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Myod Family Inhibitor (MDFI) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626690

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding.… More details

$835 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MDFI MyoD Family Inhibitor, Polyclonal Antibody
Full Product Name
MDFI MyoD Family Inhibitor (I-MF, I-mfa, Myogenic Repressor I-mf)
Product Gene Name
anti-MDFI antibody
Product Synonym Names
Anti -MDFI MyoD Family Inhibitor (I-MF, I-mfa, Myogenic Repressor I-mf)
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Human
Form/Format
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by peptide affinity chromatography.
Specificity
Recognizes MDFI. Species sequence homology: human.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
25,029 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (41606195..41621982). Location: 6p21
OMIM
604971
3D Structure
ModBase 3D Structure for Q99750
Immunogen
Synthetic peptide corresponding to C-SKSTTSQIPLQAQED, from the internal region of human MDFI (NP_005577.1).

Product Categories/Family for anti-MDFI antibody

Antibodies; Abs to Cancer Markers
Applications Tested/Suitable for anti-MDFI antibody
ELISA (EL/EIA)
Application Notes for anti-MDFI antibody
Suitable for use in ELISA.
Dilution: Peptide ELISA Titer: 1:16,000

Other Notes

Small volumes of anti-MDFI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MDFI purchase

MBS626690 is an antibody targeting MDFI. Also known as MDFI MyoD Family Inhibitor (I-MF, I-mfa, Myogenic Repressor I-mf). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for MDFI. It may not necessarily be applicable to this product.

NCBI GI #
5031905
NCBI GeneID
4188
NCBI Accession #
NP_005577.1
UniProt Primary Accession #
Q99750
UniProt Related Accession #
Q99750
GenBank Nucleotide #
NM_005586.3
NCBI Official Full Name
myoD family inhibitor
NCBI Official Synonym Full Names
MyoD family inhibitor
NCBI Official Symbol
MDFI
NCBI Official Synonym Symbols
I-MF; I-mfa
UniProt Protein Name
MyoD family inhibitor
UniProt Synonym Protein Names
Myogenic repressor I-mf
Protein Family
MyoD family inhibitor
UniProt Gene Name
MDFI
UniProt Entry Name
MDFI_HUMAN
NCBI Protein Information
myoD family inhibitor; OTTHUMP00000016375; OTTHUMP00000016376; OTTHUMP00000196103; OTTHUMP00000196414; OTTHUMP00000196415; myogenic repressor I-mf; inhibitor of MyoD family a

UniProt Comments for MDFI

MDFI: Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. Belongs to the MDFI family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; transcription factor binding

Biological Process: embryonic development; cytoplasmic sequestering of transcription factor; negative regulation of Wnt receptor signaling pathway; dorsal/ventral axis specification; negative regulation of transcription from RNA polymerase II promoter; activation of JNK activity; negative regulation of DNA binding; embryonic skeletal morphogenesis
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Research Articles on MDFI

1. I-mfa functions as a molecular switch to suppress the store dependence of TRPC1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MDFI antibody

 Reference Product  PubMed Publications
 TRPC1 antibody  >1 publications with MDFI and TRPC1
 TCF3 antibody  >1 publications with MDFI and TCF3
 MYOG antibody  >1 publications with MDFI and MYOG

Diseases associated with anti-MDFI antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >2 publications with MDFI and Carcinoma
 Adenocarcinoma Antibodies  >1 publications with MDFI and Adenocarcinoma
 Neoplasms Antibodies  >1 publications with MDFI and Neoplasms
 Pancreatic Neoplasms Antibodies  >1 publications with MDFI and Pancreatic Neoplasms

Organs/Tissues associated with anti-MDFI antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >6 publications with MDFI and Muscle
 Placenta Antibodies  >3 publications with MDFI and Placenta
 Embryonic Tissue Antibodies  >3 publications with MDFI and Embryonic Tissue
 Bone Antibodies  >2 publications with MDFI and Bone
 Kidney Antibodies  >2 publications with MDFI and Kidney
 Blood Antibodies  >2 publications with MDFI and Blood
 Brain Antibodies  >2 publications with MDFI and Brain
 Liver Antibodies  >2 publications with MDFI and Liver
 Skin Antibodies  >1 publications with MDFI and Skin
 Testis Antibodies  >1 publications with MDFI and Testis
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