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Phosphodiesterase 4d (PDE4D) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626579

This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses… More details

$835 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Phosphodiesterase 4D (PDE4D), Polyclonal Antibody
Full Product Name
Phosphodiesterase 4D (Phosphodiesterase 4D cAMP Specific, Phosphodiesterase-4D, PDE4D, cAMP Specific 3' 5' Cyclic Phosphodiesterase 4D, cAMP Specific Phosphodiesterase 4D, cAMP Specific Phosphodiesterase PDE4D, Cyclic AMP Phosphodiesterase, Dunce-like Pho
Product Gene Name
Product Synonym Names
Anti -Phosphodiesterase 4D (Phosphodiesterase 4D cAMP Specific, Phosphodiesterase-4D, PDE4D, cAMP Specific 3' 5' Cyclic Phosphodiesterase 4D, cAMP Specific Phosphodiesterase 4D, cAMP Specific Phosphodiesterase PDE4D, Cyclic AMP Phosphodiesterase, Dunce-like Pho
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Human
Form/Format
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by peptide affinity chromatography.
Specificity
Recognizes human Phosphodiesterase 4D. Not expected to recognize isoform 4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 21; NC_009164.2 (12541854..13872904). Location: chromosome: 21
Immunogen
Synthetic peptide corresponding to QPEACVIDDRSPDT, from the C Terminus of human Phosphodiesterase 4D (NP_001098101.1; NP_006194.2; NP_001159371.1).
Applications Tested/Suitable for anti-PDE4D antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-PDE4D antibody
Suitable for use in ELISA and Western Blot.
Dilution: Peptide ELISA Titer: 1:128,000
Western Blot: 0.1-0.3ug/ml ~70+80+90kD bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of ~68kD and 95kD are observed. These bands are not observed in the untransfected COS1. In transfected HEK293 transiently expressing PDE4D2 multi bands from ~55kD-80kD are observed. These bands are not observed in the non-transfected HEK293. Calculated MW of 91.1kD according to PDE4D1 (NP_001098101.1), 76.5kD according to PDE4D2 (NP_006194.2) and 84.7kD according to PDE4D3 (NP_001159371.1).

Chemiluminescence of anti-PDE4D antibody

MBS626579 Staining (1µg/ml) of COS cell lysates (25µg protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PDE4D Polyclonal Antibody – Cat# MBS626579

Testing Data of anti-PDE4D antibody

HEK293 overexpressing Human PDE4D2 and probed with (mock transfection in first lane), tested by Origene.
Goat Anti-PDE4D Polyclonal Antibody, Testing Data – Cat# MBS626579

Other Notes

Small volumes of anti-PDE4D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDE4D purchase

MBS626579 is an antibody targeting PDE4D. Also known as Phosphodiesterase 4D (Phosphodiesterase 4D cAMP Specific, Phosphodiesterase-4D, PDE4D, cAMP Specific 3' 5' Cyclic Phosphodiesterase 4D, cAMP Specific Phosphodiesterase 4D, cAMP Specific Phosphodiesterase PDE4D, Cyclic AMP Phosphodiesterase, Dunce-like Pho. Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE4D. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Official Full Name
phosphodiesterase 4D
NCBI Official Symbol
PDE4D
NCBI Protein Information
phosphodiesterase 4D, cAMP-specific; phosphodiesterase E3 dunce homolog, Drosophila

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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