• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TPI1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627441

TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TPI1, Polyclonal Antibody
Full Product Name
TPI1, CT (Triosephosphate Isomerase, TIM, Triose-phosphate Isomerase, TPI)
Product Gene Name
anti-TPI1 antibody
Product Synonym Names
Anti -TPI1, CT (Triosephosphate Isomerase, TIM, Triose-phosphate Isomerase, TPI)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.102% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human TPI1. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
26,669 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (6976584..6980110). Location: 12p13
OMIM
190450
3D Structure
ModBase 3D Structure for P60174
Immunogen
Synthetic peptide selected from the C-terminal region of human TPI1 (KLH).

Product Categories/Family for anti-TPI1 antibody

Antibodies; Abs to Enzymes; Isomerase
Applications Tested/Suitable for anti-TPI1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-TPI1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-TPI1 antibody

Western blot analysis of TPI1 Antibody (C-term) in Y79,CEM cell line and mouse brain tissue lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml)
Rabbit Anti-TPI1 Polyclonal Antibody – Cat# MBS627441

Immunohistochemistry (IHC) of anti-TPI1 antibody

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-TPI1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627441

Flow Cytometry (FC/FACS) of anti-TPI1 antibody

TPI1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-TPI1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS627441

Western Blot (WB) of anti-TPI1 antibody

Western blot analysis of TPI1 (arrow) using rabbit polyclonal TPI1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene.
Rabbit Anti-TPI1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS627441

Other Notes

Small volumes of anti-TPI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPI1 purchase

MBS627441 is an antibody targeting TPI1. Also known as TPI1, CT (Triosephosphate Isomerase, TIM, Triose-phosphate Isomerase, TPI). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TPI1. It may not necessarily be applicable to this product.

NCBI GI #
49456365
NCBI GeneID
7167
UniProt Primary Accession #
P60174
UniProt Secondary Accession #
P00938; Q6FHP9; Q6IS07; Q8WWD0; Q96AG5
UniProt Related Accession #
P60174; Q53HE2
NCBI Official Full Name
TPI1
NCBI Official Synonym Full Names
triosephosphate isomerase 1
NCBI Official Symbol
TPI1
NCBI Official Synonym Symbols
TIM; TPI; MGC88108
UniProt Protein Name
Triosephosphate isomerase
UniProt Synonym Protein Names
Triose-phosphate isomerase
UniProt Gene Name
TPI1
UniProt Synonym Gene Names
TPI
UniProt Entry Name
TPIS_HUMAN
NCBI Protein Information
triosephosphate isomerase; OTTHUMP00000165244; triose-phosphate isomerase

NCBI Summary for TPI1

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Show more

UniProt Comments for TPI1

TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; Carbohydrate Metabolism - inositol phosphate; Carbohydrate Metabolism - fructose and mannose; EC 5.3.1.1

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: extracellular space; nucleus; cytosol

Molecular Function: protein binding; triose-phosphate isomerase activity

Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis

Disease: Triosephosphate Isomerase Deficiency
Show more

Research Articles on TPI1

1. structural changes rather than abnormal catalysis may play an important role in the clinical manifestations of TPI deficiency; the postulated high aggregation ability of the unstable Glu104Asp mutant would lead to more serious symptoms

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TPI1 antibody

 Products by Pathway  Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis And Gluconeogenesis Pathway antibodies  Glycolysis And Gluconeogenesis Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram

Diseases associated with anti-TPI1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with TPI1 and Neoplasms
 Carcinoma Antibodies  >4 publications with TPI1 and Carcinoma
 Adenocarcinoma Antibodies  >3 publications with TPI1 and Adenocarcinoma
 Skin Diseases Antibodies  >2 publications with TPI1 and Skin Diseases
 Carcinoma, Ductal, Breast Antibodies  >2 publications with TPI1 and Carcinoma, Ductal, Breast
 Breast Neoplasms Antibodies  >2 publications with TPI1 and Breast Neoplasms
 Drug Toxicity Antibodies  >1 publications with TPI1 and Drug Toxicity
 Hypertension Antibodies  >1 publications with TPI1 and Hypertension
 Lung Diseases Antibodies  >1 publications with TPI1 and Lung Diseases
 Head and Neck Neoplasms Antibodies  >1 publications with TPI1 and Head and Neck Neoplasms

Organs/Tissues associated with anti-TPI1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with TPI1 and Blood
 Muscle Antibodies  >2 publications with TPI1 and Muscle
 Adipose Tissue Antibodies  >1 publications with TPI1 and Adipose Tissue
 Esophagus Antibodies  >1 publications with TPI1 and Esophagus
 Mouth Antibodies  >1 publications with TPI1 and Mouth
 Connective Tissue Antibodies  >1 publications with TPI1 and Connective Tissue
 Prostate Antibodies  >1 publications with TPI1 and Prostate
 Liver Antibodies  >1 publications with TPI1 and Liver
 Vascular Antibodies  >1 publications with TPI1 and Vascular
 Kidney Antibodies  >1 publications with TPI1 and Kidney
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000