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Myosin IIA (MYH9) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6269832

Myosin-2A heavy chain (MYH9) phosphorylation is known to be very important in regulating cell motility and chemotaxis. The suppression of myosin-2A heavy chain expression is strongly associated with osteoclast fusion and multinucleation. Recent research… More details

$950 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Myosin IIA (MYH9), Polyclonal Antibody
Full Product Name
Myosin IIA, Non-muscle, Phosphorylated (Ser1943) (Myosin-9, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-Muscle IIa, Non-Muscle Myosin Heavy Chain IIa, NMMHC II-a, NMMHC-IIA, Cellular Myosin Heavy Chain, Type A, Non-Muscle Myosin Heavy Chain A, NMMHC-A
Product Gene Name
anti-MYH9 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Purity/Purification
Purified by affinity chromatography.
Specificity
Recognizes human Myosin IIA, phosphorylated at Ser1943 at ~260kD. Two isoforms are produced by alternative splicing: isoform 1 at ~226kD and isoform 2 at ~160kD.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
159,864 Da
OMIM
153640
3D Structure
ModBase 3D Structure for P35579
Immunogen
Synthetic linear peptide corresponding to human Myosin IIA, phosphorylated at Ser1943, conjugated to KLH(Accession# NP_002464). Species sequence homology: Rhesus macaque; 100%, canine; 91%, porcine; 90%, rat and mouse; 80%
Conjugate
MaxLight550
Positive Control
Lambda phosphastase-untreated and lambda phosphastase-treated MDA-MB-468 cell lysates.
Antigen Modification
Phosphorylated
Note
Preservative Free

Product Categories/Family for anti-MYH9 antibody

Antibodies; Myosin
Applications Tested/Suitable for anti-MYH9 antibody
Western Blot (WB), Immunocytochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-MYH9 antibody
Applications are based on unconjugated antibody.

Product References and Citations for anti-MYH9 antibody

1. Morimura, S., et al. (2011). Biochem Biophys Res Commun. 404(3):834-840. 2. Kopp, J.B., et al. (2010). Semin Nephrol. 30(4): 409-417. 3. McMichael, B.K., et al. (2009). J Biol Chem. 284(18):12266-12275. 4. Dulyaninova, N.G., et al. (2007). Mol Biol Cell. 18:3144-3155.

Other Notes

Small volumes of anti-MYH9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYH9 purchase

MBS6269832 is an antibody targeting MYH9. Also known as Myosin IIA, Non-muscle, Phosphorylated (Ser1943) (Myosin-9, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-Muscle IIa, Non-Muscle Myosin Heavy Chain IIa, NMMHC II-a, NMMHC-IIA, Cellular Myosin Heavy Chain, Type A, Non-Muscle Myosin Heavy Chain A, NMMHC-A. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MYH9. It may not necessarily be applicable to this product.

NCBI GI #
12667788
NCBI GeneID
4627
NCBI Accession #
NP_002464
UniProt Primary Accession #
P35579
UniProt Secondary Accession #
O60805; Q60FE2; Q86T83; A8K6E4
UniProt Related Accession #
P35579
GenBank Nucleotide #
NM_002473
NCBI Official Full Name
myosin-9
NCBI Official Synonym Full Names
myosin, heavy chain 9, non-muscle
NCBI Official Symbol
MYH9
NCBI Official Synonym Symbols
MHA; FTNS; EPSTS; BDPLT6; DFNA17; NMMHCA; NMHC-II-A; NMMHC-IIA
UniProt Protein Name
Myosin-9
UniProt Synonym Protein Names
Cellular myosin heavy chain, type A; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Non-muscle myosin heavy chain A; NMMHC-A; Non-muscle myosin heavy chain IIa
Protein Family
Myosin
UniProt Gene Name
MYH9
UniProt Synonym Gene Names
NMMHC-A; NMMHC II-a; NMMHC-IIA
UniProt Entry Name
MYH9_HUMAN
NCBI Protein Information
myosin-9; cellular myosin heavy chain, type A; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A

