Rabbit Anti-MRNA Polyclonal Antibody – Cat# MBS6269823

MRNA Antibody – Rabbit Polyclonal

Name: MRNA Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6269823
Price: 1105 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6269823

MBS6269823 is an antibody targeting MRNA. Also known as MRNA (mRNA-capping Enzyme, HCAP1, HCE, RNGTT, CAP1A) (MaxLight 750). Reactive in Human. Host: Rabbit. More details

$1105 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MRNA, Polyclonal Antibody

Full Product Name

MRNA (mRNA-capping Enzyme, HCAP1, HCE, RNGTT, CAP1A) (MaxLight 750)

Product Gene Name

anti-MRNA antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

Purity/Purification

Purified by Immunoaffinity chromatography.

Specificity

Recognizes human MRNA. Species sequence homology: Mouse

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

70.9 kDa (620aa)

OMIM

603512

3D Structure

ModBase 3D Structure for O60942

Immunogen

Recombinant corresponding to human MRNA fused to GST-tag.

Conjugate

MaxLight750

Positive Control

HeLa nuclear extract

Note

Preservative Free

Product Categories/Family for anti-MRNA antibody

Antibodies; Enzymes

Applications Tested/Suitable for anti-MRNA antibody

Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-MRNA antibody

ICC: 1:500
WB: 1:5000
Applications are based on unconjugated antibody.

Product References and Citations for anti-MRNA antibody

1. Cowling, V.H. and Cole, M.D. (2009). Oncogene. Doi:10.1038/onc.2008.463. Analysis 2. Cowling, V.H. and Cole, M.D. (2008). Nat Rev Mol Cell Biol. Doi: 10.1038/ nrm2467 3. Bentley, D.L. (2005). Curr Opin Cell Biol. 17:251-256.

Other Notes

Small volumes of anti-MRNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MRNA. It may not necessarily be applicable to this product.

NCBI GI #

134142828

NCBI GeneID

8732

NCBI Accession #

NP_003791

UniProt Primary Accession #

O60942

UniProt Secondary Accession #

O43483; O60257; O60351; Q5TCW8; Q8WUM8; E1P513; E1P514

UniProt Related Accession #

O60942

GenBank Nucleotide #

NM_003800.4

NCBI Official Full Name

mRNA-capping enzyme isoform a

NCBI Official Synonym Full Names

RNA guanylyltransferase and 5'-phosphatase

NCBI Official Symbol

RNGTT

NCBI Official Synonym Symbols

HCE; HCE1; hCAP; CAP1A

UniProt Protein Name

mRNA-capping enzyme

UniProt Synonym Protein Names

HCAP1; HCEIncluding the following 2 domains:Polynucleotide 5'-triphosphatase (EC:3.1.3.33)Alternative name(s):mRNA 5'-triphosphatase; TPase

Protein Family

mRNA 3'-end-processing protein

UniProt Gene Name

RNGTT

UniProt Synonym Gene Names

CAP1A; TPase; GTase

UniProt Entry Name

MCE1_HUMAN

NCBI Protein Information

mRNA-capping enzyme

UniProt Comments for MRNA

RNGTT: a bifunctional mRNA capping enzyme with RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Four alternatively spliced human isoforms have been described. Isoform 1 and isoform 4 are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle. Isoforms 2, 3 and 4 lack mRNA 5'-guanylyltransferase activity due to disruptions of the GTPase domain.

Protein type: EC 2.7.7.50; EC 3.1.3.33; Motility/polarity/chemotaxis; Phosphatase (non-protein); Transferase

Chromosomal Location of Human Ortholog: 6q16

Cellular Component: nucleoplasm; nucleus

Molecular Function: mRNA guanylyltransferase activity; polynucleotide 5'-phosphatase activity; RNA guanylyltransferase activity; triphosphatase activity

Biological Process: mRNA capping; RNA processing; transcription from RNA polymerase II promoter

Research Articles on MRNA

1. These results identify a new domain of CE that is specific to its function in cytoplasmic capping, and a new role for Nck1 in regulating gene expression through its role as the scaffold for assembly of the cytoplasmic capping complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MRNA antibody

Reference Product	PubMed Publications
RNMT antibody	>5 publications with MRNA and RNMT

Pathways associated with anti-MRNA antibody

Products by Pathway	Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
HIV Infection Pathway antibodies	HIV Infection Pathway Diagram
HIV Life Cycle Pathway antibodies	HIV Life Cycle Pathway Diagram
Infectious Disease Pathway antibodies	Infectious Disease Pathway Diagram
Late Phase Of HIV Life Cycle Pathway antibodies	Late Phase Of HIV Life Cycle Pathway Diagram
RNA Pol II CTD Phosphorylation And Interaction With CE Pathway antibodies	RNA Pol II CTD Phosphorylation And Interaction With CE Pathway Diagram
RNA Pol II CTD Phosphorylation And Interaction With CE During HIV Infection Pathway antibodies	RNA Pol II CTD Phosphorylation And Interaction With CE During HIV Infection Pathway Diagram
RNA Polymerase II Transcription Pathway antibodies	RNA Polymerase II Transcription Pathway Diagram
Transcription Of The HIV Genome Pathway antibodies	Transcription Of The HIV Genome Pathway Diagram

Organs/Tissues associated with anti-MRNA antibody

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>1 publications with MRNA and Eye