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Vesicular Monoamine Transporter 1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626333

MBS626333 is an antibody targeting Vesicular Monoamine Transporter 1. Also known as Anti-Vesicular Monoamine Transporter 1 (VMAT1). Reactive in Rat. Host: Rabbit. More details

$980 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Vesicular Monoamine Transporter 1, Polyclonal Antibody
Full Product Name
Anti-Vesicular Monoamine Transporter 1 (VMAT1)
Product Synonym Names
VMAT1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Purity/Purification
Affinity Purified
Concentration
1mg/ml (lot specific)
Specificity
Recognizes rat VMAT1. Does not recognize VMAT2 in transfected cells (1, 2). The C-terminal rat VMAT1 sequence does not show any appreciable homology with hVMAT1 (4). Antibodies to rVMAT1 are not expected to crossreact with hVMAT1.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Immunogen
A 12 AA peptide sequence near the C-terminus of rat VMAT1 (1). This peptide is predicted to be cytoplasmic. The peptide was synthesized with amino terminal Cysteine, coupled to KLH.
Source Information
Rat
Applications Tested/Suitable for anti-Vesicular Monoamine Transporter 1 antibody
ELISA; Western Blot
Recommended Dilutions: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.

Other Notes

Small volumes of anti-Vesicular Monoamine Transporter 1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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