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EIF4A1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627696

EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of… More details

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Product Overview

Online Datasheet in PDF
Product Name
EIF4A1, Polyclonal Antibody
Full Product Name
EIF4A1, NT (Eukaryotic Initiation Factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA Helicase eIF4A-1, DDX2A, EIF4A)
Product Gene Name
anti-EIF4A1 antibody
Product Synonym Names
Anti -EIF4A1, NT (Eukaryotic Initiation Factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA Helicase eIF4A-1, DDX2A, EIF4A)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human EIF4A1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
46,154 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (7476057..7482324). Location: 17p13
OMIM
602641
3D Structure
ModBase 3D Structure for P60842
Immunogen
Synthetic peptide selected from the N-terminal region of human EIF4A1 (KLH).

Product Categories/Family for anti-EIF4A1 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-EIF4A1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-EIF4A1 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50

Western Blot (WB) of anti-EIF4A1 antibody

Western blot analysis of anti-EIF4A1 Pab in K562 cell line lysates (35ug/lane). EIF4A1(arrow) was detected using the purified Pab.
Rabbit Anti-EIF4A1 Polyclonal Antibody – Cat# MBS627696

Western Blot (WB) of anti-EIF4A1 antibody

Western blot analysis of EIF4A1 (arrow) using rabbit polyclonal EIF4A1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4A1 gene (Lane 2) (Origene Technologies).
Rabbit Anti-EIF4A1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS627696

Immunohistochemistry (IHC) of anti-EIF4A1 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EIF4A1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-EIF4A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627696

Other Notes

Small volumes of anti-EIF4A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF4A1 purchase

MBS627696 is an antibody targeting EIF4A1. Also known as EIF4A1, NT (Eukaryotic Initiation Factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA Helicase eIF4A-1, DDX2A, EIF4A). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4A1. It may not necessarily be applicable to this product.

NCBI GI #
325197164
NCBI GeneID
1973
NCBI Accession #
NP_001191439.1
UniProt Primary Accession #
P60842
UniProt Secondary Accession #
P04765; Q5U018; Q61516; B2R6L8; D3DTP9
UniProt Related Accession #
P60842; Q59F68; Q9NZR9
GenBank Nucleotide #
NM_001204510.1
NCBI Official Full Name
eukaryotic initiation factor 4A-I isoform 2
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4A1
NCBI Official Symbol
EIF4A1
NCBI Official Synonym Symbols
DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
UniProt Protein Name
Eukaryotic initiation factor 4A-I
UniProt Synonym Protein Names
ATP-dependent RNA helicase eIF4A-1
Protein Family
Eukaryotic initiation factor
UniProt Gene Name
EIF4A1
UniProt Synonym Gene Names
DDX2A; EIF4A
UniProt Entry Name
IF4A1_HUMAN
NCBI Protein Information
eukaryotic initiation factor 4A-I; OTTHUMP00000135288; eukaryotic initiation factor 4AI; ATP-dependent RNA helicase eIF4A-1; eukaryotic translation initiation factor 4A, isoform 1

UniProt Comments for EIF4A1

EIF4A1: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with human cytomegalovirus/HHV-5 protein UL69. Helicase activity and function in translation are inhibited by interaction with PDCD4. Belongs to the DEAD box helicase family. eIF4A subfamily.

Protein type: Translation; Translation initiation; RNA-binding; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytoplasm; nucleus; cytosol

Molecular Function: mRNA binding; protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity; double-stranded RNA binding; helicase activity; RNA cap binding; ATP binding

Biological Process: poly(A) tail shortening; organ regeneration; cellular protein metabolic process; translation; viral reproduction; cytokine and chemokine mediated signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay
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Research Articles on EIF4A1

1. analysis of the tandem MA-3 region of Pdcd4 protein and characterization of its interactions with eIF4A and eIF4G

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF4A1 antibody

 Reference Product  PubMed Publications
 EIF4G1 antibody  >1 publications with EIF4A1 and EIF4G1

Pathways associated with anti-EIF4A1 antibody

 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Deadenylation Of MRNA Pathway antibodies  Deadenylation Of MRNA Pathway Diagram
 Deadenylation-dependent MRNA Decay Pathway antibodies  Deadenylation-dependent MRNA Decay Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 L13a-mediated Translational Silencing Of Ceruloplasmin Expression Pathway antibodies  L13a-mediated Translational Silencing Of Ceruloplasmin Expression Pathway Diagram
 Metabolism Of RNA Pathway antibodies  Metabolism Of RNA Pathway Diagram
 Metabolism Of MRNA Pathway antibodies  Metabolism Of MRNA Pathway Diagram

Diseases associated with anti-EIF4A1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with EIF4A1 and Neoplasms
 Adenocarcinoma Antibodies  >2 publications with EIF4A1 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >2 publications with EIF4A1 and Neoplasm Metastasis
 Hepatitis Antibodies  >2 publications with EIF4A1 and Hepatitis
 Lung Neoplasms Antibodies  >2 publications with EIF4A1 and Lung Neoplasms
 Lung Diseases Antibodies  >2 publications with EIF4A1 and Lung Diseases
 Inflammation Antibodies  >1 publications with EIF4A1 and Inflammation
 Skin Diseases Antibodies  >1 publications with EIF4A1 and Skin Diseases
 Liver Neoplasms Antibodies  >1 publications with EIF4A1 and Liver Neoplasms
 Liver Diseases Antibodies  >1 publications with EIF4A1 and Liver Diseases

Organs/Tissues associated with anti-EIF4A1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with EIF4A1 and Liver
 Skin Antibodies  >2 publications with EIF4A1 and Skin
 Lung Antibodies  >2 publications with EIF4A1 and Lung
 Ovary Antibodies  >1 publications with EIF4A1 and Ovary
 Pancreas Antibodies  >1 publications with EIF4A1 and Pancreas
 Embryonic Tissue Antibodies  >1 publications with EIF4A1 and Embryonic Tissue
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