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Actin, Alpha, Smooth Muscle Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6267756

MBS6267756 is an antibody targeting Actin, alpha, Smooth Muscle. Also known as Actin, alpha, Smooth Muscle (BSA, Glycerol & Azide Free) (MaxLight 650). Reactive in Bovine, Chicken, Human, Mouse, Rabbit, Rat. Host: Mouse. More details

$920 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Actin, alpha, Smooth Muscle, Monoclonal Antibody
Full Product Name
Actin, alpha, Smooth Muscle (BSA, Glycerol & Azide Free) (MaxLight 650)
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
0.N.5
Host
Mouse
Species Reactivity
Bovine, Chicken, Human, Mouse, Rabbit, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Purity/Purification
Purified by Protein A Affinity Chromatography
Specificity
Recognizes the alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells. Does not crossreact with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). Species Crossreactivity: Human, baboon, bovine, rabbit, mouse, rat and chicken.
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Immunogen
N-terminal decapeptide of the alpha smooth muscle isoform of actin; acetylated at the N-terminus. Epitope Location: Acetyl group and the first 4 amino acids on the N-terminal end of the peptidic chain of alpha-smooth actin. Cellular Localization: Cytoplasmic.
Conjugate
MaxLight650
Applications Tested/Suitable for anti-Actin, alpha, Smooth Muscle antibody
Immunofluorescence (IF), Immunohistochemistry (IHC)

Immunohistochemistry (IHC) of anti-Actin, alpha, Smooth Muscle antibody

Immunohistochemistry of formalin-fixed, paraffin-embedded human leiomyoma using MBS633240 followed by peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.
Mouse Anti-Actin, Alpha, Smooth Muscle Monoclonal Antibody – Cat# MBS6267756

Product References and Citations for anti-Actin, alpha, Smooth Muscle antibody

1. Bochaton-Piallat ML; Gabbiani F; Gabbiani G. Heterogeneity of rat aortic smooth muscle cell replication during development: correlation with replicative activity after experimental endothelial denudation in adults. Journal of Submicroscopic Cytology and Pathology, 1994, 26(1):1-8. 2. Cremona O; Savoia P; Marchisio PC; Gabbiani G; Chaponnier C. The alpha 6 and beta 4 integrin subunits are expressed by smooth muscle cells of human small vessels: a new localization in mesenchymal cells. Journal of Histochemistry and Cytochemistry, 1994, 42(9):1221-8. 3. Estes JM; Vande Berg JS; Adzick NS; MacGillivray TE; Desmouliere A; Gabbiani G. Phenotypic and functional features of myofibroblasts in sheep fetal wounds. Differentiation, 1994, 56(3):173-81. 4. Orlandi A; Ehrlich HP; Ropraz P; Spagnoli LG; Gabbiani G. Rat aortic smooth muscle cells isolated from different layers and at different times after endothelial denudation show distinct biological features in vitro. Arteriosclerosis and Thrombosis, 1994, 14(6):982-9.

Other Notes

Small volumes of anti-Actin, alpha, Smooth Muscle antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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