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ERP29 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626505

ERP29 shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to… More details

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Product Overview

Online Datasheet in PDF
Product Name
ERP29, Polyclonal Antibody
Full Product Name
ERP29, ID (Endoplasmic Reticulum Resident Protein 29, Endoplasmic Reticulum Resident Protein 31, Endoplasmic Reticulum Resident Protein 28, ERp31, ERp28, C12orf8)
Product Gene Name
Product Synonym Names
Anti -ERP29, ID (Endoplasmic Reticulum Resident Protein 29, Endoplasmic Reticulum Resident Protein 31, Endoplasmic Reticulum Resident Protein 28, ERp31, ERp28, C12orf8)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ERP29.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
28,993 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (112451152..112461024). Location: 12q24.13
OMIM
602287
3D Structure
ModBase 3D Structure for P30040
Immunogen
Synthetic peptide selected from the Center region of human ERP29 (KLH).
Applications Tested/Suitable for anti-ERP29 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-ERP29 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-ERP29 antibody

Western blot analysis using MBS626505 and cell line lysates (35ug/lane) Lane 1: A2058 Lane 2: A375 Lane 3: MCF7 Lane 4: NCI-H460 Lane 5: Y79 The ERP29 protein(arrow) was detected .
Rabbit Anti-ERP29 Polyclonal Antibody – Cat# MBS626505

Western Blot (WB) of anti-ERP29 antibody

Western Blot analysis in 293 cell lysates (2ug/lane) using MBS626505 either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2)
Rabbit Anti-ERP29 Polyclonal Antibody, Western Blot (WB) – Cat# MBS626505

Immunohistochemistry (IHC) of anti-ERP29 antibody

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue using MBS626505 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS626505 for immunohistochemistry.
Rabbit Anti-ERP29 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS626505

Flow Cytometry (FC/FACS) of anti-ERP29 antibody

Flow Cytometric analysis of NCI-H292 cells using MBS626505 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ERP29 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS626505

Other Notes

Small volumes of anti-ERP29 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ERP29 purchase

MBS626505 is an antibody targeting ERP29. Also known as ERP29, ID (Endoplasmic Reticulum Resident Protein 29, Endoplasmic Reticulum Resident Protein 31, Endoplasmic Reticulum Resident Protein 28, ERp31, ERp28, C12orf8). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ERP29. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
endoplasmic reticulum resident protein 29 isoform 1
NCBI Official Synonym Full Names
endoplasmic reticulum protein 29
NCBI Official Symbol
ERP29
NCBI Official Synonym Symbols
ERp28; ERp31; PDIA9; PDI-DB; C12orf8
UniProt Protein Name
Endoplasmic reticulum resident protein 29
UniProt Synonym Protein Names
Endoplasmic reticulum resident protein 28; ERp28; Endoplasmic reticulum resident protein 31
UniProt Gene Name
ERP29
UniProt Synonym Gene Names
C12orf8; ERP28
UniProt Entry Name
ERP29_HUMAN
NCBI Protein Information
endoplasmic reticulum resident protein 29; endoplasmic reticulum resident protein 28; endoplasmic reticulum resident protein 31; endoplasmic reticulum lumenal protein ERp28; protein disulfide isomerase family A, member 9

NCBI Summary for ERP29

This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
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UniProt Comments for ERP29

ERP29: Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: transport vesicle; cell surface; smooth endoplasmic reticulum; membrane; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome

Molecular Function: protein binding; protein homodimerization activity; chaperone binding; protein disulfide isomerase activity

Biological Process: intracellular protein transport; negative regulation of protein secretion; protein folding; activation of MAPK activity; protein unfolding; protein secretion; positive regulation of protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ERP29 antibody

 Reference Product  PubMed Publications
 HSPA5 antibody  >1 publications with ERP29 and HSPA5
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