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CUL5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626128

CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to… More details

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Product Overview

Online Datasheet in PDF
Product Name
CUL5, Polyclonal Antibody
Full Product Name
CUL5, CT (VACM-1, Vasopressin-activated calcium-mobilizing receptor, Cullin-5, CUL-5, CUL5, VACM1)
Product Gene Name
anti-CUL5 antibody
Product Synonym Names
Anti -CUL5, CT (VACM-1, Vasopressin-activated calcium-mobilizing receptor, Cullin-5, CUL-5, CUL5, VACM1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human CUL5.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
90,955 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (107879408..107978488). Location: 11q22.3
OMIM
601741
3D Structure
ModBase 3D Structure for Q93034
Immunogen
Synthetic peptide selected from the C-terminal region of human CUL5 (KLH).

Product Categories/Family for anti-CUL5 antibody

Antibodies; Abs to Cullin
Applications Tested/Suitable for anti-CUL5 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-CUL5 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-CUL5 antibody

Western blot analysis of anti-CUL5 Antibody (C-term) in Hela cell line lysates (35ug/lane). CUL5 (arrow) was detected using the purified Pab.
Rabbit Anti-CUL5 Polyclonal Antibody – Cat# MBS626128

Flow Cytometry (FC/FACS) of anti-CUL5 antibody

Flow cytometric analysis of NCI-H292 cells using CUL5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CUL5 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS626128

Immunohistochemistry (IHC) of anti-CUL5 antibody

CUL5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CUL5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CUL5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS626128

Other Notes

Small volumes of anti-CUL5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CUL5 purchase

MBS626128 is an antibody targeting CUL5. Also known as CUL5, CT (VACM-1, Vasopressin-activated calcium-mobilizing receptor, Cullin-5, CUL-5, CUL5, VACM1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CUL5. It may not necessarily be applicable to this product.

NCBI GI #
40254446
NCBI GeneID
8065
NCBI Accession #
NP_003469.2
UniProt Primary Accession #
Q93034
UniProt Secondary Accession #
O14766; Q9BZC6; A8K960
UniProt Related Accession #
Q93034
GenBank Nucleotide #
NM_003478.3
NCBI Official Full Name
cullin-5
NCBI Official Synonym Full Names
cullin 5
NCBI Official Symbol
CUL5
NCBI Official Synonym Symbols
VACM1; VACM-1
UniProt Protein Name
Cullin-5
UniProt Synonym Protein Names
Vasopressin-activated calcium-mobilizing receptor 1
Protein Family
Cullin
UniProt Gene Name
CUL5
UniProt Synonym Gene Names
VACM1
UniProt Entry Name
CUL5_HUMAN
NCBI Protein Information
cullin-5; CUL-5; OTTHUMP00000232740; OTTHUMP00000232742; Vasopressin-activated calcium-mobilizing receptor-1; vasopressin-activated calcium-mobilizing receptor 1; Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1)

UniProt Comments for CUL5

CUL5: a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to be involved proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Member of the cullin family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: Cul5-RING ubiquitin ligase complex; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; protein heterodimerization activity; ubiquitin protein ligase binding; calcium channel activity; receptor activity

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of cell proliferation; cell proliferation; cytosolic calcium ion homeostasis; viral reproduction; protein ubiquitination; response to osmotic stress; cell cycle arrest; G1/S transition of mitotic cell cycle
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Research Articles on CUL5

1. Cul5 interacts with the Hsp90 chaperone complex and is recruited to the site of ErbB2 on the plasma membrane, thereby inducing its polyubiquitination and proteasome-mediated degradation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CUL5 antibody

 Reference Product  PubMed Publications
 RBX1 antibody  >18 publications with CUL5 and RBX1
 RNF7 antibody  >3 publications with CUL5 and RNF7

Pathways associated with anti-CUL5 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 Host Interactions Of HIV Factors Pathway antibodies  Host Interactions Of HIV Factors Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Vif-mediated Degradation Of APOBEC3G Pathway antibodies  Vif-mediated Degradation Of APOBEC3G Pathway Diagram

Diseases associated with anti-CUL5 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with CUL5 and Neoplasms
 Inflammation Antibodies  >6 publications with CUL5 and Inflammation
 Leukemia Antibodies  >5 publications with CUL5 and Leukemia
 Breast Neoplasms Antibodies  >3 publications with CUL5 and Breast Neoplasms
 Nervous System Diseases Antibodies  >3 publications with CUL5 and Nervous System Diseases
 Glioma Antibodies  >1 publications with CUL5 and Glioma
 Lung Neoplasms Antibodies  >1 publications with CUL5 and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >1 publications with CUL5 and Cell Transformation, Neoplastic
 Leukemia, Promyelocytic, Acute Antibodies  >1 publications with CUL5 and Leukemia, Promyelocytic, Acute
 Colonic Neoplasms Antibodies  >1 publications with CUL5 and Colonic Neoplasms

Organs/Tissues associated with anti-CUL5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >12 publications with CUL5 and Brain
 Blood Antibodies  >11 publications with CUL5 and Blood
 Vascular Antibodies  >10 publications with CUL5 and Vascular
 Kidney Antibodies  >8 publications with CUL5 and Kidney
 Liver Antibodies  >6 publications with CUL5 and Liver
 Lung Antibodies  >5 publications with CUL5 and Lung
 Heart Antibodies  >4 publications with CUL5 and Heart
 Muscle Antibodies  >3 publications with CUL5 and Muscle
 Nerve Antibodies  >3 publications with CUL5 and Nerve
 Ovary Antibodies  >2 publications with CUL5 and Ovary
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