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MAGEA12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627686

MAGEA12 is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. This gene is expressed in various tumors and tumor cell lines from different… More details

$895 / 0.2 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MAGEA12, Polyclonal Antibody
Full Product Name
MAGEA12, NT (Melanoma-associated Antigen 12, MAGE-12 Antigen, MAGE-12F Antigen, Cancer/Testis Antigen 1.12, CT1.12, MAGE12)
Product Gene Name
Product Synonym Names
Anti -MAGEA12, NT (Melanoma-associated Antigen 12, MAGE-12 Antigen, MAGE-12F Antigen, Cancer/Testis Antigen 1.12, CT1.12, MAGE12)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human MAGEA12.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
34,836 Da
Chromosome Location
Chromosome: X; NC_000023.10 (151899293..151903184, complement). Location: Xq28
OMIM
300177
3D Structure
ModBase 3D Structure for P43365
Immunogen
Synthetic peptide selected from the N-terminal region of human MAGEA12 (KLH).
Applications Tested/Suitable for anti-MAGEA12 antibody
ELISA (EL/EIA), Immunohistochemistry (IHC)
Application Notes for anti-MAGEA12 antibody
Suitable for use in ELISA and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-MAGEA12 antibody

MAGEA12 Antibody (S96) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MAGEA12 antibody detected the MAGEA12 protein (arrow).
Rabbit Anti-MAGEA12 Polyclonal Antibody – Cat# MBS627686

Immunohistochemistry (IHC) of anti-MAGEA12 antibody

MAGEA12 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGEA12 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-MAGEA12 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627686

Other Notes

Small volumes of anti-MAGEA12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAGEA12 purchase

MBS627686 is an antibody targeting MAGEA12. Also known as MAGEA12, NT (Melanoma-associated Antigen 12, MAGE-12 Antigen, MAGE-12F Antigen, Cancer/Testis Antigen 1.12, CT1.12, MAGE12). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAGEA12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
melanoma-associated antigen 12
NCBI Official Synonym Full Names
melanoma antigen family A, 12
NCBI Official Symbol
MAGEA12
NCBI Official Synonym Symbols
CT1.12; MAGE12
UniProt Protein Name
Melanoma-associated antigen 12
UniProt Synonym Protein Names
Cancer/testis antigen 1.12; CT1.12; MAGE-12 antigen; MAGE12F antigen
UniProt Gene Name
MAGEA12
UniProt Synonym Gene Names
MAGE12
UniProt Entry Name
MAGAC_HUMAN
NCBI Protein Information
melanoma-associated antigen 12; MAGE-12 antigen; MAGE12F antigen; OTTHUMP00000024231; cancer/testis antigen 1.12; cancer/testis antigen family 1, member 12

NCBI Summary for MAGEA12

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]
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UniProt Comments for MAGEA12

MAGEA12: Not known, though may play a role tumor transformation or progression. In vitro promotes cell viability in melanoma cell lines.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq28

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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