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Tyrosine Hydroxylase (TH) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625642

Tyrosine hydroxylase catalyzes the rate limiting step in the synthesis of dopamine and other catecholamines. The enzyme's activity is regulated at the levels of transcription, translation and post-translational modification. Tyrosine hydroxylase exists in… More details

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Product Overview

Online Datasheet in PDF
Product Name
Tyrosine Hydroxylase (TH), Polyclonal Antibody
Full Product Name
Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)
Product Gene Name
anti-TH antibody
Product Synonym Names
Anti -Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Specificity
Detects endogenous levels of tyrosine hydroxylase only when phosphorylated at serine 40.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
14,058 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (2185159..2193035, complement). Location: 11p15.5
3D Structure
ModBase 3D Structure for O43816
Immunogen
Synthetic phosphopeptide (KLH coupled) around Ser40 of human tyrosine hydroxylase.

Product Categories/Family for anti-TH antibody

Antibodies; Abs to Enzymes
Applications Tested/Suitable for anti-TH antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-TH antibody
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunocytochemistry., , Recommended Dilution:, Western Blot: 1:1000, Immunocytochemistry (IF): 1:200, Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-TH antibody

Western blot analysis of extracts from PC12 cells, untreated or forskolin-treated (30 µM), using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (upper) or Tyrosine Hydroxylase Antibody #T9237-10A (lower).
Rabbit Anti-TH Polyclonal Antibody – Cat# MBS625642

Testing Data of anti-TH antibody

Confocal images of brain cortical secions from rats, mock-treated (control) or subjected to 15 minutes ischemia followed by 24 hours of reperfusion, using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (green) and propidium iodide (red). Phospho-tyrosine hydrolase is decreased during 15 minutes ischemia and returns to control levels after 24 hours of reperfusion. Images provided by Dr. Bingren Hu, University of Miami, School of Medicine, Miami, Florida.
Rabbit Anti-TH Polyclonal Antibody, Testing Data – Cat# MBS625642

Immunofluorescence (IF) of anti-TH antibody

Immunofluorescent staining of rat PC12 cells, untreated (A-C) or forskolin-treated (D-F), using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (A, D), the same antibody preincubated with either the phospho-antigenic peptide (B, E) the corresponding nonphospho-peptide (C, F).
Rabbit Anti-TH Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS625642

Other Notes

Small volumes of anti-TH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TH purchase

MBS625642 is an antibody targeting TH. Also known as Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TH. It may not necessarily be applicable to this product.

NCBI GI #
986909
NCBI GeneID
7054
UniProt Primary Accession #
O43816
UniProt Related Accession #
O43816; P07101; P78428; Q0PWM2; Q0PWM3; Q15587; Q16846; Q2M3B4; Q6XS77; Q7KZ36
NCBI Official Full Name
tyrosine hydroxylase
NCBI Official Synonym Full Names
tyrosine hydroxylase
NCBI Official Symbol
TH
NCBI Official Synonym Symbols
TYH; DYT14; DYT5b
UniProt Protein Name
Tyrosine hydroxylase
UniProt Entry Name
O43816_HUMAN
NCBI Protein Information
tyrosine 3-monooxygenase; dystonia 14; OTTHUMP00000011163; OTTHUMP00000011225; OTTHUMP00000011226; tyrosine 3-hydroxylase

NCBI Summary for TH

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]
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UniProt Comments for TH

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Amino Acid Metabolism - tyrosine; EC 1.14.16.2; Mitochondrial; Endoplasmic reticulum; Vesicle; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: synaptic vesicle; internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; cytoplasm; melanosome membrane; dendrite; terminal button; perikaryon; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; synaptic transmission, dopaminergic; response to peptide hormone stimulus; heart development; response to lipopolysaccharide; locomotory behavior; dopamine biosynthetic process; fatty acid metabolic process; regulation of heart contraction; response to salt stress; phytoalexin metabolic process; response to estradiol stimulus; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; sensory perception of sound; visual perception; response to electrical stimulus; epinephrine biosynthetic process; glycoside metabolic process; response to corticosterone stimulus; response to pyrethroid; neurotransmitter biosynthetic process; response to nutrient levels; response to light stimulus; terpene metabolic process; anatomical structure morphogenesis; sphingolipid metabolic process; eating behavior; response to amphetamine; phthalate metabolic process; mating behavior; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; social behavior; response to herbicide; response to ether; memory; synaptic vesicle amine transport; eye photoreceptor cell development; organ morphogenesis; response to ethanol; circadian sleep/wake cycle; pigmentation; response to zinc ion; response to hypoxia; response to activity; cerebral cortex development; embryonic camera-type eye morphogenesis; response to water deprivation

Disease: Segawa Syndrome, Autosomal Recessive
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Research Articles on TH

1. These results suggest that the phosphorylation of the N-terminal portion of TH regulates the degradation of this enzyme by the ubiquitin-proteasome pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TH antibody

 Reference Product  PubMed Publications
 MAPK1 antibody  >1028 publications with TH and MAPK1
 SNCA antibody  >87 publications with TH and SNCA
 MAPKAPK2 antibody  >48 publications with TH and MAPKAPK2
 MAPK3 antibody  >5 publications with TH and MAPK3
 CAMK2G antibody  >5 publications with TH and CAMK2G
 YWHAZ antibody  >4 publications with TH and YWHAZ
 YWHAB antibody  >4 publications with TH and YWHAB

Pathways associated with anti-TH antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Biosynthesis Of Amine And Peptide Hormones Pathway antibodies  Biosynthesis Of Amine And Peptide Hormones Pathway Diagram
 Catecholamine Biosynthesis Pathway antibodies  Catecholamine Biosynthesis Pathway Diagram
 Dopamine Neurotransmitter Release Cycle Pathway antibodies  Dopamine Neurotransmitter Release Cycle Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Metabolism Of Hormones Pathway antibodies  Metabolism Of Hormones Pathway Diagram

Diseases associated with anti-TH antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1105530 publications with TH and Neoplasms
 Cardiovascular Diseases Antibodies  >883656 publications with TH and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >811276 publications with TH and Nervous System Diseases
 Heart Diseases Antibodies  >447261 publications with TH and Heart Diseases
 Brain Diseases Antibodies  >383626 publications with TH and Brain Diseases
 Mental Disorders Antibodies  >375178 publications with TH and Mental Disorders
 Gastrointestinal Diseases Antibodies  >357618 publications with TH and Gastrointestinal Diseases
 Pain Antibodies  >290199 publications with TH and Pain
 Kidney Diseases Antibodies  >194714 publications with TH and Kidney Diseases
 Myocardial Ischemia Antibodies  >190640 publications with TH and Myocardial Ischemia

Organs/Tissues associated with anti-TH antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >237302 publications with TH and Brain
 Eye Antibodies  >127559 publications with TH and Eye
 Nerve Antibodies  >108344 publications with TH and Nerve
 Mouth Antibodies  >92340 publications with TH and Mouth
 Pancreas Antibodies  >36230 publications with TH and Pancreas
 Adrenal Gland Antibodies  >18173 publications with TH and Adrenal Gland
 Embryonic Tissue Antibodies  >14286 publications with TH and Embryonic Tissue
 Placenta Antibodies  >7810 publications with TH and Placenta
 Uncharacterized Tissue Antibodies  >80 publications with TH and Uncharacterized Tissue
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