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ENOA (eno) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627408

ENO1 is one of three enolase isoenzymes found in mammals; the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length… More details

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Product Overview

Online Datasheet in PDF
Product Name
ENOA, Polyclonal Antibody
Full Product Name
ENOA, NT (Alpha-enolase, 2-phospho-D-glycerate Hydro-lyase, Non-neural Enolase, NNE, Enolase 1, Phosphopyruvate Hydratase, C-myc Promoter-binding Protein, MBP-1, MPB-1, Plasminogen-binding Protein, MPB1, MBPB1, ENO1L1, ENO1)
Product Gene Name
anti-eno antibody
Product Synonym Names
Anti -ENOA, NT (Alpha-enolase, 2-phospho-D-glycerate Hydro-lyase, Non-neural Enolase, NNE, Enolase 1, Phosphopyruvate Hydratase, C-myc Promoter-binding Protein, MBP-1, MPB-1, Plasminogen-binding Protein, MPB1, MBPB1, ENO1L1, ENO1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ENOA.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
46,912 Da
Chromosome Location
NC_009004.1 (606184..607485)
3D Structure
ModBase 3D Structure for A2RIX1
Immunogen
Synthetic peptide between aa39~69 from the N-terminal region of ENOA (KLH).

Product Categories/Family for anti-eno antibody

Antibodies; Abs to Cancer Markers
Applications Tested/Suitable for anti-eno antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-eno antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-eno antibody

ENOA Antibody (N-term) western blot analysis in U251,mouse C2C12,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENOA antibody detected the ENOA protein (arrow).
Rabbit Anti-eno Polyclonal Antibody – Cat# MBS627408

Flow Cytometry (FC/FACS) of anti-eno antibody

ENOA Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-eno Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS627408

Immunofluorescence (IF) of anti-eno antibody

Fluorescent confocal image of Hela cell stained with ENOA Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENOA primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ENOA immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Rabbit Anti-eno Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS627408

Other Notes

Small volumes of anti-eno antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for eno purchase

MBS627408 is an antibody targeting eno. Also known as ENOA, NT (Alpha-enolase, 2-phospho-D-glycerate Hydro-lyase, Non-neural Enolase, NNE, Enolase 1, Phosphopyruvate Hydratase, C-myc Promoter-binding Protein, MBP-1, MPB-1, Plasminogen-binding Protein, MPB1, MBPB1, ENO1L1, ENO1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for eno. It may not necessarily be applicable to this product.

NCBI GI #
124492286
NCBI GeneID
4797432
UniProt Primary Accession #
A2RIX1
UniProt Related Accession #
A2RIX1
NCBI Official Full Name
EnoA protein
NCBI Official Symbol
eno
UniProt Protein Name
Enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase
Protein Family
Enolase
UniProt Gene Name
eno
UniProt Entry Name
ENO_LACLM
NCBI Protein Information
phosphopyruvate hydratase

UniProt Comments for eno

Function: Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

By similarity. HAMAP MF_00318

Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O. HAMAP MF_00318

Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer

Enzyme regulation: The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein

By similarity. HAMAP MF_00318

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. HAMAP MF_00318

Subcellular location: Cytoplasm. Secreted. Cell surface. Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial cell surface

By similarity. HAMAP MF_00318

Sequence similarities: Belongs to the enolase family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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