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UBE1C (UBA3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627572

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s,… More details

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Product Overview

Online Datasheet in PDF
Product Name
UBE1C (UBA3), Polyclonal Antibody
Full Product Name
UBE1C, CT (Ubiquitin-activating Enzyme E1C, NEDD8-activating Enzyme E1 Catalytic Subunit, Ubiquitin-like Modifier-activating Enzyme 3, Ubiquitin-activating Enzyme 3, NEDD8-activating Enzyme E1C, UBA3)
Product Gene Name
anti-UBA3 antibody
Product Synonym Names
Anti -UBE1C, CT (Ubiquitin-activating Enzyme E1C, NEDD8-activating Enzyme E1 Catalytic Subunit, Ubiquitin-like Modifier-activating Enzyme 3, Ubiquitin-activating Enzyme 3, NEDD8-activating Enzyme E1C, UBA3)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human UBE1C. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
51,852 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (69103881..69129524, complement). Location: 3p24.3-p13
OMIM
603172
3D Structure
ModBase 3D Structure for Q8TBC4
Immunogen
Synthetic peptide selected from the C-terminal region of human UBE1C (KLH).

Product Categories/Family for anti-UBA3 antibody

Antibodies; Abs to Ubiquitin
Applications Tested/Suitable for anti-UBA3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-UBA3 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-UBA3 antibody

The anti-UBE1 C-term Pab is used in Western blot to detect UBE1 in mouse brain tissue lysate.
Rabbit Anti-UBA3 Polyclonal Antibody – Cat# MBS627572

Immunohistochemistry (IHC) of anti-UBA3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-UBA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627572

Western Blot (WB) of anti-UBA3 antibody

UBE1C Antibody (S430) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the UBE1C antibody detected the UBE1C protein (arrow).
Rabbit Anti-UBA3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS627572

Western Blot (WB) of anti-UBA3 antibody

Western blot analysis of UBE1C (arrow) using rabbit polyclonal UBE1C (S430) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE1C gene.
Rabbit Anti-UBA3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS627572

Other Notes

Small volumes of anti-UBA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBA3 purchase

MBS627572 is an antibody targeting UBA3. Also known as UBE1C, CT (Ubiquitin-activating Enzyme E1C, NEDD8-activating Enzyme E1 Catalytic Subunit, Ubiquitin-like Modifier-activating Enzyme 3, Ubiquitin-activating Enzyme 3, NEDD8-activating Enzyme E1C, UBA3). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBA3. It may not necessarily be applicable to this product.

NCBI GI #
49065500
NCBI GeneID
9039
UniProt Primary Accession #
Q8TBC4
UniProt Secondary Accession #
O76088; Q9NTU3; A6NLB5; A8K027
UniProt Related Accession #
Q8TBC4
NCBI Official Full Name
UBE1C
NCBI Official Synonym Full Names
ubiquitin-like modifier activating enzyme 3
NCBI Official Symbol
UBA3
NCBI Official Synonym Symbols
UBE1C; hUBA3; MGC22384; DKFZp566J164
UniProt Protein Name
NEDD8-activating enzyme E1 catalytic subunit
UniProt Synonym Protein Names
NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3
Protein Family
Ubiquitin-activating enzyme
UniProt Gene Name
UBA3
UniProt Synonym Gene Names
UBE1C
UniProt Entry Name
UBA3_HUMAN
NCBI Protein Information
NEDD8-activating enzyme E1 catalytic subunit; OTTHUMP00000205507; OTTHUMP00000205508; OTTHUMP00000205509; NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; ubiquitin-activating enzyme 3; ubiquitin-like modifier-activating enzyme 3; UBA3, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1C (UBA3 homolog, yeast); ubiquitin-activating enzyme E1C (homologous to yeast UBA3)

UniProt Comments for UBA3

UBA3: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Cell cycle regulation; Ubiquitin ligase; EC 6.3.2.-; Nuclear receptor co-regulator; Ubiquitin conjugating system; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: cytosol; nucleus

Molecular Function: NEDD8 activating enzyme activity; protein binding; acid-amino acid ligase activity; protein heterodimerization activity; ATP binding

Biological Process: protein neddylation; regulation of cell cycle; protein modification process; endomitotic cell cycle; proteolysis
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Research Articles on UBA3

1. X-ray crystallographic analysis of APPBP1-UBA3-NEDD8 shows that APPBP1-UBA3's preference for NEDD8's Ala72 appears to be indirect, due to proper positioning of UBA3's Arg190.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UBA3 antibody

 Reference Product  PubMed Publications
 NEDD8 antibody  >23 publications with UBA3 and NEDD8
 NAE1 antibody  >1 publications with UBA3 and NAE1

Pathways associated with anti-UBA3 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-UBA3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with UBA3 and Neoplasms
 Breast Neoplasms Antibodies  >1 publications with UBA3 and Breast Neoplasms
 Drug Toxicity Antibodies  >1 publications with UBA3 and Drug Toxicity
 Carcinoma Antibodies  >1 publications with UBA3 and Carcinoma
 Congenital Abnormalities Antibodies  >1 publications with UBA3 and Congenital Abnormalities
 Body Weight Changes Antibodies  >1 publications with UBA3 and Body Weight Changes

Organs/Tissues associated with anti-UBA3 antibody

 Organ/Tissue Name  Pubmed Publications
 Uterus Antibodies  >2 publications with UBA3 and Uterus
 Brain Antibodies  >2 publications with UBA3 and Brain
 Nerve Antibodies  >1 publications with UBA3 and Nerve
 Bone Marrow Antibodies  >1 publications with UBA3 and Bone Marrow
 Mammary Gland Antibodies  >1 publications with UBA3 and Mammary Gland
 Thymus Antibodies  >1 publications with UBA3 and Thymus
 Testis Antibodies  >1 publications with UBA3 and Testis
 Blood Antibodies  >1 publications with UBA3 and Blood
 Bone Antibodies  >1 publications with UBA3 and Bone
 Placenta Antibodies  >1 publications with UBA3 and Placenta
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