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Phosphoglycerate Kinase 2 (PGK2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627235

PGK2 is a form of phosphoglycerate kinase unique to spermatozoa. The autosomal PGK2 gene lacks introns and contains characteristics of a processed gene. The conservation of function in this processed PGK2 gene and its tissue-specific expression in… More details

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Product Overview

Online Datasheet in PDF
Product Name
Phosphoglycerate Kinase 2 (PGK2), Polyclonal Antibody
Full Product Name
Phosphoglycerate Kinase 2, NT (PGK2, dJ417L20.2, PGKB, PGKPS, Phosphoglycerate Kinase Testis Specific)
Product Gene Name
anti-PGK2 antibody
Product Synonym Names
Anti -Phosphoglycerate Kinase 2, NT (PGK2, dJ417L20.2, PGKB, PGKPS, Phosphoglycerate Kinase Testis Specific)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human PGK2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
44,796 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (49753364..49755053, complement). Location: 6p12.3
OMIM
172270
3D Structure
ModBase 3D Structure for P07205
Immunogen
Synthetic peptide selected from the N-terminal region of human PGK2 (KLH).

Product Categories/Family for anti-PGK2 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-PGK2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PGK2 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-PGK2 antibody

Western blot analysis of anti-PGK2 in HepG2 cell line lysate (35ug/lane).PGK2(arrow) was detected using the purified Pab.
Rabbit Anti-PGK2 Polyclonal Antibody – Cat# MBS627235

Immunohistochemistry (IHC) of anti-PGK2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-PGK2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627235

Immunohistochemistry (IHC) of anti-PGK2 antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with PGK2 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PGK2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627235

Other Notes

Small volumes of anti-PGK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGK2 purchase

MBS627235 is an antibody targeting PGK2. Also known as Phosphoglycerate Kinase 2, NT (PGK2, dJ417L20.2, PGKB, PGKPS, Phosphoglycerate Kinase Testis Specific). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PGK2. It may not necessarily be applicable to this product.

NCBI GI #
31543397
NCBI GeneID
5232
NCBI Accession #
NP_620061.2
UniProt Primary Accession #
P07205
UniProt Secondary Accession #
Q9H107; B2R6Y8
UniProt Related Accession #
P07205
GenBank Nucleotide #
NM_138733.4
NCBI Official Full Name
phosphoglycerate kinase 2
NCBI Official Synonym Full Names
phosphoglycerate kinase 2
NCBI Official Symbol
PGK2
NCBI Official Synonym Symbols
PGKB; PGKPS; dJ417L20.2
UniProt Protein Name
Phosphoglycerate kinase 2
UniProt Synonym Protein Names
Phosphoglycerate kinase, testis specific
Protein Family
2-phosphoglycerate kinase
UniProt Gene Name
PGK2
UniProt Synonym Gene Names
PGKB
UniProt Entry Name
PGK2_HUMAN
NCBI Protein Information
phosphoglycerate kinase 2; OTTHUMP00000016591; phosphoglycerate kinase, testis specific; phosphoglycerate kinase autosomal pseudogene

NCBI Summary for PGK2

This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.
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UniProt Comments for PGK2

PGK2: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]

Protein type: EC 2.7.2.3; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: cytoplasm; nucleus

Molecular Function: phosphoglycerate kinase activity; ATP binding

Biological Process: sperm motility; glycolysis; phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PGK2 antibody

 Products by Pathway  Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis And Gluconeogenesis Pathway antibodies  Glycolysis And Gluconeogenesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with anti-PGK2 antibody

 Disease Name  Pubmed Publications
 Shock Antibodies  >1 publications with PGK2 and Shock
 Neuromuscular Diseases Antibodies  >1 publications with PGK2 and Neuromuscular Diseases
 Fetal Diseases Antibodies  >1 publications with PGK2 and Fetal Diseases
 Metabolic Diseases Antibodies  >1 publications with PGK2 and Metabolic Diseases
 Digestive System Diseases Antibodies  >1 publications with PGK2 and Digestive System Diseases

Organs/Tissues associated with anti-PGK2 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >28 publications with PGK2 and Testis
 Brain Antibodies  >2 publications with PGK2 and Brain
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