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Neurofilament, Medium Polypeptide (NEFM) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626981

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and… More details

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Product Overview

Online Datasheet in PDF
Product Name
NEFM, Neurofilament, Medium Polypeptide, Polyclonal Antibody
Full Product Name
NEFM, Neurofilament, Medium Polypeptide
Product Gene Name
Product Synonym Names
Anti -NEFM, Neurofilament, Medium Polypeptide
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by peptide affinity chromatography.
Specificity
Recognizes NEFM. Species crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
102,472 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (24770712..24776607). Location: 8p21
OMIM
162250
3D Structure
ModBase 3D Structure for P07197
Immunogen
Synthetic peptide corresponding to C-QASHITVERKDYLK, from the internal region of human NEFM (NP_005373.2; NP_001099011.1).

Product Categories/Family for anti-NEFM antibody

Applications Tested/Suitable for anti-NEFM antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-NEFM antibody
Suitable for use in ELISA and Western Blot.
Dilution: Peptide ELISA Titer: 1:128,000
Western Blot: 0.03-0.1ug/ml detects ~170-200kD band observed in Mouse Brain lysates (calculated MW of 102kD according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.

Chemiluminescence of anti-NEFM antibody

MBS626981 (0.03ug/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NEFM Polyclonal Antibody – Cat# MBS626981

Other Notes

Small volumes of anti-NEFM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEFM purchase

MBS626981 is an antibody targeting NEFM. Also known as NEFM, Neurofilament, Medium Polypeptide. Reactive in Human, Mouse. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for NEFM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neurofilament medium polypeptide isoform 1
NCBI Official Synonym Full Names
neurofilament, medium polypeptide
NCBI Official Symbol
NEFM
NCBI Official Synonym Symbols
NFM; NEF3; NF-M
UniProt Protein Name
Neurofilament medium polypeptide
UniProt Synonym Protein Names
160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein
UniProt Gene Name
NEFM
UniProt Synonym Gene Names
NEF3; NFM; NF-M
UniProt Entry Name
NFM_HUMAN
NCBI Protein Information
neurofilament medium polypeptide; neurofilament 3; 160 kDa neurofilament protein; neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide 150kDa

UniProt Comments for NEFM

NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: axon; neurofilament; neuromuscular junction

Molecular Function: protein binding; structural constituent of cytoskeleton; microtubule binding

Biological Process: neurofilament bundle assembly; regulation of axon diameter; axon cargo transport; microtubule cytoskeleton organization and biogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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