Rabbit Anti-MEK1 Polyclonal Antibody – Cat# MBS626256

MEK1 Antibody – Rabbit Polyclonal

Name: MEK1 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS626256
Price: 510 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626256

MAP kinase kinase (Mek1), also known as mitogen activated protein kinase kinase, MAP kinase kinase, ERK kinase 1 or microtubule-associated protein 2 kinase, is a 43kD dual-specificity protein kinase that phosphorylates threonine and tyrosine residues on MAP… More details

$510 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MEK1, Polyclonal Antibody

Full Product Name

MEK1, phosphorylated (Ser217) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1)

Product Gene Name

anti-MEK1 antibody

Product Synonym Names

Anti -MEK1, phosphorylated (Ser217) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human MEK1 when phosphorylated at serine 217. Species Crossreactivity: mouse, rat.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

39,210 Da

Chromosome Location

Chromosome: 4; NC_003075.7 (13217649..13219933). Location: chromosome: 4

3D Structure

ModBase 3D Structure for Q94A06

Immunogen

Synthetic phosphopeptide corresponding to human MEK1 around the phosphorylation site of serine 217 (I-D-SP-M-A).

Product Categories/Family for anti-MEK1 antibody

Antibodies; Abs to Protein Kinases

Applications Tested/Suitable for anti-MEK1 antibody

Immunohistochemistry (IHC)

Application Notes for anti-MEK1 antibody

Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry: 1:50-1:100

Other Notes

Small volumes of anti-MEK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MEK1 purchase

MBS626256 is an antibody targeting MEK1. Also known as MEK1, phosphorylated (Ser217) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MEK1. It may not necessarily be applicable to this product.

NCBI GI #

2196704

NCBI GeneID

828713

NCBI Accession #

AAB97145.1

UniProt Primary Accession #

Q94A06

UniProt Secondary Accession #

O04440; O04661

UniProt Related Accession #

Q94A06

NCBI Official Full Name

MEK1

NCBI Official Symbol

MEK1

NCBI Official Synonym Symbols

ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK

UniProt Protein Name

Mitogen-activated protein kinase kinase 1

UniProt Synonym Protein Names

AtMEK1; NMAPKK

Protein Family

Meiosis-specific serine/threonine-protein kinase

UniProt Gene Name

MKK1

UniProt Synonym Gene Names

MEK1; MAP kinase kinase 1

UniProt Entry Name

M2K1_ARATH

NCBI Protein Information

mitogen-activated protein kinase kinase 1

NCBI Summary for MEK1

Member of MAP Kinase Kinase. Likely functions in a stress-activated MAPK pathway. Can phosphorylate the MAPK AtMPK4, in response to stress. Gets phosphorylated by MEKK1 in response to wounding.

UniProt Comments for MEK1

Function: May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MEK1/MKK2 and MPK4/MPK6). Phosphorylates MPK4 in vitro. Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated through serine and threonine phosphorylation in response to wounding, cold, drought, salt stresses, hydrogen peroxide, bacterial flagellin and laminarin beta-glucan. Ref.10 Ref.12

Subunit structure: Interacts with MEKK1 (MAPKKK) and MPK4. May form a ternary complex with. Ref.7

Tissue specificity: Expressed in roots, stem, flowers and siliques. Ref.1

Induction: By wounding. Ref.1 Ref.10 Ref.12

Post-translational modification: Phosphorylated on Thr-218 and Ser-224, which activates the enzyme. Ref.9 Ref.10

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.

Research Articles on MEK1

1. Data suggest that the MEKK1-MKK1/MKK2-MPK4 kinase cascade negatively regulates MEKK2 and activation of MEKK2 triggers SUMM2-mediated immune responses.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MEK1 antibody

Products by Pathway	Pathway Diagram
Plant-pathogen Interaction Pathway antibodies	Plant-pathogen Interaction Pathway Diagram
Plant-pathogen Interaction Pathway antibodies	Plant-pathogen Interaction Pathway Diagram

Organs/Tissues associated with anti-MEK1 antibody

Organ/Tissue Name	Pubmed Publications
Whole Plant Antibodies	>2 publications with MEK1 and Whole Plant