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UBC9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626003

UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it… More details

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Product Overview

Online Datasheet in PDF
Product Name
UBC9, Polyclonal Antibody
Full Product Name
UBC9, NT (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9)
Product Gene Name
anti-UBC9 antibody
Product Synonym Names
Anti -UBC9, NT (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by ammonium sulfate precipitation.
Concentration
1.64mg/ml (lot specific)
Specificity
Recognizes human UBC9
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
17,911 Da
3D Structure
ModBase 3D Structure for P63279
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human UBC19, UniProt Accession #P63279.
Grade
Purified

Product Categories/Family for anti-UBC9 antibody

Antibodies; Abs to Ubiquitin Ligases
Applications Tested/Suitable for anti-UBC9 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-UBC9 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Western Blot: 1:1000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-UBC9 antibody

Western blot analysis of A2058 cell line lysates (35ug/lane) using MBS626003. UBE2I (arrow) was detected using the U0880-03.
Rabbit Anti-UBC9 Polyclonal Antibody – Cat# MBS626003

Immunohistochemistry (IHC) of anti-UBC9 antibody

Formalin-fixed and paraffin-embedded human cancer tissue using MBS626003, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-UBC9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS626003

Western Blot (WB) of anti-UBC9 antibody

Western blot analysis of 293 cell lysates (2ug/lane) using MBS626003 on either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2I gene.
Rabbit Anti-UBC9 Polyclonal Antibody, Western Blot (WB) – Cat# MBS626003

Other Notes

Small volumes of anti-UBC9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBC9 purchase

MBS626003 is an antibody targeting UBC9. Also known as UBC9, NT (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBC9. It may not necessarily be applicable to this product.

UniProt Primary Accession #
P63279
NCBI Official Full Name
UBC9
UniProt Protein Name
SUMO-conjugating enzyme UBC9
UniProt Synonym Protein Names
Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2-18 kDa; Ubiquitin-protein ligase
Protein Family
SUMO-conjugating enzyme
UniProt Gene Name
UBC9
UniProt Entry Name
UBC9_YEAST

UniProt Comments for UBC9

Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5

Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathway: Protein modification; protein sumoylation.

Subunit structure: Interacts with SIZ1. Ref.5 Ref.6

Subcellular location: Nucleus Ref.7.

Miscellaneous: Present with 2600 molecules/cell in log phase SD medium. Ref.8

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UBC9 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with UBC9 and FLNA

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