• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GNPAT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626080

GNPAT is an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GNPAT, Polyclonal Antibody
Full Product Name
GNPAT, CT (Glycerone-phosphate O-acyltransferase, Acyl-CoA:dihydroxyacetonephosphateacyltransferase, Dihydroxyacetone Phosphate Acyltransferase, DAPAT, DAP-AT, DHAPAT, DHAP-AT)
Product Gene Name
Product Synonym Names
Anti -GNPAT, CT (Glycerone-phosphate O-acyltransferase, Acyl-CoA:dihydroxyacetonephosphateacyltransferase, Dihydroxyacetone Phosphate Acyltransferase, DAPAT, DAP-AT, DHAPAT, DHAP-AT)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography
Specificity
Recognizes human GNPAT.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
77,664 Da
Chromosome Location
Chromosome: 28; AC_000185.1 (3378615..3418362, complement). Location: chromosome: 28
3D Structure
ModBase 3D Structure for A4IF87
Immunogen
Synthetic peptide corresponding to aa651-680 from the C-terminal region of human GNPAT conjugated to KLH.
Applications Tested/Suitable for anti-GNPAT antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-GNPAT antibody
Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry and Western Blot.
Dilution: ELISA: 1:1000
Immunohistochemistry: 1:50-1:100
Flow Cytometry:1:10-1:50
Western Blot: 1:100-1:500

Western Blot (WB) of anti-GNPAT antibody

Western blot analysis of GNPAT Antibody (C-term) in 293 cell line lysates (35ug/lane). GNPAT (arrow) was detected using the purified Pab.
Rabbit Anti-GNPAT Polyclonal Antibody – Cat# MBS626080

Immunohistochemistry (IHC) of anti-GNPAT antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with GNPAT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-GNPAT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS626080

Flow Cytometry (FC/FACS) of anti-GNPAT antibody

GNPAT Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-GNPAT Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS626080

Other Notes

Small volumes of anti-GNPAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GNPAT purchase

MBS626080 is an antibody targeting GNPAT. Also known as GNPAT, CT (Glycerone-phosphate O-acyltransferase, Acyl-CoA:dihydroxyacetonephosphateacyltransferase, Dihydroxyacetone Phosphate Acyltransferase, DAPAT, DAP-AT, DHAPAT, DHAP-AT). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GNPAT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
GNPAT protein
NCBI Official Symbol
GNPAT
UniProt Protein Name
Dihydroxyacetone phosphate acyltransferase
UniProt Synonym Protein Names
Acyl-CoA:dihydroxyacetonephosphateacyltransferase; Glycerone-phosphate O-acyltransferase
UniProt Gene Name
GNPAT
UniProt Synonym Gene Names
DAP-AT; DHAP-AT
UniProt Entry Name
GNPAT_BOVIN
NCBI Protein Information
dihydroxyacetone phosphate acyltransferase; DAP-AT; DHAP-AT; glycerone-phosphate O-acyltransferase; acyl-CoA:dihydroxyacetonephosphateacyltransferase

UniProt Comments for GNPAT

Catalytic activity: Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.

Pathway: Membrane lipid metabolism; glycerophospholipid metabolism.

Subunit structure: May be part of a heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT

By similarity.

Subcellular location: Peroxisome membrane; Peripheral membrane protein; Matrix side

By similarity. Note: Exclusively localized to the lumenal side of the peroxisomal membrane

By similarity.

Domain: The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate

By similarity.

Sequence similarities: Belongs to the GPAT/DAPAT family.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000