• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ALG10 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6272871

ALG10 encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ALG10, Polyclonal Antibody
Full Product Name
ALG10, NT (ALG10, ALG10A, Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase, Alpha-1,2-glucosyltransferase ALG10-A, Alpha-2-glucosyltransferase ALG10-A, Asparagine-linked glycosylation protein 10 homolog A) (PE)
Product Gene Name
anti-ALG10 antibody
Product Synonym Names
ALG10; ALG10A; Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase; Alpha-1,2-glucosyltransferase ALG10-A; Alpha-2-glucosyltransferase ALG10-A; Asparagine-linked glycosylation protein 10 homolog A
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
55,606 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (34175216..34181237). Location: 12p11.1
3D Structure
ModBase 3D Structure for Q5BKT4
Immunogen
ALG10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23~52 amino acids from the N-terminal region of human ALG10.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-ALG10 antibody

Antibodies; Enzymes
Applications Tested/Suitable for anti-ALG10 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-ALG10 antibody
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ALG10 antibody

Western blot analysis of ALG10 Antibody (N-term) in A375 cell line lysates (35ug/lane). ALG10 (arrow) was detected using the purified Pab.
Rabbit Anti-ALG10 Polyclonal Antibody – Cat# MBS6272871

Immunohistochemistry (IHC) of anti-ALG10 antibody

ALG10 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALG10 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-ALG10 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6272871

Product References and Citations for anti-ALG10 antibody

Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009). Seshadri, S., et al. BMC Med. Genet. 8 SUPPL 1, S15 (2007). Kupershmidt, S., et al. FASEB J. 17(15):2263-2265(2003).

Other Notes

Small volumes of anti-ALG10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALG10 purchase

MBS6272871 is an antibody targeting ALG10. Also known as ALG10, NT (ALG10, ALG10A, Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase, Alpha-1,2-glucosyltransferase ALG10-A, Alpha-2-glucosyltransferase ALG10-A, Asparagine-linked glycosylation protein 10 homolog A) (PE). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ALG10. It may not necessarily be applicable to this product.

NCBI GI #
60551132
NCBI GeneID
84920
NCBI Accession #
AAH90948.1
UniProt Primary Accession #
Q5BKT4
UniProt Secondary Accession #
Q6NS98; Q96DU0; Q96SM6
UniProt Related Accession #
Q5BKT4
NCBI Official Full Name
ALG10 protein
NCBI Official Synonym Full Names
ALG10, alpha-1,2-glucosyltransferase
NCBI Official Symbol
ALG10
NCBI Official Synonym Symbols
DIE2; KCR1; ALG10A
UniProt Protein Name
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
UniProt Synonym Protein Names
Alpha-1,2-glucosyltransferase ALG10-A; Alpha-2-glucosyltransferase ALG10-A; Asparagine-linked glycosylation protein 10 homolog A
Protein Family
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
UniProt Gene Name
ALG10
UniProt Synonym Gene Names
ALG10A
UniProt Entry Name
AG10A_HUMAN
NCBI Protein Information
dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase; dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase; alpha2-glucosyltransferase; potassium channel regulator 1; alpha-2-glucosyltransferase ALG10-A; alpha-1,2-glucosylt

NCBI Summary for ALG10

This gene encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel. [provided by RefSeq, Jan 2010]
Show more

UniProt Comments for ALG10

Function: Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol.

Catalytic activity: Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathway: Protein modification; protein glycosylation.

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the ALG10 glucosyltransferase family.

Sequence caution: The sequence BAB55272.1 differs from that shown. Reason: Erroneous initiation.
Show more

Research Articles on ALG10

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ALG10 antibody

 Reference Product  PubMed Publications
 ALG6 antibody  >2 publications with ALG10 and ALG6
 ALG8 antibody  >2 publications with ALG10 and ALG8
 KCNE2 antibody  >1 publications with ALG10 and KCNE2
 ALG5 antibody  >1 publications with ALG10 and ALG5
 KCNH2 antibody  >1 publications with ALG10 and KCNH2

Pathways associated with anti-ALG10 antibody

 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway antibodies  Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 N-Glycan Biosynthesis Pathway antibodies  N-Glycan Biosynthesis Pathway Diagram
 N-Glycan Biosynthesis Pathway antibodies  N-Glycan Biosynthesis Pathway Diagram
 N-glycan Precursor Biosynthesis Pathway antibodies  N-glycan Precursor Biosynthesis Pathway Diagram
 N-glycan Precursor Biosynthesis Pathway antibodies  N-glycan Precursor Biosynthesis Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By Wnt Pathway antibodies  Signaling By Wnt Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000