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His-Tag Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6268840

MBS6268840 is an antibody targeting His-Tag. Also known as His-Tag (6-Histidine, 6 His, 6-His Epitope Tag, HHHHHH Epitope Tag, HHHHHH Tag, Polyhistidine Tag) (APC). Host: Mouse. More details

$875 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
His-Tag, Monoclonal Antibody
Full Product Name
His-Tag (6-Histidine, 6 His, 6-His Epitope Tag, HHHHHH Epitope Tag, HHHHHH Tag, Polyhistidine Tag) (APC)
Product Synonym Names
Histidine Tag
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10D149
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Recognizes all His tagged proteins so far tested. Has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian cell lines. In Western Blot of bacterial extracts, does not crossreact with any endogenous products, though some crossreactivity may be seen with extracts of insect or mammalian cells.
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Immunogen
PAX6 transcription factor linked to 6-histidine tag (HHHHHH).
Conjugate
APC

Product Categories/Family for anti-His-Tag antibody

Applications Tested/Suitable for anti-His-Tag antibody
FLISA, Immunoprecipitation (IP), Western Blot (WB)

Testing Data of anti-His-Tag antibody

His-ubiquitin protein probed with H5099-16.
Mouse Anti-His-Tag Monoclonal Antibody – Cat# MBS6268840

Product References and Citations for anti-His-Tag antibody

1. Conrath, K., et al., J. Biol. Chem. 276: 7346-7350 (2001). 2. Suen, J.L., et al., Immunol. 103: 301-309 (2001). 3. Hoffmann, S.C., et al., J. Biol. Chem. 282: 22,370-22,375 (2007). 4. Zheng, J., et al., BMC Immunol. 8: 4 (2007). 5. Bahi, A. & Dreyer, J.L., Genes Brain Behav. 7: 244-256 (2008). 6. Wrighton, K.H., et al., J. Biol. Chem. 284: 9755-9763 (2009). 7. Diefenbacher, M., et al., J. Biol. Chem. 286: 25,027-25,038 (2011). 8. Alvarez, M.M., et al., PLoS One 5: e10176 (2010). 9. Bahi, A., et al., Neuropsychopharmacology 33: 2726-2734 (2008). 10. Gunnarsen, K.S., et al., BMC Biotechnol. 10: 8 (2010). 11. Hwang, H.Y., et al., Mol. Immunol. 45: 2570-2580 (2008). 12. De Vooght, L. et al. (2012) Microb Cell Fact. 11: 23. 13. Saerens, D. et al. (2004) J Biol Chem. 279 (50): 51965-72. 14. Than, N.G. et al. (2014) Placenta. 35: 855-65.

Other Notes

Small volumes of anti-His-Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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