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Class 2, I-Ak/S (MHC) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6269000

MBS6269000 is an antibody targeting MHC Class 2, I-Ak/s. Also known as MHC Class 2, I-Ak/s (MHC Class II) (APC). Reactive in Mouse, Rat. Host: Mouse. More details

$905 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MHC Class 2, I-Ak/s, Monoclonal Antibody
Full Product Name
MHC Class 2, I-Ak/s (MHC Class II) (APC)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10F125
Host
Mouse
Species Reactivity
Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes a monomorphic determinant of the rat MHC class II I-Ak/s antigen present on B lymphocytes, dendric cells, some macrophages and certain epithelial cells. Does not react with the rat BDIX strain due to a defect in RT1B expression. Crossreacts with with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Species Crossreactivity: mouse
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Immunogen
Rat thymocyte membrane glycoprotein
Conjugate
APC

Product Categories/Family for anti-MHC Class 2, I-Ak/s antibody

Applications Tested/Suitable for anti-MHC Class 2, I-Ak/s antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)

Immunohistochemistry (IHC) of anti-MHC Class 2, I-Ak/s antibody

M3887-54A used for the identification of MHC class II expressing microglial cells in rat brain by immunohistochemistry on formalin fixed free foating sections. MHC II+cells in the corpus callosum (CC) after tMCAO. Temporal expression of MHC II in the CC contralateral and ipsilateral to the lesioned hemisphere at different time points after tMCAO and a sham operated animal, respectively. Scale bars: lower magnification - 100uM; higher magnification - 20uM. From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain--influence of levodopa treatment..
Mouse Anti-MHC Monoclonal Antibody – Cat# MBS6269000

Immunohistochemistry (IHC) of anti-MHC Class 2, I-Ak/s antibody

M3887-54A used for the identification of activated microglia in rat brain by immunohistochemistry on formalin fixed free-floating sections. Spatiotemporal expression of MHC II protein in the ischemic hemisphere. Spatiotemporal distribution of MHC II expressing cells in the ipsilateral cortex of a sham operated animal and at the indicated time points after tMCAO. The border of the infarct core is delineated by a dotted line. Scale bars: low magnification - 200um; higher magnification - 20um. Abbreviations: V, lateral ventricle; IC, infarct core.
Mouse Anti-MHC Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6269000

Testing Data of anti-MHC Class 2, I-Ak/s antibody

Staining of rat spleen cells withMBS607599.
Mouse Anti-MHC Monoclonal Antibody, Testing Data – Cat# MBS6269000

Testing Data of anti-MHC Class 2, I-Ak/s antibody

A488 conjugated mouse anti rat CD45RA and A647 conjugated mouse IgG1 isotype control. Figure B. A488 conjugated mouse anti rat CD45RA and A647 conjugated mouse anti rat MHC-II. All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Mouse Anti-MHC Monoclonal Antibody, Testing Data – Cat# MBS6269000

Product References and Citations for anti-MHC Class 2, I-Ak/s antibody

1. McMaster, W. R. and Williams, A. F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Eur. J. Immunol. 9: 426-433. 2. Fernandez, J. L. and Weeks, M. (1986) Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens. Lab. Anim. 20: 293-297. 3. Charteris, D. G. and Lightman, S. L. (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis. Immunology. 78: 387-392. 4. Whiteland, J. L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320. 5. McKechnie, N. M. et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia. J. Infect. Dis. 176: 1334-1343. 6. Male, D. K. et al. (1987) Serological evidence for a defect in RT1.B (I-A) expression by the BDIX rat strain J. Immunogenet. 14: 301-312. 7. Meyer zu Horste, G. et al. (2011) Quinpramine ameliorates rat experimental autoimmune neuritis and redistributes MHC class II molecules. PLoS One. 6(6): e21223.

Other Notes

Small volumes of anti-MHC Class 2, I-Ak/s antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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