Goat Anti-SDHAF1 Polyclonal Antibody – Cat# MBS627158

MBS627158 (0.3ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Succinate Dehydrogenase Complex Assembly Factor 1, SDHAF1 (SDHAF1) Antibody – Goat Polyclonal

Name: SDHAF1 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS627158
Price: 835 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627158

The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically… More details

$835 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Succinate Dehydrogenase Complex Assembly Factor 1, SDHAF1, Polyclonal Antibody

Full Product Name

Succinate Dehydrogenase Complex Assembly Factor 1, SDHAF1

Product Gene Name

anti-SDHAF1 antibody

Product Synonym Names

Anti -Succinate Dehydrogenase Complex Assembly Factor 1, SDHAF1

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Human

Form/Format

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Purity/Purification

Affinity Purified
Purified by peptide affinity chromatography.

Specificity

Recognizes human SDHAF1. Species sequence homology: mouse and canine.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

12,806 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (36486090..36487220). Location: 19q13.12

OMIM

252011

3D Structure

ModBase 3D Structure for A6NFY7

Immunogen

Synthetic peptide corresponding to C-HDSTGAPETRPDGR, from the C-Terminus of human SDHAF1 (NP_001036096.1).

Product Categories/Family for anti-SDHAF1 antibody

Antibodies; Abs to Proteins

Applications Tested/Suitable for anti-SDHAF1 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-SDHAF1 antibody

Suitable for use in ELISA and Western Blot.
Dilution: Peptide ELISA Titer: 1:16,000
Western Blot: 0.3-1ug/ml detects ~19kD band observed in Human Brain (Cerebellum) lysates (calculated MW of 12.8kD according to NP_001036096.1).

Other Notes

Small volumes of anti-SDHAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SDHAF1 purchase

MBS627158 is an antibody targeting SDHAF1. Also known as Succinate Dehydrogenase Complex Assembly Factor 1, SDHAF1. Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for SDHAF1. It may not necessarily be applicable to this product.

NCBI GI #

111038124

NCBI GeneID

644096

NCBI Accession #

NP_001036096.1

UniProt Primary Accession #

A6NFY7

UniProt Secondary Accession #

B2RPM7

UniProt Related Accession #

A6NFY7

GenBank Nucleotide #

NM_001042631.2

NCBI Official Full Name

succinate dehydrogenase assembly factor 1, mitochondrial

NCBI Official Synonym Full Names

succinate dehydrogenase complex assembly factor 1

NCBI Official Symbol

SDHAF1

NCBI Official Synonym Symbols

LYRM8

UniProt Protein Name

Succinate dehydrogenase assembly factor 1, mitochondrial

UniProt Synonym Protein Names

LYR motif-containing protein 8

UniProt Gene Name

SDHAF1

UniProt Synonym Gene Names

SDH assembly factor 1

UniProt Entry Name

SDHF1_HUMAN

NCBI Protein Information

succinate dehydrogenase assembly factor 1, mitochondrial; SDH assembly factor 1; LYR motif containing 8; LYR motif-containing protein 8

UniProt Comments for SDHAF1

SDHAF1: Plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II (Probable). Defects in SDHAF1 are a cause of mitochondrial complex II deficiency (MT-C2D); also known as SDH-defective infantile leukoencephalopathy. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome. Belongs to the complex I LYR family. SDHAF1 subfamily.

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: mitochondrion; mitochondrial matrix

Biological Process: iron-sulfur cluster assembly

Disease: Mitochondrial Complex Ii Deficiency

Research Articles on SDHAF1

1. Studies indicate that an array of tumor syndromes caused by complex II-associated mutations in genes SDHA, SDHB, SDHC, SDHD, SDHAF1 and SDHAF2 have been identified over a decade.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SDHAF1 antibody

Reference Product	PubMed Publications
SDHA antibody	>5 publications with SDHAF1 and SDHA
ROMO1 antibody	>1 publications with SDHAF1 and ROMO1

Diseases associated with anti-SDHAF1 antibody

Disease Name	Pubmed Publications
Mitochondrial Complex II Deficiency Antibodies	>1 publications with SDHAF1 and Mitochondrial Complex II Deficiency

Organs/Tissues associated with anti-SDHAF1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>2 publications with SDHAF1 and Muscle
Brain Antibodies	>2 publications with SDHAF1 and Brain
Blood Antibodies	>1 publications with SDHAF1 and Blood
Kidney Antibodies	>1 publications with SDHAF1 and Kidney