene Name
NEDD8
UniProt Entry Name
NEDD8_HUMAN

UniProt Comments for NEDD8

NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: nucleus; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; protein neddylation; anatomical structure morphogenesis; protein localization; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEDD8 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with NEDD8 and FLNA

Pathways associated with anti-NEDD8 antibody

 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram

Diseases associated with anti-NEDD8 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >30 publications with NEDD8 and Neoplasms
 Nervous System Diseases Antibodies  >14 publications with NEDD8 and Nervous System Diseases
 Carcinoma Antibodies  >11 publications with NEDD8 and Carcinoma
 Kidney Neoplasms Antibodies  >7 publications with NEDD8 and Kidney Neoplasms
 Adenocarcinoma Antibodies  >7 publications with NEDD8 and Adenocarcinoma
 Kidney Diseases Antibodies  >6 publications with NEDD8 and Kidney Diseases
 Leukemia Antibodies  >6 publications with NEDD8 and Leukemia
 Urologic Neoplasms Antibodies  >6 publications with NEDD8 and Urologic Neoplasms
 Carcinoma, Small Cell Antibodies  >5 publications with NEDD8 and Carcinoma, Small Cell
 Inflammation Antibodies  >4 publications with NEDD8 and Inflammation

Organs/Tissues associated with anti-NEDD8 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >14 publications with NEDD8 and Kidney
 Brain Antibodies  >13 publications with NEDD8 and Brain
 Blood Antibodies  >10 publications with NEDD8 and Blood
 Nerve Antibodies  >8 publications with NEDD8 and Nerve
 Lung Antibodies  >6 publications with NEDD8 and Lung
 Heart Antibodies  >5 publications with NEDD8 and Heart
 Testis Antibodies  >5 publications with NEDD8 and Testis
 Vascular Antibodies  >5 publications with NEDD8 and Vascular
 Muscle Antibodies  >5 publications with NEDD8 and Muscle
 Embryonic Tissue Antibodies  >4 publications with NEDD8 and Embryonic Tissue
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