• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

APOBEC3F Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6274449

This protein is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
APOBEC3F, Polyclonal Antibody
Full Product Name
APOBEC3F, NT (APOBEC3F, DNA dC->dU-editing enzyme APOBEC-3F, Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F) (Azide free) (HRP)
Product Gene Name
anti-APOBEC3F antibody
Product Synonym Names
APOBEC3F; DNA dC->dU-editing enzyme APOBEC-3F; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human APOBEC3F.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
373
OMIM
608993
3D Structure
ModBase 3D Structure for Q8IUX4
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 22-48 in the N-terminal region of human APOBEC3F. UniProt Accession #Q8IUX4
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-APOBEC3F antibody

Antibodies; Enzymes; Cytidine Deaminase (APOBEC)
Applications Tested/Suitable for anti-APOBEC3F antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-APOBEC3F antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-APOBEC3F antibody

Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS643632. APOBEC3F (arrow) was detected usingMBS643632.
Rabbit Anti-APOBEC3F Polyclonal Antibody – Cat# MBS6274449

Immunohistochemistry (IHC) of anti-APOBEC3F antibody

Immmunohistochemistry in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS643632, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS643632 for immunohistochemistry.
Rabbit Anti-APOBEC3F Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6274449

Product References and Citations for anti-APOBEC3F antibody

1. Murray, N.P., et al. Oncol. Rep. 24(3):687-692(2010). 2. Sonwalkar, S.A., et al. Histopathology 56(7):900-907(2010). 3. Lakis, S., et al. World J. Gastroenterol. 16(20):2476-2483(2010). 4. Chen, W., et al. Mol. Biol. Rep. 36(3):423-430(2009). 5. Mubiru, J.N., et al. Gene 327(1):89-98(2004).

Other Notes

Small volumes of anti-APOBEC3F antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOBEC3F purchase

MBS6274449 is an antibody targeting APOBEC3F. Also known as APOBEC3F, NT (APOBEC3F, DNA dC->dU-editing enzyme APOBEC-3F, Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for APOBEC3F. It may not necessarily be applicable to this product.

NCBI GI #
161784334
NCBI GeneID
200316
NCBI Accession #
Q8IUX4.3
UniProt Primary Accession #
Q8IUX4
UniProt Secondary Accession #
Q45F03; Q6ICH3; Q7Z2N2; Q7Z2N5; B0QYD4
UniProt Related Accession #
Q8IUX4
NCBI Official Full Name
DNA dC->
NCBI Official Synonym Full Names
apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F
NCBI Official Symbol
APOBEC3F
NCBI Official Synonym Symbols
A3F; KA6; ARP8; BK150C2.4.MRNA
UniProt Protein Name
DNA dC->dU-editing enzyme APOBEC-3F
UniProt Synonym Protein Names
Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F; A3F
Protein Family
DNA dC->dU-editing enzyme
UniProt Gene Name
APOBEC3F
UniProt Synonym Gene Names
A3F
UniProt Entry Name
ABC3F_HUMAN
NCBI Protein Information
DNA dC->dU-editing enzyme APOBEC-3F; induced upon T-cell activation; apolipoprotein B mRNA editing enzyme cytidine deaminase; apolipoprotein B editing enzyme catalytic polypeptide-like 3F; apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like

NCBI Summary for APOBEC3F

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for APOBEC3F

APOBEC3F: Host cellular restriction factor that may have antiviral activities against exogenous and endogenous viruses, as well as retrotransposons. After being packaged into HIV-1 virions, blocks productive infection by massively editing dC residues to dU on the DNA minus strand during reverse transcription. The editing of the minus strand DNA of HIV-1 during reverse transcription leads to G- to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Belongs to the cytidine and deoxycytidylate deaminase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; Hydrolase; EC 3.5.4.-

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: apolipoprotein B mRNA editing enzyme complex; cytoplasm; ribonucleoprotein complex

Molecular Function: protein binding; zinc ion binding; RNA binding; cytidine deaminase activity

Biological Process: negative regulation of retroviral genome replication; base conversion or substitution editing; negative regulation of viral genome replication; innate immune response; cytidine deamination; positive regulation of defense response to virus by host; negative regulation of viral reproduction; defense response to virus
Show more

Research Articles on APOBEC3F

1. Authors found that APOBEA3G, APOBEA3F, and APOBEA3H-hapII, but not APOBEA3D, were susceptible to HIV-2 Vif-induced degradation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-APOBEC3F antibody

 Reference Product  PubMed Publications
 APOBEC3G antibody  >168 publications with APOBEC3F and APOBEC3G
 APOBEC3C antibody  >24 publications with APOBEC3F and APOBEC3C
 APOBEC3D antibody  >14 publications with APOBEC3F and APOBEC3D
 CUL5 antibody  >11 publications with APOBEC3F and CUL5

Diseases associated with anti-APOBEC3F antibody

 Disease Name  Pubmed Publications
 Leukemia Antibodies  >9 publications with APOBEC3F and Leukemia
 Inflammation Antibodies  >4 publications with APOBEC3F and Inflammation
 Colorectal Neoplasms Antibodies  >2 publications with APOBEC3F and Colorectal Neoplasms
 Disease Models, Animal Antibodies  >2 publications with APOBEC3F and Disease Models, Animal
 Prostatic Neoplasms Antibodies  >2 publications with APOBEC3F and Prostatic Neoplasms
 Prostatic Diseases Antibodies  >2 publications with APOBEC3F and Prostatic Diseases
 Necrosis Antibodies  >1 publications with APOBEC3F and Necrosis
 Neoplasms, Experimental Antibodies  >1 publications with APOBEC3F and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >1 publications with APOBEC3F and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-APOBEC3F antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >37 publications with APOBEC3F and Blood
 Prostate Antibodies  >4 publications with APOBEC3F and Prostate
 Vascular Antibodies  >4 publications with APOBEC3F and Vascular
 Bone Marrow Antibodies  >3 publications with APOBEC3F and Bone Marrow
 Heart Antibodies  >2 publications with APOBEC3F and Heart
 Thymus Antibodies  >1 publications with APOBEC3F and Thymus
 Connective Tissue Antibodies  >1 publications with APOBEC3F and Connective Tissue
 Uncharacterized Tissue Antibodies  >1 publications with APOBEC3F and Uncharacterized Tissue
 Lymph Node Antibodies  >1 publications with APOBEC3F and Lymph Node
 Lymph Antibodies  >1 publications with APOBEC3F and Lymph
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000