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XRCC1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6267068

MBS6267068 is an antibody targeting XRCC1. Also known as XRCC1 (X-Ray Repair Cross Complementing Protein1) (BSA & Azide Free) (PE). Reactive in Human, Rat. Host: Mouse. More details

$1305 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
XRCC1, Monoclonal Antibody
Full Product Name
XRCC1 (X-Ray Repair Cross Complementing Protein1) (BSA & Azide Free) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3F399
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes XRCC1 (X-Ray Repair Cross Complementing Protein1). Species Crossreactivity: human and rat.
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Immunogen
Recombinant human XRCC1 protein
Conjugate
PE
Applications Tested/Suitable for anti-XRCC1 antibody
Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

Testing Data of anti-XRCC1 antibody

Mouse Anti-XRCC1 Monoclonal Antibody – Cat# MBS6267068

Immunohistochemistry (IHC) of anti-XRCC1 antibody

Formalin-fixed, paraffin-embedded rat testis stained with XRCC1 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.
Mouse Anti-XRCC1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6267068

Product References and Citations for anti-XRCC1 antibody

1. Cappelli E; et al. J Bio Chem, 1997, 272:23970-5. 2. Labudova O; et al. Pediatric Res, 1997, 41:435-9. Additional: 1. Nash RA; Caldecott KW; Barnes DE; Lindahl T. XRCC1 protein interacts with one of two distinct forms of DNA ligase III. Biochemistry, 1997, 36(17):5207-11. 2. Andersson BS; Sadeghi T; Siciliano MJ; Legerski R; Murray D. Nucleotide excision repair genes as determinants of cellular sensitivity to cyclophosphamide analogs. Cancer Chemotherapy and Pharmacology, 1996, 38(5):406-16. 3. Caldecott KW; Aoufouchi S; Johnson P; Shall S. XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro. Nucleic Acids Research, 1996, 24:4387-94. 4. Kubota Y; Nash RA; Klungland A; Schar P; Barnes DE; Lindahl T. Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein. Embo Journal, 1996, 15:6662-70. 5. Walter CA; Trolian DA; McFarland MB; Street KA; Gurram GR; McCarrey JR. Xrcc-1 expression during male meiosis in the mouse. Biology of Reproduction, 1996 Sep, 55(3):630- 5. 6. Caldecott KW; Tucker JD; Stanker LH; Thompson LH. Characterization of the XRCC1- DNA ligase III complex in vitro and its absence from mutant hamster cells. Nucleic Acids Research, 1995, 23(23):4836-43. 7. Lamerdin JE; Montgomery MA; Stilwagen SA; Scheidecker LK; Tebbs RS; Brookman KW; Thompson LH; Carrano AV. Genomic sequence comparison of the human and mouse XRCC1 DNA repair gene regions. Genomics, 1995, 25(2):547-54. 8. Wei YF; Robins P; Carter K; Caldecott K; Pappin DJ; Yu GL; Wang RP; Shell BK; Nash RA; Schar P; et al. Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination. Molecular and Cellular Biology, 1995, 15(6):3206-16. 9. Zhou ZQ; Walter CA. Expression of the DNA repair gene XRCC1 in baboon tissues. Mutation Research, 1995 Nov, 348(3):111-6. 10. Brookman KW; Tebbs RS; Allen SA; Tucker JD; Swiger RR; Lamerdin JE; Carrano AV; Thompson LH. Isolation and characterization of mouse Xrcc-1, a DNA repair gene affecting ligation. Genomics, 1994 Jul 1, 22(1):180-8. 11. Caldecott KW; McKeown CK; Tucker JD; Ljungquist S; Thompson LH. An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III. Molecular and Cellular Biology, 1994, 14(1):68-76. 12. Caldecott KW; Thompson LH. Partial correction of the single-strand break repair defect in the CHO mutant EM9 by electroporated recombinant XRCC1 protein. Annals of the New York Academy of Sciences, 1994 Jul 29, 726:336-9. 13. Ljungquist S; Kenne K; Olsson L; Sandstrom M. Altered DNA ligase III activity in the CHO EM9 mutant. Mutation Research, 1994, 314(2):177- 86. 14. Shung B; Miyakoshi J; Takebe H. X-rayinduced transcriptional activation of c-myc and XRCC1 genes in ataxia telangiectasia cells. Mutation Research, 1994, 307(1):43-51. 15. Walter CA; Lu J; Bhakta M; Zhou ZQ; Thompson LH; McCarrey JR. Testis and somatic Xrcc-1 DNA repair gene expression. Somatic Cell and Molecular Genetics, 1994, 20(6):451-61. 16. Lehmann AR. Duplicated region of sequence similarity to the human XRCC1 DNA repair gene in the Schizosaccharomyces pombe rad4/cut5 gene. Nucleic Acids Research, 1993, 21(22):5274

Other Notes

Small volumes of anti-XRCC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for XRCC1. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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