Rabbit Anti-MYL2 Polyclonal Antibody – Cat# MBS625852

Western blot analysis of extracts from HEK293 cells stimulated with ionophore A23187 for indicated times, using MBS625852:Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody.

Myosin Light Chain 2 (MYL2) Antibody – Rabbit Polyclonal

Name: MYL2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS625852
Price: 830 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625852

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth… More details

$830 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Myosin Light Chain 2 (MYL2), Polyclonal Antibody

Full Product Name

Myosin Light Chain 2, Phosphorylated (Thr18/Ser19)

Product Gene Name

anti-MYL2 antibody

Product Synonym Names

Anti -Myosin Light Chain 2, Phosphorylated (Thr18/Ser19)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

Purity/Purification

Affinity Purified
Purified by protein A and peptide affinity chromatography.

Specificity

Detects endogenous levels of Thr18 and Ser19 phosphorylated myosin light chain 2 (smooth muscle). Does not crossreact with the cardiac isoform of myosin light chain 2. Species Crossreactivity:

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

18,789 Da

Chromosome Location

Chromosome: 12; NC_000012.11 (111348623..111358404, complement). Location: 12q24.11

OMIM

160781

3D Structure

ModBase 3D Structure for P10916

Immunogen

Synthetic KLH-coupled phosphopeptide corresponding to residues surrounding Thr18/Ser19 of human myosin light chain 2. MW: 18kD

Product Categories/Family for anti-MYL2 antibody

Antibodies; Abs to Myosin

Applications Tested/Suitable for anti-MYL2 antibody

Western Blot (WB)

Application Notes for anti-MYL2 antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000

Other Notes

Small volumes of anti-MYL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYL2 purchase

MBS625852 is an antibody targeting MYL2. Also known as Myosin Light Chain 2, Phosphorylated (Thr18/Ser19). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MYL2. It may not necessarily be applicable to this product.

NCBI GI #

94981553

NCBI GeneID

4633

NCBI Accession #

NP_000423.2

UniProt Primary Accession #

P10916

UniProt Secondary Accession #

Q16123

UniProt Related Accession #

P10916; Q14908; Q6IB42; Q7Z3Z1

GenBank Nucleotide #

NM_000432.3

NCBI Official Full Name

myosin regulatory light chain 2, ventricular/cardiac muscle isoform

NCBI Official Synonym Full Names

myosin, light chain 2, regulatory, cardiac, slow

NCBI Official Symbol

MYL2

NCBI Official Synonym Symbols

MLC2; CMH10; DKFZp779C0562

UniProt Protein Name

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Protein Family

Myosin regulatory

UniProt Gene Name

MYL2

UniProt Entry Name

MLRV_HUMAN

NCBI Protein Information

myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow

NCBI Summary for MYL2

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq]

UniProt Comments for MYL2

MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: sarcomere; cytoskeleton; myofibril; cytosol; myosin complex; A band; actin cytoskeleton

Molecular Function: actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding

Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; muscle filament sliding; cardiac muscle contraction; post-embryonic development

Disease: Cardiomyopathy, Familial Hypertrophic, 10

Research Articles on MYL2

1. MYL2 was down-expressed in heart failure tissues, and findings suggested that MYL2 may play a role in the development and progression of chronic heart failure.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MYL2 antibody

Reference Product	PubMed Publications
USP6 antibody	>1 publications with MYL2 and USP6

Pathways associated with anti-MYL2 antibody

Products by Pathway	Pathway Diagram
CDC42 Signaling Events Pathway antibodies	CDC42 Signaling Events Pathway Diagram
Cardiac Muscle Contraction Pathway antibodies	Cardiac Muscle Contraction Pathway Diagram
Cardiac Muscle Contraction Pathway antibodies	Cardiac Muscle Contraction Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Hypertrophic Cardiomyopathy (HCM) Pathway antibodies	Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Hypertrophic Cardiomyopathy (HCM) Pathway antibodies	Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with anti-MYL2 antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>21 publications with MYL2 and Cardiovascular Diseases
Hypertrophy Antibodies	>8 publications with MYL2 and Hypertrophy
Hypertrophy, Left Ventricular Antibodies	>4 publications with MYL2 and Hypertrophy, Left Ventricular
Cardiomegaly Antibodies	>4 publications with MYL2 and Cardiomegaly
Metabolic Diseases Antibodies	>3 publications with MYL2 and Metabolic Diseases
Neoplasms Antibodies	>3 publications with MYL2 and Neoplasms
Heart Failure Antibodies	>3 publications with MYL2 and Heart Failure
CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 10 Antibodies	>3 publications with MYL2 and CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 10
Arrhythmias, Cardiac Antibodies	>2 publications with MYL2 and Arrhythmias, Cardiac
Obesity Antibodies	>2 publications with MYL2 and Obesity

Organs/Tissues associated with anti-MYL2 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>19 publications with MYL2 and Muscle
Heart Antibodies	>12 publications with MYL2 and Heart
Nerve Antibodies	>2 publications with MYL2 and Nerve
Embryonic Tissue Antibodies	>2 publications with MYL2 and Embryonic Tissue
Lymph Node Antibodies	>1 publications with MYL2 and Lymph Node
Bone Antibodies	>1 publications with MYL2 and Bone