Rabbit Anti-PRKD2 Polyclonal Antibody – Cat# MBS626404

Unstimlated (lane 1) and PDBu stimulated (lane 2) HEK293 cell lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with antiphospho-PKD2 (Ser876) (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKD2 (Ser876) (105kD).

Protein Kinase D2 (PRKD2) Antibody – Rabbit Polyclonal

Name: PRKD2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS626404
Price: 715 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626404

Protein kinase D2 (PKD2) is a novel human serine threonine protein kinase gene. It exhibits the strongest homology to the serine threonine protein kinases PKD/PKC; and PKCnu, particularly in the duplex zinc nger-like cysteine-rich motif, in the pleckstrin… More details

$715 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Protein Kinase D2 (PRKD2), Polyclonal Antibody

Full Product Name

Protein Kinase D2, phosphorylated (Ser876) (PKD2)

Product Gene Name

anti-PRKD2 antibody

Product Synonym Names

Anti -Protein Kinase D2, phosphorylated (Ser876) (PKD2)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human phospho-PKD2 (Ser876), Mr ~105kD. Two nonspecific proteins were also detected, one at ~120 kD and another at ~50 kD. The nonspecific bands were not competed by the immunizing peptide, and thus can serve as a loading control.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

96,750 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (47177573..47220384, complement). Location: 19q13.3

OMIM

607074

3D Structure

ModBase 3D Structure for Q9BZL6

Immunogen

KLH-conjugated, synthetic peptide corresponding to aa surrounding phosphorylated Ser876 of human PKD2.

Product Categories/Family for anti-PRKD2 antibody

Antibodies; Abs to Protein Kinases

Applications Tested/Suitable for anti-PRKD2 antibody

Western Blot (WB)

Application Notes for anti-PRKD2 antibody

Suitable for use in Western Blot.
Dilution: Immunoblot Analysis: 0.5-2ug/ml of this lot detected phospho-PKD2 (Ser876) in RIPA lysates from PDBu (Phorbol 12,13-dibutyrate) stimulated HEK293 cells.

Other Notes

Small volumes of anti-PRKD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRKD2 purchase

MBS626404 is an antibody targeting PRKD2. Also known as Protein Kinase D2, phosphorylated (Ser876) (PKD2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKD2. It may not necessarily be applicable to this product.

NCBI GI #

12659007

NCBI GeneID

25865

UniProt Primary Accession #

Q9BZL6

UniProt Secondary Accession #

Q8TB08; Q9P0T6; Q9Y3X8

UniProt Related Accession #

Q8N2H2; Q8NCK8; Q9BZL6

NCBI Official Full Name

protein kinase D2

NCBI Official Synonym Full Names

protein kinase D2

NCBI Official Symbol

PRKD2

NCBI Official Synonym Symbols

PKD2; HSPC187; nPKC-D2

UniProt Protein Name

Serine/threonine-protein kinase D2

UniProt Synonym Protein Names

nPKC-D2

Protein Family

Serine/threonine-protein kinase

UniProt Gene Name

PRKD2

UniProt Synonym Gene Names

PKD2

UniProt Entry Name

KPCD2_HUMAN

NCBI Protein Information

serine/threonine-protein kinase D2

NCBI Summary for PRKD2

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

UniProt Comments for PRKD2

PRKD2: a calcium-independent, phospholipid-dependent CAMK kinase of the PKD family. Activated by diacylglycerol and phorbol esters. may play a role in carcinoid tumor progression.

Protein type: EC 2.7.11.13; Motility/polarity/chemotaxis; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); CAMK group; PKD family

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: cell death; positive regulation of cell adhesion; protein amino acid autophosphorylation; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of fibroblast growth factor receptor signaling pathway; T cell receptor signaling pathway; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; peptidyl-serine phosphorylation; positive regulation of angiogenesis; positive regulation of T cell receptor signaling pathway; positive regulation of interleukin-2 production; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; angiogenesis; cell adhesion; vascular endothelial growth factor receptor signaling pathway

Research Articles on PRKD2

1. Expression and kinase activity of PKD2 are required for the ligand-inducible stimulation of IFNAR1 ubiquitination and endocytosis and for accelerated proteolytic turnover of IFNAR1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PRKD2 antibody

Products by Pathway	Pathway Diagram
T Cell Receptor Signaling Pathway antibodies	T Cell Receptor Signaling Pathway Diagram

Diseases associated with anti-PRKD2 antibody

Disease Name	Pubmed Publications
Leukemia, Lymphocytic, Chronic, B-Cell Antibodies	>1 publications with PRKD2 and Leukemia, Lymphocytic, Chronic, B-Cell

Organs/Tissues associated with anti-PRKD2 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1 publications with PRKD2 and Blood