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Ribosomal Protein S6 (Despr_1827) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626735

40S ribosomal protein S6 (also known as RPS6) is a ~31kD substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. Phosphorylation of RPS6 is rapamycin and… More details

$980 / 10 Blots +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ribosomal Protein S6 (Despr_1827), Polyclonal Antibody
Full Product Name
Ribosomal Protein S6, phosphorylated (Ser244, Ser247)
Product Gene Name
Product Synonym Names
Anti -Ribosomal Protein S6, phosphorylated (Ser244, Ser247)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS pH 7.3,1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Specificity
Recognizes human RPS6. Species Sequence Homology
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chromosome Location
Chromosome: ANONYMOUS; NC_014972.1 (2098706..2099131)
Immunogen
Synthetic phosphopeptide derived from the region of human RPS6 that contains serines 244 and 247. The sequence is conserved in mouse and rat.
Applications Tested/Suitable for anti-Despr_1827 antibody
Western Blot (WB)
Application Notes for anti-Despr_1827 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000 dilution.

SDS-Page of anti-Despr_1827 antibody

Up-regulation and Antibody-Peptide Competition. Peptide Competition. Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for 1 hour at RT, incubated with MBS626735 antibody for 2 hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1,2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to MBS626735 blocks the signal, verifying the specificity of the antibody.
Rabbit Anti-Despr_1827 Polyclonal Antibody – Cat# MBS626735

Other Notes

Small volumes of anti-Despr_1827 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Despr_1827 purchase

MBS626735 is an antibody targeting Despr_1827. Also known as Ribosomal Protein S6, phosphorylated (Ser244, Ser247). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Despr_1827. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Official Full Name
ribosomal protein S6
NCBI Official Symbol
Despr_1827
NCBI Protein Information
ribosomal protein S6

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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