Mouse Anti-GZMA Monoclonal Antibody – Cat# MBS6268813

Granzyme A (GZMA) Antibody – Mouse Monoclonal

Name: GZMA Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6268813
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6268813

MBS6268813 is an antibody targeting GZMA. Also known as Granzyme A (APC). Reactive in Human. Host: Mouse. More details

$1060 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Granzyme A (GZMA), Monoclonal Antibody

Full Product Name

Granzyme A (APC)

Product Gene Name

anti-GZMA antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

3H2176

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Purity/Purification

Purified by protein A affinity chromatography from tissue culture supernatant.

Specificity

Recognizes human Granzyme A, a ~60kD disulfide-linked homodimeric protein of two 262aa chains, expressed in cytoplasmic granules of cytotoxic lymphocytes and NK cells.

Preparation and Storage

Store at 4 degree C. Do Not Freeze.

Molecular Weight

28,999 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (54398474..54406080). Location: 5q11-q12

OMIM

140050

3D Structure

ModBase 3D Structure for P12544

Immunogen

Recombinant protein corresponding to human Granzyme A.

Conjugate

APC

Product Categories/Family for anti-GZMA antibody

Antibodies; Apoptosis

Applications Tested/Suitable for anti-GZMA antibody

Immunohistochemistry (IHC), Western Blot (WB)

Product References and Citations for anti-GZMA antibody

1. Kummer, J. A., et al. Production and characterisation of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins. J. Immunol. Methods. 163: 73-83 (1993). 2. Mahrus, S. & Craik, C.S. (2005) Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem Biol. 12: 567-77. 3. Meade, J.L. et al. (2009) Proteolytic activation of the cytotoxic phenotype during human NK cell development. J Immunol. 183: 803-13. 4. Hochegger, K. et al. (2007) Expression of granzyme A in human polymorphonuclear neutrophils. Immunology. 121: 166-73. 5. Grodzovski, I. et al. (2011) IL-2-granzyme A chimeric protein overcomes multidrug resistance (MDR) through a caspase 3-independent apoptotic pathway. Int J Cancer. 128: 1966-80. 6. Suck, G. et al. (2005) KHYG-1, a model for the study of enhanced natural killer cell cytotoxicity. Exp Hematol. 33: 1160-71. 7. Vrazo, A.C. et al. (2015) Live cell evaluation of granzyme delivery and death receptor signaling in tumor cells targeted by human natural killer cells. Blood. 126 (8): e1-e10. 8. Korten, S. et al. (2008) Natural death of adult Onchocerca volvulus and filaricidal effects of doxycycline induce local FOXP3+/CD4+ regulatory T cells and granzyme expression. Microbes Infect. 10 (3): 313-24.

Other Notes

Small volumes of anti-GZMA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GZMA. It may not necessarily be applicable to this product.

NCBI GI #

5453676

NCBI GeneID

3001

NCBI Accession #

NP_006135.1

UniProt Primary Accession #

P12544

UniProt Secondary Accession #

Q6IB36; A4PHN1

UniProt Related Accession #

P12544

GenBank Nucleotide #

NM_006144.3

NCBI Official Full Name

granzyme A

NCBI Official Synonym Full Names

granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)

NCBI Official Symbol

GZMA

NCBI Official Synonym Symbols

HFSP; CTLA3

UniProt Protein Name

Granzyme A

UniProt Synonym Protein Names

CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor

Protein Family

Granzyme

UniProt Gene Name

GZMA

UniProt Synonym Gene Names

CTLA3; HFSP; H factor; HF

UniProt Entry Name

GRAA_HUMAN

NCBI Protein Information

granzyme A; HF; h factor; CTL tryptase; fragmentin-1; cytotoxic T-lymphocyte proteinase 1; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease)

NCBI Summary for GZMA

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]

UniProt Comments for GZMA

GZMA: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. Homodimer; disulfide-linked. Interacts with APEX1. Dexamethasone (DEX) induces expression of isoform beta and represses expression of isoform alpha. The alteration in expression is mediated by binding of glucocorticoid receptor to independent promoters adjacent to the alternative first exons of isoform alpha and isoform beta. Belongs to the peptidase S1 family. Granzyme subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Apoptosis; Secreted, signal peptide; Protease; EC 3.4.21.78; Secreted

Chromosomal Location of Human Ortholog: 5q11-q12

Cellular Component: extracellular region; immunological synapse; nucleus

Molecular Function: protein binding; protein homodimerization activity; serine-type endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; positive regulation of apoptosis; apoptosis; cytolysis; negative regulation of oxidoreductase activity; negative regulation of endodeoxyribonuclease activity; immune response; negative regulation of DNA binding

Research Articles on GZMA

1. Patients with chronic immune thrombocytopenia have elevated serum granzyme A levels.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GZMA antibody

Reference Product	PubMed Publications
PRF1 antibody	>140 publications with GZMA and PRF1
GZMK antibody	>11 publications with GZMA and GZMK
GNLY antibody	>6 publications with GZMA and GNLY
IL2 antibody	>2 publications with GZMA and IL2

Pathways associated with anti-GZMA antibody

Products by Pathway	Pathway Diagram
IL12-mediated Signaling Events Pathway antibodies	IL12-mediated Signaling Events Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with anti-GZMA antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>32 publications with GZMA and Necrosis
Inflammation Antibodies	>15 publications with GZMA and Inflammation
Disease Models, Animal Antibodies	>7 publications with GZMA and Disease Models, Animal
Drug Toxicity Antibodies	>5 publications with GZMA and Drug Toxicity
Hypersensitivity Antibodies	>5 publications with GZMA and Hypersensitivity
Gastrointestinal Diseases Antibodies	>5 publications with GZMA and Gastrointestinal Diseases
Lung Diseases Antibodies	>4 publications with GZMA and Lung Diseases
Blood Coagulation Disorders Antibodies	>3 publications with GZMA and Blood Coagulation Disorders
Liver Diseases Antibodies	>3 publications with GZMA and Liver Diseases
Kidney Diseases Antibodies	>2 publications with GZMA and Kidney Diseases

Organs/Tissues associated with anti-GZMA antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>261 publications with GZMA and Blood
Lung Antibodies	>10 publications with GZMA and Lung
Skin Antibodies	>10 publications with GZMA and Skin
Spleen Antibodies	>9 publications with GZMA and Spleen
Kidney Antibodies	>9 publications with GZMA and Kidney
Heart Antibodies	>8 publications with GZMA and Heart
Intestine Antibodies	>8 publications with GZMA and Intestine
Uterus Antibodies	>5 publications with GZMA and Uterus
Lymph Node Antibodies	>5 publications with GZMA and Lymph Node
Brain Antibodies	>3 publications with GZMA and Brain