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Vesicular Monoamine Transporter 1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626988

MBS626988 is an antibody targeting Vesicular Monoamine Transporter 1. Also known as Anti-Vesicular Monoamine Transporter 1 (VMAT1). Reactive in Human. Host: Rabbit. More details

$905 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Vesicular Monoamine Transporter 1, Polyclonal Antibody
Full Product Name
Anti-Vesicular Monoamine Transporter 1 (VMAT1)
Product Synonym Names
VMAT1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid, neat serum, 40% glycerol.
Purity/Purification
Serum
Specificity
The human sequence does not show any appreciable homology with rat VMAT1 (1). Antibodies to hVMAT1 are not expected to crossreact with rVMAT1. Actual crossreactivity of antibodies in various species is not established
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Immunogen
A 20 AA peptide sequence near the C-terminus of human VMAT1 (1). This peptide is predicted to be cytoplasmic. The peptide was synthesized with amino terminal Cysteine, coupled to KLH
Source Information
Human

Product Categories/Family for anti-Vesicular Monoamine Transporter 1 antibody

Abs to Transporters
Applications Tested/Suitable for anti-Vesicular Monoamine Transporter 1 antibody
ELISA; Western Blot
Application Notes for anti-Vesicular Monoamine Transporter 1 antibody
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry: Not tested. We recommend the use of affinity purified antibody at 1-20ug/ml in paraformaldehyde fixed sections of tissues. Neat serum can be used at 1:500 or more. VMAT1 has been localized in adrenal chromaffin cells (2). VMAT1 was been found in endosomes of transfected CHO cells., and in LDCVs of rat PC12 cells (2). Adsorption of antibody with the antigenic peptide abolished immunoreactivity (2). VAMT1 has been localized in endocrine and paracrine cells associated with the intestine, stomach, sympathetic nervous system (3).
Recommended Dilutions: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.

Other Notes

Small volumes of anti-Vesicular Monoamine Transporter 1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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