UniProt Comments for MYH9

MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cortical cytoskeleton; signalosome; protein complex; myosin II complex; leading edge; contractile ring; immunological synapse; actomyosin; cytosol; actin cytoskeleton; ruffle; cell-cell adherens junction; membrane; cytoplasm; plasma membrane; stress fiber; spindle; uropod; integrin complex; neuromuscular junction; nucleus; cleavage furrow

Molecular Function: actin filament binding; calmodulin binding; microfilament motor activity; protein binding; protein homodimerization activity; protein anchor; ATPase activity; motor activity; actin binding; ADP binding; actin-dependent ATPase activity; ATP binding

Biological Process: integrin-mediated signaling pathway; axon guidance; blood vessel endothelial cell migration; monocyte differentiation; actin filament-based movement; in utero embryonic development; actomyosin structure organization and biogenesis; membrane protein ectodomain proteolysis; cytokinesis; uropod organization and biogenesis; regulation of cell shape; establishment of meiotic spindle localization; protein transport; actin cytoskeleton reorganization; ephrin receptor signaling pathway; establishment of T cell polarity; angiogenesis; platelet formation; leukocyte migration; myoblast fusion; meiotic spindle organization and biogenesis

Disease: Deafness, Autosomal Dominant 17; May-hegglin Anomaly; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; Epstein Syndrome; Sebastian Syndrome
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MYH9 antibody

 Reference Product  PubMed Publications
 MYH10 antibody  >9 publications with MYH9 and MYH10
 S100A4 antibody  >5 publications with MYH9 and S100A4
 MYH11 antibody  >4 publications with MYH9 and MYH11
 MYL9 antibody  >3 publications with MYH9 and MYL9
 MYL12A antibody  >1 publications with MYH9 and MYL12A
 MYL12B antibody  >1 publications with MYH9 and MYL12B
 MYL6 antibody  >1 publications with MYH9 and MYL6

Pathways associated with anti-MYH9 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHA-mediated Growth Cone Collapse Pathway antibodies  EPHA-mediated Growth Cone Collapse Pathway Diagram
 Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies  Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway antibodies  Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway Diagram

Diseases associated with anti-MYH9 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >131 publications with MYH9 and Kidney Diseases
 MYH9-Related Disorders Antibodies  >53 publications with MYH9 and MYH9-Related Disorders
 Congenital Abnormalities Antibodies  >45 publications with MYH9 and Congenital Abnormalities
 Immune System Diseases Antibodies  >42 publications with MYH9 and Immune System Diseases
 Glomerulosclerosis, Focal Segmental Antibodies  >37 publications with MYH9 and Glomerulosclerosis, Focal Segmental
 Cardiovascular Diseases Antibodies  >36 publications with MYH9 and Cardiovascular Diseases
 Kidney Failure, Chronic Antibodies  >34 publications with MYH9 and Kidney Failure, Chronic
 Proteinuria Antibodies  >31 publications with MYH9 and Proteinuria
 Nephritis, Hereditary Antibodies  >29 publications with MYH9 and Nephritis, Hereditary
 Neoplasms Antibodies  >26 publications with MYH9 and Neoplasms

Organs/Tissues associated with anti-MYH9 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >171 publications with MYH9 and Blood
 Kidney Antibodies  >122 publications with MYH9 and Kidney
 Muscle Antibodies  >103 publications with MYH9 and Muscle
 Bone Antibodies  >22 publications with MYH9 and Bone
 Vascular Antibodies  >19 publications with MYH9 and Vascular
 Heart Antibodies  >18 publications with MYH9 and Heart
 Lung Antibodies  >17 publications with MYH9 and Lung
 Bone Marrow Antibodies  >17 publications with MYH9 and Bone Marrow
 Ear Antibodies  >12 publications with MYH9 and Ear
 Brain Antibodies  >11 publications with MYH9 and Brain
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