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UBE2B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627321

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s,… More details

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Product Overview

Online Datasheet in PDF
Product Name
UBE2B, Polyclonal Antibody
Full Product Name
UBE2B, CT (Ubiquitin-conjugating Enzyme E2 B, Ubiquitin-protein Ligase B, Ubiquitin Carrier Protein B, RAD6 Homolog B, HR6B, Ubiquitin-conjugating Enzyme E2-17kD, RAD6B)
Product Gene Name
anti-UBE2B antibody
Product Synonym Names
Anti -UBE2B, CT (Ubiquitin-conjugating Enzyme E2 B, Ubiquitin-protein Ligase B, Ubiquitin Carrier Protein B, RAD6 Homolog B, HR6B, Ubiquitin-conjugating Enzyme E2-17kD, RAD6B)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human UBE2B.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
17,312 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (133706870..133727799). Location: 5q31.1
OMIM
179095
3D Structure
ModBase 3D Structure for P63146
Immunogen
Synthetic peptide selected from the C-terminal region of human UBE2B (KLH).

Product Categories/Family for anti-UBE2B antibody

Antibodies; Abs to Ubiquitin Ligases
Applications Tested/Suitable for anti-UBE2B antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-UBE2B antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Immunohistochemistry (IHC) of anti-UBE2B antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-UBE2B Polyclonal Antibody – Cat# MBS627321

Western Blot (WB) of anti-UBE2B antibody

Western blot analysis of UBE2B Antibody (C-term) in Ramos cell line lysates (35ug/lane). UBE2B (arrow) was detected using the purified Pab.
Rabbit Anti-UBE2B Polyclonal Antibody, Western Blot (WB) – Cat# MBS627321

Western Blot (WB) of anti-UBE2B antibody

Western blot analysis of UBE2B (arrow) using rabbit polyclonal UBE2B Antibody (E132) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2B gene.
Rabbit Anti-UBE2B Polyclonal Antibody, Western Blot (WB) – Cat# MBS627321

Other Notes

Small volumes of anti-UBE2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBE2B purchase

MBS627321 is an antibody targeting UBE2B. Also known as UBE2B, CT (Ubiquitin-conjugating Enzyme E2 B, Ubiquitin-protein Ligase B, Ubiquitin Carrier Protein B, RAD6 Homolog B, HR6B, Ubiquitin-conjugating Enzyme E2-17kD, RAD6B). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2B. It may not necessarily be applicable to this product.

NCBI GI #
4507771
NCBI GeneID
7320
NCBI Accession #
NP_003328.1
UniProt Primary Accession #
P63146
UniProt Secondary Accession #
P23567; Q4PJ15; Q9D0J6; B2R503; D3DQA2
UniProt Related Accession #
P63146
GenBank Nucleotide #
NM_003337.2
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 B
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2B
NCBI Official Symbol
UBE2B
NCBI Official Synonym Symbols
HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 B
UniProt Synonym Protein Names
RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B
Protein Family
Ubiquitin-conjugating enzyme
UniProt Gene Name
UBE2B
UniProt Synonym Gene Names
RAD6B
UniProt Entry Name
UBE2B_HUMAN
NCBI Protein Information
ubiquitin-conjugating enzyme E2 B; E2 protein; RAD6 homolog B; OTTHUMP00000159383; OTTHUMP00000223112; OTTHUMP00000223113; ubiquitin-protein ligase B; ubiquitin carrier protein B; ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog)

NCBI Summary for UBE2B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]
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UniProt Comments for UBE2B

UBE2B: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: XY body; cytoplasm; nuclear chromatin; plasma membrane; replication fork; chromatin; nucleus

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; response to drug; protein monoubiquitination; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; protein stabilization; in utero embryonic development; postreplication repair; protein ubiquitination; Wnt receptor signaling pathway through beta-catenin; DNA repair; negative regulation of histone phosphorylation; cellular response to insulin stimulus; response to hypoxia; maintenance of chromatin silencing; sperm axoneme assembly; spermatogenesis; response to DNA damage stimulus; chiasma formation; histone H2A ubiquitination; negative regulation of apoptosis; response to UV
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Research Articles on UBE2B

1. Ubc2/Rad6 ser(120) regulates ubiquitin-dependent N-end rule targeting by E3{alpha}/Ubr1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UBE2B antibody

 Reference Product  PubMed Publications
 RAD18 antibody  >4 publications with UBE2B and RAD18
 UBR1 antibody  >3 publications with UBE2B and UBR1
 UBR2 antibody  >2 publications with UBE2B and UBR2

Pathways associated with anti-UBE2B antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Proteasome Degradation Pathway antibodies  Proteasome Degradation Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-UBE2B antibody

 Disease Name  Pubmed Publications
 Infertility, Male Antibodies  >5 publications with UBE2B and Infertility, Male
 Neoplasms Antibodies  >5 publications with UBE2B and Neoplasms
 Breast Neoplasms Antibodies  >3 publications with UBE2B and Breast Neoplasms
 Atrophy Antibodies  >2 publications with UBE2B and Atrophy
 Carcinoma Antibodies  >2 publications with UBE2B and Carcinoma
 Body Weight Changes Antibodies  >1 publications with UBE2B and Body Weight Changes
 Leukemia, Myeloid Antibodies  >1 publications with UBE2B and Leukemia, Myeloid
 Hyperplasia Antibodies  >1 publications with UBE2B and Hyperplasia
 Necrosis Antibodies  >1 publications with UBE2B and Necrosis
 Cachexia Antibodies  >1 publications with UBE2B and Cachexia
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