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GPLD1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627733

GPLD1 is expressed in numerous tissues and cells and specifically cleaves GPI-anchored proteins. Liver has the highest level of GPI-PLD expression and is the primary organ contributing to GPLD1 in the serum. GPLD1 is abundant in serum in which it associates… More details

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Product Overview

Online Datasheet in PDF
Product Name
GPLD1, Polyclonal Antibody
Full Product Name
GPLD1, CT (Phosphatidylinositol-glycan-specific Phospholipase D, PI-G PLD, Glycoprotein Phospholipase D, Glycosyl-phosphatidylinositol-specific Phospholipase D, GPI-specific Phospholipase D, GPI-PLD, PIGPLD1)
Product Gene Name
anti-GPLD1 antibody
Product Synonym Names
Anti -GPLD1, CT (Phosphatidylinositol-glycan-specific Phospholipase D, PI-G PLD, Glycoprotein Phospholipase D, Glycosyl-phosphatidylinositol-specific Phospholipase D, GPI-specific Phospholipase D, GPI-PLD, PIGPLD1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human GPLD1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
92,336 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (24426060..24495433, complement). Location: 6p22.1
OMIM
602515
3D Structure
ModBase 3D Structure for P80108
Immunogen
Synthetic peptide selected from the C-terminal region of human GPLD1 (KLH).

Product Categories/Family for anti-GPLD1 antibody

Antibodies; Abs to Enzymes; Phospholipase
Applications Tested/Suitable for anti-GPLD1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-GPLD1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500

Western Blot (WB) of anti-GPLD1 antibody

GPLD2-R694 western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GPLD2 antibody detected the GPLD2 protein (arrow).
Rabbit Anti-GPLD1 Polyclonal Antibody – Cat# MBS627733

Immunohistochemistry (IHC) of anti-GPLD1 antibody

GPLD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPLD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-GPLD1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627733

Other Notes

Small volumes of anti-GPLD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPLD1 purchase

MBS627733 is an antibody targeting GPLD1. Also known as GPLD1, CT (Phosphatidylinositol-glycan-specific Phospholipase D, PI-G PLD, Glycoprotein Phospholipase D, Glycosyl-phosphatidylinositol-specific Phospholipase D, GPI-specific Phospholipase D, GPI-PLD, PIGPLD1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GPLD1. It may not necessarily be applicable to this product.

NCBI GI #
20269065
NCBI GeneID
2822
NCBI Accession #
CAC87068.1
UniProt Primary Accession #
P80108
UniProt Secondary Accession #
Q15127; Q15128; Q2M2F2; Q5T3Y0; Q7Z6T8; Q8TCV0; Q8WW82; Q96ID6; Q9H167; Q9H4M1; Q9UJC9
UniProt Related Accession #
P80108
NCBI Official Full Name
glycosylphosphatidylinositol phospholipase D
NCBI Official Synonym Full Names
glycosylphosphatidylinositol specific phospholipase D1
NCBI Official Symbol
GPLD1
NCBI Official Synonym Symbols
GPIPLD; PIGPLD; GPIPLDM; PIGPLD1
UniProt Protein Name
Phosphatidylinositol-glycan-specific phospholipase D
UniProt Synonym Protein Names
Glycoprotein phospholipase D; Glycosyl-phosphatidylinositol-specific phospholipase D
Protein Family
Phosphatidylinositol-glycan-specific phospholipase
UniProt Gene Name
GPLD1
UniProt Synonym Gene Names
PIGPLD1; PI-G PLD; GPI-PLD
UniProt Entry Name
PHLD_HUMAN
NCBI Protein Information
phosphatidylinositol-glycan-specific phospholipase D; GPI-PLD; PI-G PLD; GPI-specific phospholipase D; glycoprotein phospholipase D; glycosylphosphatidylinositol specific phospholipase D1, isoform 2

NCBI Summary for GPLD1

Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]
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UniProt Comments for GPLD1

GPLD1: This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. Belongs to the GPLD1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Secreted, signal peptide; Secreted; Phospholipase; EC 3.1.4.50

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: proteinaceous extracellular matrix; extracellular space; intracellular membrane-bound organelle; lysosomal membrane; cytoplasm; intracellular

Molecular Function: sodium channel regulator activity; glycosylphosphatidylinositol phospholipase D activity; phospholipase D activity

Biological Process: ossification; GPI anchor release; positive regulation of apoptosis; complement receptor mediated signaling pathway; positive regulation of membrane protein ectodomain proteolysis; phosphatidylcholine metabolic process; negative regulation of cell proliferation; cell migration during sprouting angiogenesis; cellular response to insulin stimulus; insulin receptor signaling pathway; response to glucose stimulus; positive regulation of secretion; chondrocyte differentiation; positive regulation of cytolysis
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Research Articles on GPLD1

1. genetic variation of GPLD1 appears to associate with circulating glycosylphosphatidylinositol-specific phospholipase D levels

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GPLD1 antibody

 Products by Pathway  Pathway Diagram
 Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway antibodies  Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway Diagram
 Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway antibodies  Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway Diagram

Diseases associated with anti-GPLD1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2 publications with GPLD1 and Inflammation
 Heart Diseases Antibodies  >1 publications with GPLD1 and Heart Diseases
 Liver Diseases Antibodies  >1 publications with GPLD1 and Liver Diseases

Organs/Tissues associated with anti-GPLD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with GPLD1 and Blood
 Liver Antibodies  >4 publications with GPLD1 and Liver
 Kidney Antibodies  >1 publications with GPLD1 and Kidney
 Pancreas Antibodies  >1 publications with GPLD1 and Pancreas
 Embryonic Tissue Antibodies  >1 publications with GPLD1 and Embryonic Tissue
 Lung Antibodies  >1 publications with GPLD1 and Lung
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