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BH3 Domain (BNIP3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625815

NIP3 is a member of the BCL2/adenovirus E1B 19kD-interacting protein (BNIP) family. It interacts with the E1B 19kD protein which is responsible for the protection of virally-induced cell death, as well as E1B 19kD-like sequences of BCL2, also an apoptotic… More details

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Product Overview

Online Datasheet in PDF
Product Name
BNIP3, BH3 Domain, Polyclonal Antibody
Full Product Name
BNIP3, BH3 Domain (NIP3, BCL2/Adenovirus E1B 19kD Protein-interacting Protein 3)
Product Gene Name
anti-BNIP3 antibody
Product Synonym Names
Anti -BNIP3, BH3 Domain (NIP3, BCL2/Adenovirus E1B 19kD Protein-interacting Protein 3)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human BNIP3. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
21,541 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (133781204..133795435, complement). Location: 10q26.3
OMIM
603293
3D Structure
ModBase 3D Structure for Q12983
Immunogen
Synthetic peptide selected from the N-terminal region (BH3 domain) of human NIP3 (KLH).

Product Categories/Family for anti-BNIP3 antibody

Antibodies; Abs to Apoptosis
Applications Tested/Suitable for anti-BNIP3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-BNIP3 antibody
Suitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:50-1:100

Immunofluorescence (IF) of anti-BNIP3 antibody

Fluorescent confocal image of HepG2 cells stained with BNIP3 (BH3 Domain Specific) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with BNIP3 (BH3 Domain Specific) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.
Rabbit Anti-BNIP3 Polyclonal Antibody – Cat# MBS625815

Western Blot (WB) of anti-BNIP3 antibody

The anti-NIP3 BH3 domain Pab is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).
Rabbit Anti-BNIP3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS625815

Immunofluorescence (IF) of anti-BNIP3 antibody

Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody Cat # AP1321a. Data courtesy of Ruben Zamora, University of Pittsburgh.
Rabbit Anti-BNIP3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS625815

Immunohistochemistry (IHC) of anti-BNIP3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-BNIP3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS625815

Other Notes

Small volumes of anti-BNIP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BNIP3 purchase

MBS625815 is an antibody targeting BNIP3. Also known as BNIP3, BH3 Domain (NIP3, BCL2/Adenovirus E1B 19kD Protein-interacting Protein 3). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BNIP3. It may not necessarily be applicable to this product.

NCBI GI #
7669481
NCBI GeneID
664
NCBI Accession #
NP_004043.2
UniProt Primary Accession #
Q12983
UniProt Secondary Accession #
O14620; Q96GP0
UniProt Related Accession #
Q12983; Q53HF4; Q66K24; Q6NVY4
GenBank Nucleotide #
NM_004052.2
NCBI Official Full Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
NCBI Official Synonym Full Names
BCL2/adenovirus E1B 19kDa interacting protein 3
NCBI Official Symbol
BNIP3
NCBI Official Synonym Symbols
NIP3
UniProt Protein Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Protein Family
BCL2/adenovirus E1B 19 kDa protein-interacting protein
UniProt Gene Name
BNIP3
UniProt Synonym Gene Names
NIP3
UniProt Entry Name
BNIP3_HUMAN
NCBI Protein Information
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; OTTHUMP00000020752

NCBI Summary for BNIP3

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. This protein contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2. [provided by RefSeq]
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UniProt Comments for BNIP3

BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.

Protein type: Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytoplasm; dendrite; mitochondrial membrane; nuclear envelope; nucleus; integral to mitochondrial outer membrane

Molecular Function: identical protein binding; GTPase binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: cell death; positive regulation of protein complex disassembly; induction of apoptosis by granzyme; viral reproduction; positive regulation of apoptosis; apoptosis; positive regulation of programmed cell death; neuron apoptosis; response to hyperoxia; autophagic cell death; response to hypoxia; brown fat cell differentiation; regulation of mitochondrial membrane permeability; mitochondrial fragmentation during apoptosis; negative regulation of membrane potential; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis
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Research Articles on BNIP3

1. The high expression of HIF-1alpha and BNIP3 were closely related in laryngeal carcinomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BNIP3 antibody

 Reference Product  PubMed Publications
 BNIP3L antibody  >70 publications with BNIP3 and BNIP3L
 BCL2 antibody  >43 publications with BNIP3 and BCL2
 BCL2L1 antibody  >22 publications with BNIP3 and BCL2L1
 CD47 antibody  >3 publications with BNIP3 and CD47

Pathways associated with anti-BNIP3 antibody

 Products by Pathway  Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram

Diseases associated with anti-BNIP3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >135 publications with BNIP3 and Neoplasms
 Carcinoma Antibodies  >52 publications with BNIP3 and Carcinoma
 Cardiovascular Diseases Antibodies  >44 publications with BNIP3 and Cardiovascular Diseases
 Anoxia Antibodies  >40 publications with BNIP3 and Anoxia
 Adenocarcinoma Antibodies  >34 publications with BNIP3 and Adenocarcinoma
 Vascular Diseases Antibodies  >31 publications with BNIP3 and Vascular Diseases
 Colorectal Neoplasms Antibodies  >22 publications with BNIP3 and Colorectal Neoplasms
 Intestinal Diseases Antibodies  >20 publications with BNIP3 and Intestinal Diseases
 Breast Neoplasms Antibodies  >19 publications with BNIP3 and Breast Neoplasms
 Heart Failure Antibodies  >16 publications with BNIP3 and Heart Failure

Organs/Tissues associated with anti-BNIP3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >58 publications with BNIP3 and Muscle
 Heart Antibodies  >57 publications with BNIP3 and Heart
 Blood Antibodies  >36 publications with BNIP3 and Blood
 Brain Antibodies  >36 publications with BNIP3 and Brain
 Vascular Antibodies  >35 publications with BNIP3 and Vascular
 Liver Antibodies  >17 publications with BNIP3 and Liver
 Lung Antibodies  >15 publications with BNIP3 and Lung
 Bone Antibodies  >12 publications with BNIP3 and Bone
 Kidney Antibodies  >8 publications with BNIP3 and Kidney
 Prostate Antibodies  >8 publications with BNIP3 and Prostate
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